Tratamiento de regeneración endodóntica en pulpa vital y necrótica, utilizando fibrina rica en plaquetas y Biodentine: reporte de caso

Endodontic regeneration is an inductive and reparative biological treatment indicated to replace cell damage at the level of the dentinopulp complex. The objective of this study is to report a case of Endodontic Regenerative Therapy, in young permanent first molars, in a 10-year-old patient, using platelet-rich fibrin and Biodentine, applying two different protocols for combined diagnoses of irreversible pulpitis and pulp necrosis. The patient attended consultation due to extensive carious lesions. Clinical and imaging evaluations (X-rays and Cone Beam) were performed. The protocol in the necrotic canal consisted of disinfection with a bi-antibiotic paste and placement of a second-generation autologous scaffold matrix, made of biodegradable platelet-rich fibrin which is also , biocompatible and therefore without risk of immune rejection. The protocol in the canals with irreversible pulpitis consisted in a complete pulpotomy and placement of Biodentine calcium silicate cement, a material considered as first choice for pulp-conserving treatments, with properties similar to dentin. Eight weeks after the end of the treatment, a clinical control was conducted revealing an asymptomatic chart and negative palpation without the presence of fistula or abscess. This result indicated regeneration of the pulp tissues and success of the applied protocols. Controls will be carried out at 6, 12 and 18 months. Conclusion: The application of endodontic regenerative treatments, as performed in this reported case, is a favorable and innovative option to preserve, restore or replace the dental pulp. In comparison with conventional treatments, this procedure allows to return pulp functionality, complete development of the root, thickening of the dentinal walls and closure of the apical foramen of young permanent teeth affected by pulp pathologies. It is important that regenerative therapies are publicized and applied by dental professionalsLa regeneración endodóntica es un tratamiento inductivo y reparativo con bases biológicas, indicado para reemplazar los daños celulares a nivel del complejo dentinopulpar. El objetivo de este estudio es reportar un caso de Terapia Regenerativa Endodóntica, en primeros molares permanentes jóvenes, en un paciente de 10 años, a través del uso de fibrina rica en plaquetas y Biodentine, se aplicaron dos protocolos diferentes por diagnósticos combinados de pulpitis irreversible y necrosis pulpar. El paciente acude a consulta por presentar lesiones cariosas extensas; se realizaron evaluaciones clínicas e imagenológicas (radiografías y Cone Beam), el protocolo en el conducto necrótico consistió en la desinfección con pasta biantibiótica y la colocación de una matriz de andamiaje autóloga de segunda generación, elaborada de fibrina rica en plaquetas que es biodegradable, biocompatible y no existe riesgo de rechazo inmunológico. El protocolo en los conductos con pulpitis irreversibles consistió en pulpotomía total y colocación de cemento de silicato de calcio Biodentine, el cual se considera el material de primera elección para los tratamientos conservadores de la pulpa, con propiedades similares a la dentina. A 8 semanas de finalizado el tratamiento se realizó control clínico y el paciente mostró un cuadro asintomático, palpación negativa sin presencia de fístula o absceso lo que podría indicar regeneración de los tejidos pulpares y éxito de los protocolos aplicados. Los siguientes controles se realizán a los 6, 12 y 18 meses. Conclusión: la aplicación de los tratamientos regenerativos endodónticos como se realizó en el caso reportado, es una opción favorable e innovadora para preservar, restaurar o sustituir la pulpa dental, que en comparación con los tratamientos convencionales nos permiten devolver la funcionalidad pulpar, finalizar el desarrollo radicular, engrosamiento de las paredes dentinales y cierre del foramen apical de dientes permanentes jóvenes que han sido afectados por patologías pulpares. Es importante que las terapias regenerativas sean divulgadas y aplicadas por los profesionale

Pion electro-production in the Roper region in chiral quark models

We present a method to calculate pion electro-production amplitudes in a coupled-channel framework incorporating quasi-bound quark-model states. The method offers a clear prescription how to extract the resonant part of the amplitudes, even in the presence of different decay channels and a strong mixing of neighbouring resonances. The method is applied to the calculation of the M1- and the S1- amplitudes in the P11 partial wave in a simple chiral quark model. A good agreement with the observed M1- amplitude is found with a significant contribution from the pion cloud. The same effect is also prominent in the S1- amplitude but a rather uncertain data prevent us to draw a definitive conclusion.Comment: 8 pages, 10 Postscript figures, Fig. 7 and Fig. 8 change

The syndrome of central hypothyroidism and macroorchidism: IGSF1 controls TRHR and FSHB expression by differential modulation of pituitary TGFβ and Activin pathways

IGSF1 (Immunoglobulin Superfamily 1) gene defects cause central hypothyroidism and macroorchidism. However, the pathogenic mechanisms of the disease remain unclear. Based on a patient with a full deletion of IGSF1 clinically followed from neonate to adulthood, we investigated a common pituitary origin for hypothyroidism and macroorchidism, and the role of IGSF1 as regulator of pituitary hormone secretion. The patient showed congenital central hypothyroidism with reduced TSH biopotency, over-secretion of FSH at neonatal minipuberty and macroorchidism from 3 years of age. His markedly elevated inhibin B was unable to inhibit FSH secretion, indicating a status of pituitary inhibin B resistance. We show here that IGSF1 is expressed both in thyrotropes and gonadotropes of the pituitary and in Leydig and germ cells in the testes, but at very low levels in Sertoli cells. Furthermore, IGSF1 stimulates transcription of the thyrotropin-releasing hormone receptor (TRHR) by negative modulation of the TGFβ1-Smad signaling pathway, and enhances the synthesis and biopotency of TSH, the hormone secreted by thyrotropes. By contrast, IGSF1 strongly down-regulates the activin-Smad pathway, leading to reduced expression of FSHB, the hormone secreted by gonadotropes. In conclusion, two relevant molecular mechanisms linked to central hypothyroidism and macroorchidism in IGSF1 deficiency are identified, revealing IGSF1 as an important regulator of TGFβ/Activin pathways in the pituitary

