Etude génétique, chez E.Coli d'un mécanisme de réparation inductible et mutagène

Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe

Cancer et mécanismes de réparation de l'ADN (approche expérimentale par l'étude de partenaires protéiques de Rad 51, protéine de la réparation par recombinaison homologue)

BORDEAUX2-BU Santé (330632101) / SudocSudocFranceF

RAD51 et la tubuline-gamma (corrélation entre réparation par recombinaison homologue et établissement du fuseau mitotique)

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

UV reactivation of phage λ in a polA mutant of E. coli

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Kinetics of induction of error prone repair of bacteriophage λ by temperature shift in an Escherichia coli dnaB mutant

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Phage yield during W-reactivation of bacteriophage λ

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Etude des réponses de cellules de mammifères aux agents pontant l'ADN (identification de gènes impliqués dans le sensibilité à la mitomycine C)

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Resistance to the antibiotic Zeocin by stable expression of the Sh ble gene does not fully suppress Zeocin-induced DNA cleavage in human cells.

Zeocin is a member of the bleomycin/phleomycin family of antibiotics, known to bind and cleave DNA. We established human SK-OV-3 cells that stably express the Zeocin resistance gene (Sh ble) using an ecdysone-inducible mammalian expression system. Surprisingly, our results demonstrated that Zeocin, added in the culture medium to maintain the expression of the ecdysone receptor, was responsible for the formation of DNA strand breaks in the recombinant cells. This suggests that the Zeocin is not completely detoxified and is still able to cleave DNA, despite the stable expression of the Sh ble gene in the recombinant clones. Our study indicates that one needs to be very cautious in the interpretation of data involving stable cell lines selected with Zeocin

Molecular mechanisms of induced mutagenesis. Replication in vivo of bacteriophage phiX174 single-stranded, ultraviolet light-irradiated DNA in intact and irradiated host cells

SCOPUS: ar.jinfo:eu-repo/semantics/publishe