Attacker-Induced Traffic Flow Instability in a Stream of Automated Vehicles

Highway systems world wide continue to see an ever increased number of vehicles and subsequently a rise in congested traffic. This results in longer commute times, wasted energy as vehicles idle in stop and go traffic, and increases the risk of accidents. In short, increased congestion costs time and money. These issues have prompted much research into Automated Highway Systems (AHS). In AHS vehicles using computer algorithms can safely travel at much smaller inter-vehicle distances than human drivers are capable of. This increases the capacity of existing highway systems. Sensors aboard each vehicle make this possible by monitoring their surroundings. Vehicles equipped with Adaptive Cruise Control (ACC) are capable of this type of close proximity travel. ACC packages are becoming common as a standard package on many mid-priced vehicles. Another form of automation, Cooperative Adaptive Cruise Control (CACC), which utilizes wireless communication between vehicles, has been proposed and will likely become available within the next couple decades. CACC allows each vehicle to communicate their intended speed or position changes to surrounding vehicles, further decreasing the possibility of collisions. These automation methods are proposed to reduce driver stress, increase highway throughput, and decrease accident rates. However, the fact that vehicles are being automated creates new opportunities for malicious individuals to wreak havoc on society. This research investigates the possibility that some vehicles on the highway might be under the control of malicious individuals who have modified their automated control systems to negatively affect vehicles around them. These malicious actors might also exploit the wireless communication of CACC vehicles and hack their control algorithms, causing them to become unstable. These hacked vehicles could become passive participants in the attack unbeknownst to the driver of the vehicle. The result of such attacks could be congested traffic, rapid changes in acceleration causing drivers discomfort, or multi-vehicle collisions. Such attacks could effectively negate the benefits of implementing AHS. The goal of this work is to bring to light possible weaknesses in the proposed systems so they can be rectified before becoming an issue to the public at large

Tethering polypeptides through bifunctional PEG cross-linking agents to probe protein function: application to ATP synthase.

Chemical crosslinking mediated by short bifunctional reagents has been widely used for determining physical relationships among polypeptides in multisubunit proteins, but less often for functional studies. Here we introduce the approach of tethering polypeptides by using bifunctional reagents containing a lengthy, flexible PEG linker as a form of crosslinking especially suited to functional analyses. The rotary molecular motor ATP synthase was used as a model subject. Single cysteine residues were introduced into selected positions of ATP synthase epsilon subunit, a component of the rotor subcomplex of the enzyme, and the unrelated maltose binding protein (MBP), then the two purified recombinant proteins were crosslinked by means of a dimaleimido-PEG cross-linking agent. Following purification, the epsilon-PEG-MBP was incorporated into membrane-bound ATP synthase by reconstitution with epsilon-depleted F(1)-ATPase and membrane vesicles that had been stripped of endogenous F(1). ATP synthase reconstituted using epsilon-PEG-MBP had reduced ATP hydrolytic activity that was uncoupled from the pumping of H(+), indicating the physical blockage of rotation of the gammaepsilonc(10) rotor by the conjugated MBP, whereas enzyme reconstituted with epsilon-PEG was normal. These results directly demonstrate the feasibility of studying mechanistic features of molecular motors through PEG-based conjugation of unrelated proteins. Since tethering polypeptides provides a means of maintaining proximity without directly specifying or modifying interactions, application of the general method to other types of protein functional studies is envisioned

How Fast Do Personal Computers Depreciate? Concepts and New Estimates

This paper provides new estimates of depreciation rates for personal computers using an extensive database of prices of used PCs. Our results show that PCs lose roughly half their remaining value, on average, with each additional year of use. We decompose that decline into age-related depreciation and a revaluation effect, where the latter effect is driven by the steep ongoing drop in the constant-quality prices of newly-introduced PCs. Our results are directly applicable for measuring the depreciation of PCs in the National Income and Product Accounts (NIPAs) and were incorporated into the December 2003 comprehensive NIPA revision. Regarding tax policy, our estimates suggest that the current tax depreciation schedule for PCs closely tracks the actual loss of value in a zero-inflation environment. However, because the tax code is not indexed for inflation, the tax allowances would be too small in present value for inflation rates above the very low level now prevailing.

