Preparation as Prevention – Reducing Morbidity and Mortality in the Vermont Outdoors

While precipitants and outcomes of wilderness medical emergencies are numerous and diverse, wilderness rescues in Vermont are most often called for individuals or groups inadequately prepared for conditions or contingencies. In many cases, failure to bring one of a few key items such as equipment for navigation or illumination after dark can create a preventable emergency with subsequent requirement for rescue or retrieval.https://scholarworks.uvm.edu/fmclerk/1522/thumbnail.jp

Nutrition Knowledge Assessment of Lund Family Center Residents

Introduction. Staff at the Lund Family Center report that there may be knowledge deficits, with regards to nutrition, among the Center’s residents—primarily pregnant/ parenting women with substance abuse and mental health issues. Before considering intervention options, we wanted to identify the specifics of the residents’ knowledge gaps. Methods. We administered paper versions of a revised General Nutrition Knowledge Questionnaire (GNKQ-R) Section 2 to 21 Lund residents to explore nutri- tion knowledge as compared to groups more representative of the general population. The GNKQ-R Section 2 is an externally validated, efficacious tool that specifically as- sesses knowledge of food groups and nutrients. Demographic data and free-response personal assessments of health were also collected. Results. The study population scored significantly lower than a UK population (p=0.002) previously analyzed using the questionnaire, however, the Lund residents scored significantly higher than a CA population (p=0.0001). There were statistically insignificant positive relationships between age, education level, and self-reported health status, in addition to slightly lower performances among those with “Single” relationship status. Conclusions. Though demonstrated by a small, homogenous population, the statistically significant nutrition knowledge deficit of the Lund Family Center residents, relative to the referenced UK study, warranted intervention. A brief nutrition curriculum composed of 16 focused modules was developed for future administration. The modules were oriented towards family nutrition, with content including such topics as breastfeeding advice, including children in meal-making, and macronutrient basics. These modules will be delivered to Lund residents in 2018.https://scholarworks.uvm.edu/comphp_gallery/1261/thumbnail.jp

Symbiotic Legume Nodules Employ Both Rhizobial Exo- and Endo-Hydrogenases to Recycle Hydrogen Produced by Nitrogen Fixation

BACKGROUND: In symbiotic legume nodules, endosymbiotic rhizobia (bacteroids) fix atmospheric N(2), an ATP-dependent catalytic process yielding stoichiometric ammonium and hydrogen gas (H(2)). While in most legume nodules this H(2) is quantitatively evolved, which loss drains metabolic energy, certain bacteroid strains employ uptake hydrogenase activity and thus evolve little or no H(2). Rather, endogenous H(2) is efficiently respired at the expense of O(2), driving oxidative phosphorylation, recouping ATP used for H(2) production, and increasing the efficiency of symbiotic nodule N(2) fixation. In many ensuing investigations since its discovery as a physiological process, bacteroid uptake hydrogenase activity has been presumed a single entity. METHODOLOGY/PRINCIPAL FINDINGS: Azorhizobium caulinodans, the nodule endosymbiont of Sesbania rostrata stems and roots, possesses both orthodox respiratory (exo-)hydrogenase and novel (endo-)hydrogenase activities. These two respiratory hydrogenases are structurally quite distinct and encoded by disparate, unlinked gene-sets. As shown here, in S. rostrata symbiotic nodules, haploid A. caulinodans bacteroids carrying single knockout alleles in either exo- or-endo-hydrogenase structural genes, like the wild-type parent, evolve no detectable H(2) and thus are fully competent for endogenous H(2) recycling. Whereas, nodules formed with A. caulinodans exo-, endo-hydrogenase double-mutants evolve endogenous H(2) quantitatively and thus suffer complete loss of H(2) recycling capability. More generally, from bioinformatic analyses, diazotrophic microaerophiles, including rhizobia, which respire H(2) may carry both exo- and endo-hydrogenase gene-sets. CONCLUSIONS/SIGNIFICANCE: In symbiotic S. rostrata nodules, A. caulinodans bacteroids can use either respiratory hydrogenase to recycle endogenous H(2) produced by N(2) fixation. Thus, H(2) recycling by symbiotic legume nodules may involve multiple respiratory hydrogenases