35 research outputs found
Quantum-Enhanced Diamond Molecular Tension Microscopy for Quantifying Cellular Forces
The constant interplay and information exchange between cells and their
micro-environment are essential to their survival and ability to execute
biological functions. To date, a few leading technologies such as traction
force microscopy, have been broadly used in measuring cellular forces. However,
the considerable limitations, regarding the sensitivity and ambiguities in data
interpretation, are hindering our thorough understanding of mechanobiology.
Herein, we propose an innovative approach, namely quantum-enhanced diamond
molecular tension microscopy (QDMTM), to precisely quantify the integrin-based
cell adhesive forces. Specifically, we construct a force sensing platform by
conjugating the magnetic nanotags labeled, force-responsive polymer to the
surface of diamond membrane containing nitrogen vacancy (NV) centers. Thus, the
coupled mechanical information can be quantified through optical readout of
spin relaxation of NV centers modulated by those magnetic nanotags. To validate
QDMTM, we have carefully performed corresponding measurements both in control
and real cell samples. Particularly, we have obtained the quantitative cellular
adhesion force mapping by correlating the measurement with established
theoretical model. We anticipate that our method can be routinely used in
studying important issues like cell-cell or cell-material interactions and
mechanotransduction.Comment: 51 pages, 20 figure