36 degree step size of proton-driven c-ring rotation in FoF1-ATP synthase

Synthesis of the biological "energy currency molecule" adenosine triphosphate ATP is accomplished by FoF1-ATP synthase. In the plasma membrane of Escherichia coli, proton-driven rotation of a ring of 10 c subunits in the Fo motor powers catalysis in the F1 motor. While F1 uses 120 degree stepping, Fo models predict a step-by-step rotation of c subunits 36 degree at a time, which is here demonstrated by single-molecule fluorescence resonance energy transfer.Comment: 8 pages, 1 figur

Exploring views on satisfaction with life in young children with chronic illness: an innovative approach to the collection of self-report data from children under 11

The objective of this study was to explore young children’s views on the impact of chronic illness on their life in order to inform future development of a patient-based self-report health outcome measure. We describe an approach to facilitating self-report views from young children with chronic illness. A board game was designed in order to obtain qualitative data from 39 children with a range of chronic illness conditions and 38 healthy controls ranging in age from 3 to 11 years. The format was effective in engaging young children in a self-report process of determining satisfaction with life and identified nine domains. The board game enabled children aged 5–11 years with chronic illness to describe the effects of living with illness on home, family, friends, school and life in general. It generated direct, non-interpreted material from children who, because of their age, may have been considered unable or limited their ability to discuss and describe how they feel. Obtaining this information for children aged 4 and under continues to be a challenge

Scholarship Programs for Vertical Transfers in Engineering and Engineering Technology

This paper introduces two scholarship projects funded by the National Science Foundation that focus on students who transfer at the 3rd year level from 2-year schools to the engineering and engineering technology BS programs at our university. The objectives of both the projects are: (i) to expand and diversify the engineering/technology workforce of the future, (ii) to develop linkages and articulations with 2-year schools and their S-STEM programs, (iii) to provide increased career opportunities and job placement rates through mandatory paid co-op experiences, and (iv) to serve as a model for other universities to provide vertical transfer students access to the baccalaureate degree. The Transfer Pipeline (TiPi) project awarded 25 new scholarships per year from 2012 to 2014 to a total of 75 engineering and engineering technology transfer students. By the end of Fall 2017, 66 (88%) scholars have graduated, 5 (7%) are in process of completing their degrees, and only 4 (5%) left our university, for a 95% retention rate. The paper describes our successes and challenges. The Vertical Transfer Access to the Baccalaureate (VTAB) project recruited its first group of 25 students in Fall 2017 with the goal of recruiting a total of 78 vertical transfers over the next three years. An additional goal of the VTAB project is to conduct research and generate knowledge about the VTAB project elements that will be essential for the success of vertical transfer programs at other universities. The paper describes the research instruments, and the results from an online survey and a focus group interview of the first cohort of VTAB scholars

Promoter keyholes enable specific and persistent multi-gene expression programs in primary T cells without genome modification

Non-invasive epigenome editing is a promising strategy for engineering gene expression programs, yet potency, specificity, and persistence remain challenging. Here we show that effective epigenome editing is gated at single-base precision via 'keyhole' sites in endogenous regulatory DNA. Synthetic repressors targeting promoter keyholes can ablate gene expression in up to 99% of primary cells with single-gene specificity and can seamlessly repress multiple genes in combination. Transient exposure of primary T cells to keyhole repressors confers mitotically heritable silencing that persists to the limit of primary cultures in vitro and for at least 4 weeks in vivo, enabling manufacturing of cell products with enhanced therapeutic efficacy. DNA recognition and effector domains can be encoded as separate proteins that reassemble at keyhole sites and function with the same efficiency as single chain effectors, enabling gated control and rapid screening for novel functional domains that modulate endogenous gene expression patterns. Our results provide a powerful and exponentially flexible system for programming gene expression and therapeutic cell products