Dual laser excitation of a photochromic system: application to DODCI

A technique based on a combination of pump-and-probe fluorescence and absorption detection was developed in order to obtain the emission parameters of the pholoisomeric (P) species of dyes with noncompletely overlapped absorption spectra of P and the starting species (N ). Near total population transfer to the ground state of P was achieved using a continuous wave high fluence pump laser. A pulsed laser probed this population in its absorption region in such a way that the pulsed emission corresponded to fluorescence from P. Absorption measurements allowed us to follow the P population and to establish its relationship with pump fluence and wavelength. The direct monitoring of the P concentration increased the accuracy of the previously reported values for several photophysical parameters. Temperature dependent fluorescence quantum yield and lifetime, emission spectrum, and Arrhenius parameters of the nonradiative decay processes of P from 3,3' diethyloxa dicarbocyanine iodide (DODCI) are given. The mechanism for the direct transformation between N and P through the singlet excited states of DODCI is reevaluated.Centro de Investigaciones Óptica

Dual laser excitation of a photochromic system: application to DODCI

A technique based on a combination of pump-and-probe fluorescence and absorption detection was developed in order to obtain the emission parameters of the pholoisomeric (P) species of dyes with noncompletely overlapped absorption spectra of P and the starting species (N ). Near total population transfer to the ground state of P was achieved using a continuous wave high fluence pump laser. A pulsed laser probed this population in its absorption region in such a way that the pulsed emission corresponded to fluorescence from P. Absorption measurements allowed us to follow the P population and to establish its relationship with pump fluence and wavelength. The direct monitoring of the P concentration increased the accuracy of the previously reported values for several photophysical parameters. Temperature dependent fluorescence quantum yield and lifetime, emission spectrum, and Arrhenius parameters of the nonradiative decay processes of P from 3,3' diethyloxa dicarbocyanine iodide (DODCI) are given. The mechanism for the direct transformation between N and P through the singlet excited states of DODCI is reevaluated.Centro de Investigaciones Óptica

Dual laser excitation of a photochromic system: application to DODCI

A technique based on a combination of pump-and-probe fluorescence and absorption detection was developed in order to obtain the emission parameters of the pholoisomeric (P) species of dyes with noncompletely overlapped absorption spectra of P and the starting species (N ). Near total population transfer to the ground state of P was achieved using a continuous wave high fluence pump laser. A pulsed laser probed this population in its absorption region in such a way that the pulsed emission corresponded to fluorescence from P. Absorption measurements allowed us to follow the P population and to establish its relationship with pump fluence and wavelength. The direct monitoring of the P concentration increased the accuracy of the previously reported values for several photophysical parameters. Temperature dependent fluorescence quantum yield and lifetime, emission spectrum, and Arrhenius parameters of the nonradiative decay processes of P from 3,3' diethyloxa dicarbocyanine iodide (DODCI) are given. The mechanism for the direct transformation between N and P through the singlet excited states of DODCI is reevaluated.Centro de Investigaciones Óptica

"In vitro" comparative immune effects of different titanium compounds.

Exposure to Ti compounds is today an occupational and environmental health hazard. Object of this study was to determine "in vitro" effects of different Ti salts on cultured human peripheral blood mononuclear cells (PBMC) proliferation and cytokine release. 10−4 and 10−7 M Ti compounds did not modify spontaneous PBMC proliferation. Ti dioxide (a biocompatible material and sunscreen component) did not exert effects on phytoemagglutinin (PHA) stimulated PBMC proliferation and on PHA stimulated IFN-γ and TNF-α release from PBMC. On the other hand, 10−4 M Ti oxalate (with wide industrial applications) and Ti ascorbate (used mainly in agriculture) inhibited about 70 % the PHA stimulated PBMC proliferation; both these Ti compounds at 10−4 and 10−7 M concentrations significantly inhibited TNF-α release, while only Ti oxalate inhibited that of IFN-γ. Titanocene (used in chemotherapy) did not exert effects on PBMC proliferation but markedly inhibited IFN-γ and TNF-α release. On the whole, this study demonstrates that Ti dioxide is not immunotoxic; Ti oxalate shows marked immunotoxicity; titanocene exerts selective toxicity on cytokine release but not on PBMC proliferation, while Ti ascorbate affects TNF-α release from PBMC but not IFN-γ release. In conclusion, these data show that immunotoxicity of Ti depends on speciation

Biocompatible cellulose nanocrystal-based Trojan horse enables targeted delivery of nano-Au radiosensitizers to triple negative breast cancer cells

: A hybrid cellulose-based programmable nanoplatform for applications in precision radiation oncology is described. Here, sugar heads work as tumor targeting moieties and steer the precise delivery of radiosensitizers, i.e. gold nanoparticles (AuNPs) into triple negative breast cancer (TNBC) cells. This "Trojan horse" approach promotes a specific and massive accumulation of radiosensitizers in TNBC cells, thus avoiding the fast turnover of small-sized AuNPs and the need for high doses of AuNPs for treatment. Application of X-rays resulted in a significant increase of the therapeutic effect while delivering the same dose, showing the possibility to use roughly half dose of X-rays to obtain the same radiotoxicity effect. These data suggest that this hybrid nanoplatform acts as a promising tool for applications in enhancing cancer radiotherapy effects with lower doses of X-rays

Mediterranean diet impact on cardiovascular diseases: a narrative review

: Cardiovascular disease (CVD) accounts for more than 17 million deaths per year worldwide. It has been estimated that the influence of lifestyle on CVD mortality amounts to 13.7% for smoking, 13.2% for poor diet, and 12% for inactive lifestyle. These results deeply impact both the healthy status of individuals and their skills in working. The impact of CVD on productivity loss accounts for the 24% in total costs for CVD management.Mediterranean diet (MedD) can positively impact on natural history of CVD. It is characterized by a relatively high consumption of inexpensive and genuine food such as cereals, vegetables, legumes, nuts, fish, fresh fruits, and olive oil as the principal source of fat, low meat consumption and low-to-moderate consumption of milk, dairy products, and wine.Its effects on cardiovascular health are related to the significant improvements in arterial stiffness. Peripheral artery disease, coronary artery disease, and chronic heart failure are all positively influenced by the MedD. Furthermore, MedD lowers the risk of sudden cardiac death due to arrhythmias.The present narrative review aims to analyze the effects of MedD on CVD

Reverse Engineering Gene Networks with ANN: Variability in Network Inference Algorithms

Motivation :Reconstructing the topology of a gene regulatory network is one of the key tasks in systems biology. Despite of the wide variety of proposed methods, very little work has been dedicated to the assessment of their stability properties. Here we present a methodical comparison of the performance of a novel method (RegnANN) for gene network inference based on multilayer perceptrons with three reference algorithms (ARACNE, CLR, KELLER), focussing our analysis on the prediction variability induced by both the network intrinsic structure and the available data. Results: The extensive evaluation on both synthetic data and a selection of gene modules of "Escherichia coli" indicates that all the algorithms suffer of instability and variability issues with regards to the reconstruction of the topology of the network. This instability makes objectively very hard the task of establishing which method performs best. Nevertheless, RegnANN shows MCC scores that compare very favorably with all the other inference methods tested. Availability: The software for the RegnANN inference algorithm is distributed under GPL3 and it is available at the corresponding author home page (http://mpba.fbk.eu/grimaldi/regnann-supmat

Atmospheric production of energetic protons, electrons and positrons observed in near Earth orbit

Abstract Substantial fluxes of protons and leptons with energies below the geomagnetic cutoff have been measured by the AMS experiment at altitudes of 350–390 km, in the latitude interval ±51.7°. The production mechanisms of the observed trapped fluxes are investigated in detail by means of the FLUKA Monte Carlo simulation code. All known processes involved in the interaction of the cosmic rays with the atmosphere (detailed descriptions of the magnetic field and the atmospheric density, as well as the electromagnetic and nuclear interaction processes) are included in the simulation. The results are presented and compared with experimental data, indicating good agreement with the observed fluxes. The impact of the secondary proton flux on particle production in atmosphere is briefly discussed

Quantum ESPRESSO: a modular and open-source software project for quantum simulations of materials

Quantum ESPRESSO is an integrated suite of computer codes for electronic-structure calculations and materials modeling, based on density-functional theory, plane waves, and pseudopotentials (norm-conserving, ultrasoft, and projector-augmented wave). Quantum ESPRESSO stands for "opEn Source Package for Research in Electronic Structure, Simulation, and Optimization". It is freely available to researchers around the world under the terms of the GNU General Public License. Quantum ESPRESSO builds upon newly-restructured electronic-structure codes that have been developed and tested by some of the original authors of novel electronic-structure algorithms and applied in the last twenty years by some of the leading materials modeling groups worldwide. Innovation and efficiency are still its main focus, with special attention paid to massively-parallel architectures, and a great effort being devoted to user friendliness. Quantum ESPRESSO is evolving towards a distribution of independent and inter-operable codes in the spirit of an open-source project, where researchers active in the field of electronic-structure calculations are encouraged to participate in the project by contributing their own codes or by implementing their own ideas into existing codes.Comment: 36 pages, 5 figures, resubmitted to J.Phys.: Condens. Matte

Years of life that could be saved from prevention of hepatocellular carcinoma

BACKGROUND: Hepatocellular carcinoma (HCC) causes premature death and loss of life expectancy worldwide. Its primary and secondary prevention can result in a significant number of years of life saved. AIM: To assess how many years of life are lost after HCC diagnosis. METHODS: Data from 5346 patients with first HCC diagnosis were used to estimate lifespan and number of years of life lost after tumour onset, using a semi-parametric extrapolation having as reference an age-, sex- and year-of-onset-matched population derived from national life tables. RESULTS: Between 1986 and 2014, HCC lead to an average of 11.5 years-of-life lost for each patient. The youngest age-quartile group (18-61 years) had the highest number of years-of-life lost, representing approximately 41% of the overall benefit obtainable from prevention. Advancements in HCC management have progressively reduced the number of years-of-life lost from 12.6 years in 1986-1999, to 10.7 in 2000-2006 and 7.4 years in 2007-2014. Currently, an HCC diagnosis when a single tumour <2 cm results in 3.7 years-of-life lost while the diagnosis when a single tumour 65 2 cm or 2/3 nodules still within the Milan criteria, results in 5.0 years-of-life lost, representing the loss of only approximately 5.5% and 7.2%, respectively, of the entire lifespan from birth. CONCLUSIONS: Hepatocellular carcinoma occurrence results in the loss of a considerable number of years-of-life, especially for younger patients. In recent years, the increased possibility of effectively treating this tumour has improved life expectancy, thus reducing years-of-life lost