Methodological developments for the chemical synthesis of proteins and analogs

National audienc

Naturally-occuring disulfide rich antimicrobial peptides: Their chemical production for structural and functionnal studies

International audienc

Synthesis and structural characterization of naturally-occuring disulfide rich antimicrobial peptides Gyros Protein Technologies

International audienc

Solid phase chemical ligation

International audienc

Synthesis of N-Hnb-Cys peptide crypto-thioesters: identification of imadozolidinone and epimerized side products, and development of an optimized protocol

International audienc

Location and base selectivity on fragmentation of brominated oligodeoxynucleotides

Bromine-modified oligodeoxynucleotides (ODNs) were fragmented in the electrospray source to study the influence of brominated bases on fragmentation. Several 13-mer ODNs containing a brominated pyrimidine base, BrdU (5-bromodeoxyuridine) or BrdC (5-bromodeoxycytidine), were analyzed. Low cone voltage fragmentation yields a loss of the brominated base with a preferential loss for the brominated base closer to the 5-end (2-position > 4-position > 12-position) as well as a preferential loss of BrdU over BrdC. Higher cone voltage produces backbone fragmentation with complementary an-base and wm ions close to the brominated base. On the basis of these observations, we located the brominated base in the sequence for all of the ODNs studied

Design and optimization of alternative thioester precursors to simplify protein synthesis by native chemical ligation

International audienc

N-2-Hydroxybenzyl-Cysteine Peptides as Efficient Thioester Surrogates for Native Chemical Ligation

International audienc

Second generation N-2-hydroxybenzyl-cysteine peptide crypto-thioesters for native chemical ligation

International audienc