Epidémiologie des bactériémies à bacille Gram négatif (étude sur 5 ans en réanimation au Centre hospitalier de Versailles)

PARIS-BIUP (751062107) / SudocSudocFranceF

Detection of pecluviruses

Indian peanut clump virus (IPCV) and Peanut clump virus (PCV) cause significant losses in groundnut crops . IPCV and PCV infect also a wide range of dicotyledonous and monocotyledonous hosts. Virus infection often has few or no symptoms in other hosts, such as cereals and serological detection is hampered by the significant differences among the amino acid composition of pecluvirus coat proteins. The availability of non-serological sensitive and broad-spectrum detection methods is therefore essential for the epidemiological studies and for the management of peanut clump disease. Reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the detection of both IPCV and PCV. The chapter describes pecluviruses and the molecular diagnostic techniques proposed for their identification and characterization, from serological to polymerase-based methods. It shows that pecluviruses can be present in graminaceous hosts, even when no symptoms are apparent, and highlights the risks of spreading disease by seed and soil movements

Characterization, diagnosis & management of plant virusesVolume 3. Vegetable and pulse crops : Chapter 5 - Pecluviruses

Indian peanut clump virus (IPCV) and Peanut clump virus (PCV) cause significant losses in groundnut crops . IPCV and PCV infect also a wide range of dicotyledonous and monocotyledonous hosts. Virus infection often has few or no sumptoms in other hosts, such as cereals and serological detection is hampered by the significant differences among the amino acid composition of pecluvirus coat proteins. The availability of non-serological, sensitive and broad-spectrum detection methods is therefore essential for the epidemiological studies and for the management of peanut clump disease. Reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the detetion of both IPCV and PCV. The chapter describes pecluviruses and the molecular diagnostic tehniques proposed for their identification and charaterization, from serological to polymerase-based methods. It shows that pecluviruses can be present in graminaceous hosts, even when no symptoms are apparent, and highlights the risks of spreading disease by seed and soil movements

Acquisition and transmission of peanut clump virus by polymyxa graminis on cereal species

The objective of this study was to investigate the specificity of the interactions between Polymyxa graminis, Peanut clump virus (PCV), and cereals, particularly the acquisition and the transmission of the virus by three P. graminis formae speciales. A new strategy has been developed: it involves using sugarcane as the common host for both the virus and its vector in order to produce the viruliferous zoospores of P. graminis f. sp. subtropicalis, temperata, and tropicalis that were then inoculated on cereal species. This experiment enabled the role of P. graminis f. sp. tropicalis and subtropicalis zoospores in PCV transmission to be demonstrated. The efficiency of this transmission was shown to vary, depending on the P. graminis special forms. Interestingly, the high transmission of the PCV isolate from Burkina Faso by an isolate of P. graminis f. sp. tropicalis from Niger on pearl millet suggests that there is a coevolution mechanism in this pathosystem. The study also provides evidence that the host plant species in which Polymyxa zoospores are produced could affect the infectivity of the vector. Finally, using Polymyxa quantitation by quantitative reverse-transcription polymerase chain reaction and in situ observations of the virus, the study demonstrates the independence of the development of PCV and its vector in the host plants

Whole-Body and Microenvironmental Localization of Radium-223 in Naïve and Mouse Models of Prostate Cancer Metastasis.

Bone-metastatic, castration-resistant prostate cancer (bmCRPC) represents a lethal stage of the most common noncutaneous cancer in men. The recent introduction of Radium-223 dichloride, a bone-seeking alpha particle (α)-emitting radiopharmaceutical, demonstrates statistically significant survival benefit and palliative effect for bmCRPC patients. Clinical results have established safety and efficacy, yet questions remain regarding pharmacodynamics and dosing for optimized patient benefit