Development and validation of a RP- HPLC method for the quantitation studies of bromadiolone in Ratitox F

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of bromadiolone (hydroxycoumarins) in Ratitox F product – rodenticide. HPLC separation was carried out by reversed phase chromatography ODS 2 Hypersil C18 (250 mm x 4.6 mm i.e.; 5 ìm particle size), held in thermostat at 25°C. The mobile phase consisted of methanol/0.1% aqueous solution phosphoric acid (90/10v/v), with a flow rate of 1 ml/min and with UV detection at 265 nm. In order to validate the method, the following parameters have been investigated- linearity (r2 = 0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of Ratitox F – rodenticide

Determination of impurities and degradation products from veterinary medicinal products by HPLC method

The organic or inorganic impurities in the veterinary medicinal product can derive from starting materials, manufacturing process, incomplete purification, inappropriate storage. The acceptable levels of impurities in pharmaceuticals are estimated by comparison with standard solutions, according to the appropriate monographs. Forced degradation studies determine the stability of the method of dosage for the active compounds and for the entire finished product under excessive accelerated degradation conditions. They also provide information on degradation pathways and selectivity of analytical methods applied. The information provided by the degradation studies on the active compound and finished pharmaceutical product should demonstrate the specificity of the analytical method regarding impurities. Forced degradation studies should demonstrate that the impurities and degradation products generated do not interfere with the active compound. The current forced degradation methods consist of acid hydrolysis, basic hydrolysis, oxidation, exposure of the medicinal product to temperature and light. HPLC methods are an integral analytical instrument for the analysis of the medicinal product. The HPLC method should be able to separate, detect and quantify various specific degradation products that can appear after manufacture or storage of the medicinal product, as well as new elements appearing after synthesis. FDA and ICH guidelines recommend the enclosure of the results, including the chromatograms specific to the forced degradation-subjected medicinal product, in the documentation for marketing authorization. Using HPLC methods in forced degradation studies on medicinal products provides relevant information on the method of determination for the formulation of the medicinal product, synthesis product, packaging methods and storage

Multiple Bone Metastatic Invasion with Clinical Implications due to Ductal Mammary Carcinoma – Case report and Short Literature Review

Multidisciplinary approach of clinical cases in rehabilitations units can be very complex and divers. Method: We present a case of a woman 30 years old, whose oncological histo-ry begins 9 years ago, when the diagnosis of invasive ductal mammary infiltrating ca-nelular carcinoma grade I was made and treated by tumorectomy (lumpectomy). The pa-tient refused oncological treatment at that time and resorted to alternative medicine dur-ing a period of 7 years: diet without sugar and animal products; regular vitamin B17 treatments; almond kernel cure; high-dose intravenous vitamin C treatment for; intrave-nous ozone therapy; apitherapy with bee venom; oral administration of cannabis oil dur-ing the past four years until now; physical therapy. Results: The evolution is marked by the appearance of multiple bone metastatic lesions. The most worst clinical consequence was a hyperalgesic cervicobrachial neuralgia, due to multiple C6-T3 lytic lesions diag-nosed 2 years ago and C7 compression (close to spinal cord injury), situation which had as medical approach two surgeries interventions, radiotherapy and chemotherapy. Con-clusions: The rehabilitation program was adapted to every stage of clinical evolution and was marked by many complications. All the steps were made for functional improvement and for increase the quality of life

Enhancement of bone consolidation using high-frequency pulsed electromagnetic fields (HF-PEMFs): An experimental study on rats

In vitro studies showed that high-frequency pulsed electromagnetic fields (HF-PEMFs) increase the activity/expression of early and late osteogenic markers and enhance bone mineralization. The main aim of this study was to investigate the in vivo effects of HF-PEMFs on fracture healing using a rat model. A femur fracture was established by surgery in 20 male Wistar rats. Titanium nails were implanted to reduce and stabilize the fracture. After surgery, 20 rats were equally divided into untreated control and treated group (from the first postoperative day HF-PEMFs at 400 pulses/sec [pps] were applied for 10 minutes/day, for two weeks). Quantitative and qualitative assessment of bone formation was made at two and eight weeks following surgery and included morphological and histological analysis, serological analysis by ELISA, micro-computed tomography (micro-CT), and three-point bending test. At two weeks in HF-PEMF group, soft callus was at a more advanced fibrocartilaginous stage and the bone volume/total tissue volume (BV/TV) ratio in the callus area was significantly higher compared to control group (p = 0.047). Serum concentration of alkaline phosphatase (ALP) and osteocalcin (OC) was significantly higher in HF-PEMF group (ALP p = 0.026, OC p = 0.006) as well as the mechanical strength of femurs (p = 0.03). At eight weeks, femurs from HF-PEMF group had a completely formed woven bone with dense trabeculae, active bone marrow, and had a significantly higher BV/TV ratio compared to control (p = 0.01). HF-PEMFs applied from the first postoperative day, 10 minutes/day for two weeks, enhance bone consolidation in rats, especially in the early phase of fracture healing

Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of praziquantel and pyrantel pamoate in tablet dosage forms of TOTAL. HPLC separation wascarried out by reversed phase chromatography Betasil C18 (250 mm x 4,6mm i.d.; 5 5m particle size), held at 25°C respective Kromasil 60-5SIL. (250 mm x 4,6 mm i.d.; 5 5m particle size), held at 25°C. The mobile phase consisted of acetonitrile/distilled water (60/40v/v), run at flow rate of 1 mL/min and with UV detection at 210nm, respective acetonitrile/ 0,1% phosphoric acid aq. (60/40 v/v), run at flow rate of 1 mL/ min and with UV detection at 240 nm. Method validation investigated parameters such as linearity (r2=0,9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of TOTAL tablets

HPLC DETERMINATION OF D-PANTHENOL AND GLYCYRRHETINIC ACID FROM A HERBAL ANTI-HERPETIC SUPPLEMENT

HPLC was used for the quantitative determination of panthenol and glycyrrhetinic acid in an herbal antiherpetic supplement containing panthenol (B5 provitamin) and alcoholic extract of Glycyrrhiza glabra. For the determination of panthenol, the separation was carried out on a Kromasil 60-S Hilic D C18 column (250x4.6 mm, 5 µm), with methanol – 20 mM potassium phosphate dibasic, pH 6 (10:90, volume ratio) as the mobile phase. The detection wavelength was set at 210 nm. Flow was 1 mL·min-1 and the column temperature was maintained at (30±0.5) °C. For glycyrrhetinic acid, a Hypersil C18 BDS column (250x4.6 mm, 5 µm) with acetonitrile – 0.1% phosphoric acid in water (75:25, volume ratio) isocratic elution. The detection wavelength was set at 250 nm. Flow was 1 mL·min-1 and the column temperature was maintained at (30±0.5) °C

Development and validation of a RP–HPLC method for the quantization studies of metronidazole in tablets and powders dosage forms

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of metronidazole in tablets and powders. HPLC separation was carried out by reversed phasechromatography on Kromasil C18 (250 mm x 4.6 mm i.e.; 5 ìm particle size), held in thermostat at 25°C. The mobile phase consisted of methanol/ 0.1% phosphoric acid aq. (20/80v/v), with a flow rate of 1 ml/min and with UV detection at 317 nm. In order to validate the method, the following parameters have been investigated: linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of the active pharmaceuticalcompound in tablets and powders. This paper aimed to develop and validate an HPLC sensitive applicable method to determine the quantity of metronidazole in tablets and powders, contributing to the quality and safety control of these types of pharmaceutical preparations

Development and validation of a RP–HPLC method for quantization studies of albendazole suspension dosage forms of Rombendazol

An isocratic high-performance liquid chromatography (HPLC) procedure was developed and validated for the quantitative determination of albendazole in suspension of Rombendazole. HPLC separation was carried out by reversed phase chromatography on Kromasil C18 (150 mm x 4.6 mm i.e.; 5 μm particle size), held in thermostat at 25°C. The mobile phase consisted of Methanol/ Distilled water (65/35 v/v), with a flow rate of 1.2 ml/ min and UV detection at 308 nm. In order to validate the method, the following parameters have been investigated - linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection LOD and limit of quantification LOQ. The described method can be successfully applied for the analysis of the active pharmaceutical compound in suspensions

Development and validation of a RP-HPLC method for the quantisation studies of metronidazole and furazolidone from product Enteroguard M

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of metronidazole and furazolidone in tablet dosage forms of ENTEROGUARD M. HPLC separation was carried out by reversed phase chromatography Kromasil C18 (250mm x 4,6mm i.d.; 5μm particle size), held at 25°C. The mobile phase consisted of methanol/ 0,1% phosphoric acid aq. (20/80v/v), run at flow rate of 1 mL/ min and with UV detection at 317nm. Method validation investigated parameters such as linearity (r2 = 0.9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of ENTEROGUARD M tablets

Resource Allocation for PreuniversitaryInstitutions Based on Standard Costs

The educational system’s modernization implies the transfer of responsibilities regarding the multiplication of financing resources and expense coordination to the level of the institution’s management (the school director) and the direct participation of the local community and of the administrative authorities - mayors and town counselors- to the effort of ensuring the well-going of the training and educational process through school. It means, in the same time, creating a system designed to distribute equitably and rationally public money between educational units, and careful control of its expenditure. The educational system’s modernization also implies raising the control and intervention capacity of the decision factors on local and central levels as well as the correction of some errors or the removal of dysfunctionalities in budgetary planning and execution. Our study was founded on a survey type of scientific research, made on 50 preuniversitary educational institutions from Sibiu. The essential aims are ensuring the fund distribution based on standard costs and a real transfer towards the school of the attributions regarding the calculus of material and human resources and the accomplishment of a stimulant and efficient budgetary execution. Based on the facts presented by this paper, we notice the fact that the financing system based on standard costs and differential coefficients is not difficult to apply on the conditions of knowing it and the informational data collecting and processing to function normally.standard costs, public funds, preuniversitary educational institutions, differential coefficients, budget