DETECTION OF PATHOGENICITY GENES ENCODING ABILITY TO TOXIGENESIS IN ESCHERICHIA COLI ISOLATED FROM THE CHILDREN INTESTINAL BIOTOPE

Shiga toxins (stxs) are the virulence factor of enterohemorrhagic Escherichia coli (EHEC). Shiga toxin-producing Escherichia coli has been recognized as an important food-borne pathogen that causes severe diseases such as a hemolytic uremic syndrome (HUS). Provides information about the species composition of the major opportunistic organisms that inhabit this biotope. Using polymerase chain reaction (PCR) 96 strains of E. coli were examined for the presence of genes stx1 and stx2 coding the ability to toxigenesis. They were isolated from the children with functional disorders of the gastro-intestinal tract. Both pathogenicity genes present in the genome of E. coli with normal enzyme activity more often (stx1 in 24,2 % of genomes, stx2 in 9,1 %). The presence of stx1 and stx2 genes in different biochemical variants of E. coli allows to ascertain the fact of presence of a potentially pathogenicity reservoir in non-pathogenic forms of E. coli. Intestinal microflora is integral part of each individual. Further studying of its functions, the states interfering qualitative and quantitative composition of microorganisms, colonizing human gastrointestinal tract, as well as the pathogenic potential

REGIONAL FEATURES OF BIFIDOBACTERIA ANTAGONISTIC ACTIVITY IN INTESTINAL MICROBIOTA OF CHILDREN LIVING IN SIBERIA

Antagonistic properties of bifidobacteria to opportunistic microorganisms in vitro and mechanism of antagonistic activity decreasing were studied and. analyzed. Great percentage of bifidobacteria regional population strains with low antagonistic activity to transitional opportunistic microorganisms is registered. It results to colonization. resistance decreasing and. requires development of the preventive probiotics therapy methods to correct

Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age

Pelvic inflammatory diseases occupy a special place in the structure of general morbidity, and are polymicrobial in nature with dominance of opportunistic microorganisms, in particular bacteria of the family Enterobacteriaceae. The aim was to study the composition of the vaginal microbiota in women of reproductive age with pelvic inflammatory diseases, as well as to determine the sensitivity of isolated microorganisms to antibiotics and bacteriophages. The study included 70 women of reproductive age, among them 37 were diagnosed with colpitis and cervicitis, 33 women in the comparison group (women screened for a diagnosis). Isolated microorganisms were identified by abdominoperineal methods, including the disk diffusion method to determine the sensitivity of microorganism cultures of Enterobacteriaceae family to antibiotics, and the method of crosses (evaluation of lytic activity of bacteriophages by the number of crosses) to determine the sensitivity to specific therapeutic bacteriophages. Vaginal biocenosis was characterized by deficit of lactobacilli (< 106 CFU/ml in 100 %), the presence of conditionally pathogenic microflora: bacteria of Enterobacteriaceae family, coccal flora and Candida fungi. From 60.0 to 89.3 % of Enterobacteria strains were resistant to aminoglycosides and quinolones, but also had a low level of sensitivity to therapeutic bacteriophages. The obtained data indicate the reduction of colonization resistance of vaginal mucosa in pelvic inflammatory diseases and specify the need to use medicinal drugs only under medical supervision to prevent clinically significant drug resistance

MICROECOLOGICAL LANDSCAPE OF DIFFERENT BIOTOPES IN CHILDREN LIVING IN IRKUTSK REGION

Intestinal, nasopharyngeal microbiocenoses peculiarities and bifidobacteria species composition in children living in the Irkutsk region industrial cities under existing technogenic loading conditions have been studied. Major microbiocenosis variants in children living in the cities with the different levels of the atmospheric air pollution have been determine

Detection of some genes encoding pathogenicity factors in the typical isolates of Escherichia coli

Using polymerase chain reaction 316 strains of E. coli (strains with normal enzymatic activity, strains with weak enzymatic activity and strains with hemolytic activity) were examined for the presence of pathogenicity genes. They were isolatedfrom healthy children and children with functional disorders of the gastro-intestinal tract. The studies have shown that strains of Escherichia coli have pathogenic potential, as evidenced by the presence of genetic pathogenicity markers in them

SPECIES DIVERSITY OF BIFIDOBACTERIA IN CHILDREN WITH FUNCTIONAL DISORDERS OF GASTROINTESTINAL TRACT

The main aim of this research was to identify species ratio and compatibility in children with functional disorders of gastrointestinal tract (GIT) using PCR method, with, species-specific primers for bifidobacteria. The assessment of intestinal microbiota symbionts composition, was made simultaneously. Dysbiosis changes of I—II degree in large intestine microbiocenosis in 86 children examined, were detected. The definition, of bifidobacteria species profile with, the dominance of B. longum, B. catenulatum. and B. bifidum. will allow to optimize the treatment of this pathology

