アジュバント誘導性マウスリンパ管腫の有用性：リンパ管内皮に関してこの動物モデルから学べること

臨時増刊1号東京女子医科大学医学部解剖学・発生生物学講座 講座主任 江﨑太一教授退任記念特別

リンパ・リンパ管・リンパ組織の謎

日本語訳あり 臨時増刊1号東京女子医科大学医学部解剖学・発生生物学講座 講座主任 江﨑太一教授退任記念特別

“リンパ・リンパ管・リンパ組織の謎”への補足追記:脂肪に関連するリンパ管研究のための助言

日本語訳あり 臨時増刊1号東京女子医科大学医学部解剖学・発生生物学講座 講座主任 江﨑太一教授退任記念特別

Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics

BACKGROUND: The data on the embryonic origin of lymphatic endothelial cells (LECs) from either deep embryonic veins or mesenchymal (or circulating) lymphangioblasts presently available remain inconsistent. In various vertebrates, markers for LECs are first expressed in specific segments of embryonic veins arguing for a venous origin of lymph vessels. Very recently, studies on the mouse have strongly supported this view. However, in the chick, we have observed a dual origin of LECs from veins and from mesodermal lymphangioblasts. Additionally, in murine embryos we have detected mesenchymal cells that co-express LEC markers and the pan-leukocyte marker CD45. Here, we have characterized the mesoderm of murine embryos with LEC markers Prox1, Lyve-1 and LA102 in combination with macrophage markers CD11b and F4/80. RESULTS: We observed cells co-expressing both types of markers (e.g. Prox1 – Lyve-1 – F4/80 triple-positive) located in the mesoderm, immediately adjacent to, and within lymph vessels. Our proliferation studies with Ki-67 antibodies showed high proliferative capacities of both the Lyve-1-positive LECs of lymph sacs/lymphatic sprouts and the Lyve-1-positive mesenchymal cells. CONCLUSION: Our data argue for a dual origin of LECs in the mouse, although the primary source of embryonic LECs may reside in specific embryonic veins and mesenchymal lymphangioblasts integrated secondarily into lymph vessels. The impact of a dual source of LECs for ontogenetic, phylogenetic and pathological lymphangiogenesis is discussed

転載:新生仔マウス高濃度酸素暴露肺障害モデルにおける肺胞微小血管障害の形態学的特徴

臨時増刊1号東京女子医科大学医学部解剖学・発生生物学講座 講座主任 江﨑太一教授退任記念特別