Use of Mechanical Equipment in Mine Clearance

In recent years, mechanical equipment has become more and more prominent in demining programs around the world. This article provides an overview of mechanical demining equipment and highlights the involvement of the Geneva International Centre for Humanitarian Demining (GICHD) in promoting such equipment

Throwing Out Mines: The Effects of a Flail

The authors discuss a study conducted on flail machines to prove the effectiveness of this technology in destroying anti-personnel mines

Dissociation of β2m from MHC class I triggers formation of noncovalent transient heavy chain dimers

At the plasma membrane of mammalian cells, major histocompatibility complex class I molecules (MHC-I) present antigenic peptides to cytotoxic T cells. Following the loss of the peptide and the light chain beta-2 microglobulin ($β_2m$, encoded by B2M), the resulting free heavy chains (FHCs) can associate into homotypic complexes in the plasma membrane. Here, we investigate the stoichiometry and dynamics of MHC-I FHCs assemblies by combining a micropattern assay with fluorescence recovery after photobleaching (FRAP) and with single-molecule co-tracking. We identify non-covalent MHC-I FHC dimers, with dimerization mediated by the $α_3$ domain, as the prevalent species at the plasma membrane, leading a moderate decrease in the diffusion coefficient. MHC-I FHC dimers show increased tendency to cluster into higher order oligomers as concluded from an increased immobile fraction with higher single-molecule colocalization. In vitro studies with isolated proteins in conjunction with molecular docking and dynamics simulations suggest that in the complexes, the $α_3$ domain of one FHC binds to another FHC in a manner similar to that seen for $β_2m$