ANTITOXIC ANTIDIFTHERIAL ANTIBODIES IN BLOOD SERA OF HEALTHY ADULTS UNDER DIFFERENT VACCINATION SCHEMES

In this work intensity of immunity to diphtheria on the basis of indicating antitoxic antidiphtherial antiboies level in the blood sera of healthy adults aged 20 to 51 and elder, living in England, Italy, Latvia, Lithuania, Russia and Finland is analyzed. Diphtheria disease incidence shift through the epidemic in 1993—1996 in Russia to the elder age groups had resulted in inclusion of obligatory adults revaccination each 10 years after the last revaccination of teenagers in 14 year in 1998. Chosen tactics of vaccinal prevention of diphtheria didn't find reflection in National prophylactic immunization calendars of other countries including European, except Finland. On the basis of the received analysis for optimization of diphtheria vaccinal prevention, authors offer: to transfer a revaccination of children from age of 18 months to age of 3—6 years (according to the scheme in Italy, England, Finland); to carry out vaccination of adults individually depending on antitoxic antibodies concentration in blood sera

ANTITOXIC ANTIDIPHTHERIAL ANTIBODIES IN BLOOD SERUM OF HEALTHY ADULTS UNDER DIFFERENT VACCINATION SCHEMES

Intensity of immunity to the diphtheria is analyzed, in the article according to the levels of antitoxic antibodies in blood serum, of healthy adults from 20 to 51 years and older who live in England, Italy, Latvia, Lithuania, Russia and. Finland. In Russia displacement of morbidity during the epidemic of 1993—1996 to the older age groups caused, inclusion of compulsory revaccination of adults every 10 years after the latest vaccination, of teenagers in 14 years in National calendar of o prophylactic vaccination, in 1998. Chosen tactics of vaccinal prevention, wasn't included, in National calendars of o prophylactic vaccination, of other countries including European except for Finland. On the basis of the analysis for the optimization, of vaccinal prevention, of diphtheria the authors propose: to transfer revaccination of children from the age of 18 months to the age of 3—6 years (like in Italy, England, and. Finland), to realize vaccination, of adults individually taking into account the content of antitoxic antibodies in blood serum.. Key words: diphtheria, vaccinal prevention, antitoxic antibodie

Microecological and associative structure of intestinal biocenosis in children with functional gastrointestinal disorders

The article is devoted to one of actual problems of gastroenterology - study of microbiocenosis of gastrointestinal tract in functional disorders. Functional gastrointestinal disorders (FGID) or minimal dysfunctions of digestion are frequent in the first months of child's life and provoke anxiety both in parents and in pediatricians. Aim: to explore the microbial landscape and the associative structure of the microbiota of the large intestine in children with functional disorders of the gastrointestinal tract. Subjects and methods: Intestinal microbiota composition of 225 children with functional gastrointestinal disorders and 100 healthy children was studied using bacteriological method. Results. The 88.4 % frequency of detection of intestinal eubiosis disorders is discussed. Information is provided on the species composition of the major opportunistic organisms that inhabit this biotope. According to the results, decrease of bifidobacteria amount in structure of large intestine microbiota increases rate of detection of opportunistic microorganisms - predominantly, Klebsiella genus bacteria and Staphylococcus aureus. The intestinal microbial community is dominated by multicomponent transient association. The frequency of detection of pathogenic Staphylococcus in children under one year is discussed in detail. Conclusions. A distinctive feature of the intestinal biocenosis microecological status is significantly greater spread of enterococci than in healthy children, which can be a risk for the emergence of strains with the presence of a number of pathogenecity factors that cause infectious processes

CONTENTS DIPHTHERIA ANTITOXIC AND ANTIBACTERIAL ANTIBODIES IN THE SERUM OF HEALTHY ADULTS

In the article using the binomial distribution analysis of the intensity of рориlation antitoxic (AT) and antibacterial (AB) of immunity to diphtheria in terms of specific diphtheria antibodies in the sera of healthy adults aged 20 to 51 years and. older, living in Russia and. Italy. The latter, in contrast to Russia, during the epidemic was only a few cases of diphtheria. For the population of people from Italy the optimal parameters of tension antibody immunity (bimodal distribution of the concentrations of AT antibodies) are determined, whereas for the adult population of Russia experienced an abnormally high, overvoltages antibody immunity (lack of bimodal distribution). The level of concentration of antibody isotypes AB M, G, A in both countries match the total synthesis of immunoglobulin isotypes in the ontogeny of these: thus, in the sera of adults aged 20 to 40 years AB antibody IgG prevail. Low concentrations of antitoxin in the blood of most adults living in Italy, recharged high AB IgG in these same serum, and. optimal concentrations of AB IgM and. IgA. This indicates the possibility lifting the mass revaccination of adults, and. creating an individual approach to their boosters. Optimal bimodal distribution of AB antibodies in the population of healthy adults, both in Russia and. in Italy, is an additional protection to the diphtheria antitoxic resistance