Habilidades para la vida e inteligencia social como elementos favorecedores de la salud mental en universitarios

7 páginasThe purpose of this study was to analyze the relationship between life skills and social intelligence in students from a private university. It was developed under a quantitative approach, with a non-experimental design, descriptive-correlational, and cross-sectional. The population was made up of 506 university students enrolled in the first semesters (1, 2, and 3) of a private university. The sample was non-probabilistic and was made up of 278 students. The life skills test and the short social intelligence scale were used as instruments. To analyze the relationship between the variables studied, the Spearman-Brown correlation was used (since the data do not follow a normal distribution (Kolmogorov- Smirnov; P 0.05), the relationship between the variables studied (life skills and social intelligence), according to the Spearman correlation -Brown, it was found that there is a relationship between the dimensions of the variables studied (P<0.05), in the same way, it was also found that said relationship is directly proportional. Life and social intelligence are necessary variables in the processes of academic training, and according to the results obtained, it is considered pertinent to develop training and intervention actions in them, to favor adaptation to the university context, student permanence, and scope of graduate professional competencies. © 2021 Academia Nacional de Medicina. All rights reserved

Mudanças climáticas e impactos na necessidade hídrica das culturas perenes na Bacia do Jaguaribe, no Estado do Ceará Climate change and impacts on water requirement of permanent crops in the Jaguaribe Basin, Ceará, Brazil

O objetivo deste trabalho foi avaliar os impactos das mudanças climáticas na demanda de água para irrigação de culturas perenes, na Bacia do Jaguaribe, no Estado do Ceará. Foi empregado o sistema integrado de modelagem regional PRECIS ("Providing Regional Climates for Impact Studies"), e aplicado o método de redução de escala de bacia hidrográfica, com as condições de contorno do modelo climático regional (HadRM3P). Foi utilizado um conjunto de climatologia de base do modelo de 1961 a 1990 e de projeções climáticas futuras. As coordenadas geográficas da região em estudo foram consideradas para interpolação num sistema de informação geográfica. A evapotranspiração de referência foi estimada por meio de dados da temperatura média mensal. As mudanças climáticas projetadas aumentaram a demanda projetada de água para irrigação, porque a evapotranspiração foi estimada para aumentos de 3,1 a 2,2% e a precipitação pluvial foi estimada para diminuições de 30,9 a 37,3%. O aumento da necessidade hídrica foi estimada em 32,9% a 43,9%, para o ano de 2040, conforme o cenário analisado.<br>The aim of this study was to estimate climate change impacts on irrigation water demand for permanent crops. The PRECIS (Providing Regional Climates for Impact Studies) system was applied, and downscaling techniques were used at the river basin level, with the boundary conditions of the regional climate model (HadRM3P). A climate data set was generated for 1961 to 1990 (baseline) and for future climate projections. The regional geographical coordinates were considered for interpolation in a georeferenced coordinated system. The reference evapotranspiration was estimated through data of monthly average temperature. Projected climate change increased projected irrigation water demand, because evapotranspiration was estimated to increase by 3.1 to 2.2% and rainfall was estimated to decrease by 30.9 to 37.3%. The 2040 water need was estimated to increase by 32.9% to 43.9%, according to the analyzed scenario

Isolation and molecular characterization of Xylella fastidiosa from coffee plants in Costa Rica.

Copyright The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2008. / . Datos incluidos por Lisela Moreira Carmona.Coffee plants exhibiting a range of symptoms including mild to severe curling of leaf margins, chlorosis and deformation of leaves, stunting of plants, shortening of internodes, and dieback of branches have been reported since 1995 in several regions of Costa Rica's Central Valley. The symptoms are referred to by coffee producers in Costa Rica as "crespera" disease and have been associated with the presence of the bacterium Xylella fastidiosa. Coffee plants determined to be infected by the bacterium by enzyme linked immunosorbent assay (ELISA), were used for both transmission electron microscopy (TEM) and for isolation of the bacterium in PW broth or agar. Petioles examined by TEM contained rod-shaped bacteria inside the xylem vessels. The bacteria measured 0.3 to 0.5 μm in width and 1.5 to 3.0 μm in length, and had rippled cell walls 10 to 40 nm in thickness, typical of X. fastidiosa. Small, circular, dome-shaped colonies were observed 7 to 26 days after plating of plant extracts on PW agar. The colonies were comprised of Gram-negative rods of variable length and a characteristic slight longitudinal bending. TEM of the isolated bacteria showed characteristic rippled cell walls, similar to those observed in plant tissue. ELISA and PCR with specific primer pairs 272-l-int/272-2-int and RST31/RST33 confirmed the identity of the isolated bacteria as X. fastidiosa. RFLP analysis of the amplification products revealed diversity within X. fastidiosa strains from Costa Rica and suggest closer genetic proximity to strains from the United States of America than to other coffee or citrus strains from Brazil.Fundación CR-USAUniversidad de Costa Rica/[801-A2-528]/UCR/Costa RicaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM