A replicated climate change field experiment reveals rapid evolutionary response in an ecologically important soil invertebrate

AGAP : GE2pop - Génomique évolutive et gestion des populationsInternational audienceWhether species can respond evolutionarily to current climate change is crucial for the persistence of many species. Yet, very few studies have examined genetic responses to climate change in manipulated experiments carried out in natural field conditions. We examined the evolutionary response to climate change in a common annelid worm using a controlled replicated experiment where climatic conditions were manipulated in a natural setting. Analyzing the transcribed genome of 15 local populations, we found that about 12% of the genetic polymorphisms exhibit differences in allele frequencies associated to changes in soil temperature and soil moisture. This shows an evolutionary response to realistic climate change happening over short-time scale, and calls for incorporating evolution into models predicting future response of species to climate change. It also shows that designed climate change experiments coupled with genome sequencing offer great potential to test for the occurrence (or lack) of an evolutionary response

COX-I multiple alignment for barcoding

Alignment of the Cox-1 reference sequence we used to barcode our samples– labelled JN259763 – with a set of Cox-1 sequences published more recently: http://datadryad.org/resource/doi:10.5061/dryad.6mh2

Coaching in the game of Squash

Title: Coaching in the game of Squash Aim of thesis: - To understand how Czech trainers approach a problem of coaching of their trainees. - Whether they also step in as coaches or not. - Whether they underestimate a psychological preparation of players. - If players are content with the approach and coaching by their trainers. - Whether a non-verbal communication is used in coaching. Methods: A questionnaire among players and trainers active in the Czech Republic. Results were collected through a questionnaire that was either sent by email or handed out to a predetermined group of respondents. The survey took place in the whole Czech Republic. The respondents were both male and female squash players from all parts of the republic. Results: - In most cases the trainers also step in as coaches. - Trainers are well aware of the importance of psychological preparation but share a common feeling that their knowledge is insufficient. They work diligently on improvement. - Not very often do trainers accompany trainees to tournaments, especially to the extent that would benefit the trainees. - In roughly half of all cases, players express a high level of satisfaction with the coaching style of their trainers. The rest finds the way information is passed onto them somewhat flawed. - Female trainers better..

Filtered Contigs

File (FASTA format) containing the sequence of all contigs obtained from de novo assembly of transcriptome sequencing. Only contigs of at least 200 nt and having average coverage of 20x are included

Raw counts of minor allele frequency at each SNP position (5' UTR)

CSV file with counts of reads supporting each allele at each SNP position and in each populatio