Optimization Process for Bending a Periodic Structure: Start Ahead with Neural Networks

Optimizing high-dimensional designs requires essential efforts; bending of a radiating device is one of such examples. Such appearance appears in various applications like medical, space, radar, etc. Consequently, strong numerical methods are becoming necessary. In the present work, an optimized bent periodic structure, multiple-input and multiple-output (MIMO) antenna designed through a neural network (NN) is presented. The main goal of this optimization is to focus on the curvature of the antenna to find the suitable results for S-parameters both in terms of reflection S 11 , and transmission S 21 coefficients, and also total active reflection coefficient (TARC). Through the proposed method, the antenna configuration loaded by meandered slot and surrounded by printed meander line of different periodicities is designed and optimized in three frequency bands within the 12.94 GHz to 25 GHz range, namely, 12.94 - 14.79 GHz, 16.09 - 17.25 GHz, and 19.17 - 25 GHz

PRODUCTION AND PURIFICATION OF ANGIOTENSIN-CONVERTING ENZYME INHIBITOR BY SELECTED BACTERIAL STRAIN FOR CANCER THERAPY

Objective: The present study was planned to explore safer, innovative and economic Angiotensin-converting enzyme inhibitors (ACEi) from beef extract by the action of a proteolytic Micrococcus luteus. Cytotoxicity of the stable peptide was predicted using MCF-7 cell line in vitro.Methods: ACEi was purified by sequential steps of ethanol precipitation, ion exchange column chromatography (MonoQ) and gel filtration column chromatography (Sephadex G25). The apparent molecular mass was determined by SDS-PAGE. The anticancer property was analyzed by studying the cytotoxicity effects of angiotensin converting enzyme inhibitor using Breast cancer MCF-7 cell linesResults: The peptide was purified and molecular mass was determined as 4.5 kDa. The IC50 value of peptide was found to be 59.5 µg/ml. The DNA fragmentation was not observed in the treated cells. The purified peptide has demonstrated to induce apoptosis of cancer cell. The results proved that the peptide has the ability to be used for cancer therapy.Conclusion: The presence of ACE inhibition activities in the fermentation of beef extract using Micrococcus luteus has been investigated. The Peptide has been determined as an active compound that inhibited the activity of ACE. These properties indicate the possibilities of the use of purified protein as a potent anticancer agent.Keywords: Angiotensin-converting enzyme inhibitors, Micrococcus luteus, Anti-proliferative, Anti-metastatic, MCF-7 cell line, Anticancer activity

ISOLATION OF ANGIOTENSIN-CONVERTING ENZYME INHIBITOR PRODUCING BACTERIA FROM COW MILK

Objective: To evaluate the potential of protease producing organism for the production of Angiotensin I–converting enzyme (ACE) inhibitor by fermentation of various protein substrates.Methods: Bacterial strains were isolated from cow milk collected in Coimbatore, Tamil Nadu, India by using serial dilution technique, plated on nutrient agar medium. The identity of the strain was ascertained by 16s rRNA gene sequencing method and was submitted to the NCBI GenBank nucleotide database. Various substrates were screened for ACE inhibitor production by the fermentation with the isolated strain.Results: The isolated coded as BUCTL09, which showed a significant zone of clearance was selected and identified as Micrococcus luteus (KF303592.1). Among the seven substrates, only beef extract fermented broth showed an inhibition of 79% and was reported as the best substrate.Conclusion: In the search for non-toxic, and economic ACE inhibitors as an alternative to the synthetic drugs, many natural ACE inhibitors have been isolated from a microbial source. In the present study, isolate BUCTL09 was selected for the production of ACE inhibitor from the beef extract. Findings from this study lead us to investigate this potent ACE inhibitor further for its biological properties and to explore the impending efficacy of the ACE inhibitor which may conceivably be developed into a prospective drug

Binalarda güneş enerjisinden edilgen yararlanmada saydam yalıtım malzemeleri

Bu çalışmada, İzmir iklim koşulları altında binaların dış duvarlarının güneş ışınımı etkisi altında ısıl davranışları incelenmiştir. Deneyler, atmosferik koşullar altında yapılmıştır. Test örneği olarak; 4 mm cam, 4 cm saydam yalıtım, 3 cm dış sıva, 19 cm düşey boşluklu tuğla, 2 cm iç sıvadan oluşan duvar alınmıştır. Deney düzeneği; iç ortam özelliklerinin modellendiği ünite,örnek duvar kuruluşu,ölçüm ve kayıt sisteminden meydana gelmiştir. İç ortam özelliklerinin modellendirdiği ünitenin dış boyutları 1,1 m x 1,2 m x 1,2 m' dir. Ünitenin duvar ile temas eden yüzeyleri hariç tüm yüzeyleri yalıtılmıştır. Ünitenin ön yüzeyi, örnek duvar kuruluşu ile temas etmektedir Duvar kuruluşunun yüzey alanı 1,0 m x 1,0 m olup, yan yüzeyleri yalıtılmıştır. Duvar içinde, bir boyutlu ısı iletim koşulları gerçekleştirilmiştir. Bir yüzü iç ortam koşullarının modellendiği ünite ile temas eden duvarın diğer yüzeyi, dış ortam koşulları etkisi altında bırakılmıştır. Deneyler süresince gelen güneş ışınımı, iç ve dış ortam sıcaklıkları, duvar iç, dış yüzey sıcaklıkları ile katmanlar arasındaki sıcaklıklar sürekli olarak ölçülmüştür. Ölçüm sonuçları değerlendirilerek; saydam yalıtımlı duvar kuruluşundaki zaman gecikmesi,sönüm oranı, duvar kuruluş içinde farklı zamanlardaki sıcaklık dağılımları, duvarla depolanan enerji miktarları elde edilmiştir. Ayrıca model çalışası yapılarak duvarın ısıl davranışı teorik olarak incelenmiştir. Kurulan model sonuçları, deneysel çalışma verileri ile istatistiksel olarak karşılaştırılmıştır. Sonuç olarak; saydam yalıtımın opak yalıtım malzemeleri gibi ısı yalıtımı sağladığı, ayrıca opak yalıtımın aksine, yüksek güneş ışınım geçirgenlikleri nedeniyle güneş enerjisinden daha fazla katkı sağlanmasına olanak verdiği görülmüştür

Imminent angiotensin-converting enzyme inhibitor from microbial source for cancer therapy

Background: Drugs targeting Angiotensin I-converting enzyme (ACE) have been used broadly in cancer chemotherapy. The recent past coupled with our results demonstrates the effective use of ACE inhibitors (ACEi) as anticancer agents, and they are potentially relevant in deriving new inhibitors. Methods: Bacterial strains were isolated from cow milk collected in Coimbatore, Tamil Nadu, India and plated on nutrient agar medium. The identity of the strain was ascertained by 16s rRNA gene sequencing method and was submitted to the NCBI GenBank nucleotide database. Various substrates were screened for ACEi production by the fermentation with the isolated strain. ACEi was purified by sequential steps of ethanol precipitation, ion exchange column chromatography and gel filtration column chromatography. The apparent molecular mass was determined by SDS-PAGE. The anticancer property was analyzed by studying the cytotoxicity effects of ACEi using Breast cancer MCF-7 cell lines Results: The isolate coded as BUCTL09 was selected and identified as Micrococcus luteus. Among the seven substrates, only beef extract fermented broth showed an inhibition of 79% and was reported as the best substrate. The peptide was purified and molecular mass was determined. The IC50 value of peptide was found to be 59.5 μg/ ml. The purified peptide has demonstrated to induce apoptosis of cancer cell.Conclusions: The results of this study revealed that Peptide has been determined as an active compound that inhibited the activity of ACE. These properties indicate the possibilities of the use of purified protein as a potent anticancer agent

An extendable soft‐switched high step‐up converter with near zero‐ripple input current suitable for fuel cell‐powered applications

Abstract In this paper, an extendable high step‐up topology is presented for the low input voltage applications, that is, fuel cell (FC) source. The proposed converter eliminates the pulsating input current and reduces the high input current ripple. Thus, the lifetime of this non‐linear source and energy flow is significantly improved. The high output voltage is achieved by using voltage multiplier cells (VMC) and a coupled inductor (CI) with reduced elements. Moreover, an auxiliary circuit is applied due to restoring the energy of leakage inductance in the CI. Therefore, not only all power switching devices are turned on under zero voltage switching (ZVS) but also the input current ripple is remarkably reduced to zero. Furthermore, normalized semiconductors’ voltage stresses are diminished. The reverse recovery problem of diodes and their turn‐off power losses at high frequencies are alleviated owing to their zero current switching (ZCS) turn‐off. In this paper, the performance of the proposed structure is investigated and compared with the traditional converters considering their voltage gain and voltage stress. Eventually, a 500‐W prototype of the proposed converter is built and tested to validate mathematical analyses

Effect of high-dose vitamin D supplementation in combination with weight loss diet on glucose homeostasis, insulin resistance, and matrix metalloproteinases in obese subjects with vitamin D deficiency: a double-blind, placebo-controlled, randomized clinical trial

As there is limited and inconsistent evidence in potential role of vitamin D on insulin resistance and matrix metalloproteinases, this study aimed to examine the effect of vitamin D supplementation on glucose homeostasis, insulin resistance, and matrix metalloproteinases in obese subjects with vitamin D deficiency. A total of 44 participants with serum 25-hydroxyvitamin D (25(OH)D) level ≤ 50 nmol/L and body mass index (BMI) 30–40 kg/m2 were randomly allocated into receiving weight reduction diet with either 50 000 IU vitamin D3 pearl (n = 22) or placebo (n = 22) once weekly for 12 weeks. Primary outcomes were changes in fasting serum glucose (FSG), homeostasis model assessment of insulin resistance (HOMA-IR), quantitative insulin sensitivity check index (QUICKI), and matrix metalloproteinases (MMPs). Secondary outcomes were changes in weight, BMI, 25(OH)D, calcium, phosphorous and parathyroid hormone (PTH). Sun exposure and dietary intakes were also assessed. Serum levels of 25(OH)D3 increased significantly with a simultaneous decrease in serum concentration of PTH in the vitamin D group. Weight, BMI, FSG, and MMP-9 decreased significantly in both groups, and there were significant differences in changes in weight, serum 25(OH)D3, PTH, and MMP-9 levels between the groups. Within- and between-groups analysis revealed no significant differences in serum calcium, phosphorous, serum insulin, HOMA-IR, QUICKI, and MMP-2 after intervention. Our results indicated that improvement in vitamin D status resulted in greater reductions in weight and MMP-9 during weight loss. These preliminary results are sufficient to warrant a bigger study group.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author