Timing of alveolar cleft bone grafting in maxillary alveolar cleft defects

Numerous methods have been attempted to identify the best time for secondary alveolar cleft bone grafting, including chronological age, skeletal age, and dental age. However, few studies have employed objective methods of assessment that would permit statistical analysis. Fifty-nine patients with clefts of the alveolus who acquired secondary alveolar cleft grafts at the Lancaster Cleft Lip and Palate Clinic were studied. A total of 74 affected areas from 15 bilateral and 44 unilateral alveolar cleft patients were available. Timing of the graft was determined utilizing root development of the involved canine, as compared to crown length, from a high quality pre-graft radiograph taken no more than six weeks prior to surgery. A Post-graph radiograph exposed approximately 2 years post-surgery was digitized to assess the final bony architecture

Wind-Driven, Near-Bottom Currents Over the West Louisiana Inner Continental Shelf.

Forcing mechanisms and water column response over the West Louisiana Inner Continental Shelf (WLICS) are investigated at various temporal and spatial scales. The major mechanisms that have an effect on shallow-water currents over the WLICS are winds, runoff from the Mississippi and Atchafalaya rivers, and circulation of the northwestern Gulf of Mexico. Summer wind stress values are low and currents generally meander over the inner shelf during this period. A shift in the direction of the regional wind stress in late June causes a reversal in the current direction over the west Louisiana shelf. Autumn currents are primarily westward in response to predominantly westward wind stress. Winter currents are primarily westward, but during frontal passages the current swings rapidly to the east. As winds become easterly, the currents return to their westward set. During the spring the flood on the Mississippi and Atchafalaya rivers introduces a large amount of fresh water into the coastal waters. The fresh water causes density gradients, which decouple near-bottom flow from surface winds and increase the intensity of the westward currents. During the autumn and winter regional-scale cyclonic flow, generated by a succession of cold-front passages, helps to maintain flow over the WLICS in a westward direction. Four periods of frontal passage in 1979 are studied in detail. Strong alongshelf wind stress is common during the January and March frontal periods. An investigation of water column dynamics reveals that the alongshelf wind stress accelerates the water column and generates a frictional boundary layer at the bottom. A strong high pressure system pushes a front across the WLICS from the north during February. Dynamically, low alongshelf wind stress accelerates the water column, but it is not strong enough to establish a frictional balance. An alongshelf pressure gradient also influences the water column dynamics. South-southeasterly winds and freshwater runoff dominate the April frontal period. The alongshelf wind stress accelerates the water column, but no frictional balance is established. A pressure gradient term due to the Atchafalaya flood contributes to the momentum balance

Analyzing Satellite Ocean Color Match-Up Protocols Using the Satellite Validation Navy Tool (SAVANT) At MOBY and Two AERONET-OC Sites

The satellite validation navy tool (SAVANT) was developed by the Naval Research Laboratory to help facilitate the assessment of the stability and accuracy of ocean color satellites, using numerous ground truth (in situ) platforms around the globe and support methods for match-up protocols. The effects of varying spatial constraints with permissive and strict protocols on match-up uncertainty are evaluated, in an attempt to establish an optimal satellite ocean color calibration and validation (cal/val) match-up protocol. This allows users to evaluate the accuracy of ocean color sensors compared to specific ground truth sites that provide continuous data. Various match-up constraints may be adjusted, allowing for varied evaluations of their effects on match-up data. The results include the following: (a) the difference between aerosol robotic network ocean color (AERONET-OC) and marine optical Buoy (MOBY) evaluations; (b) the differences across the visible spectrum for various water types; (c) spatial differences and the size of satellite area chosen for comparison; and (d) temporal differences in optically complex water. The match-up uncertainty analysis was performed using Suomi National Polar-orbiting Partnership (SNPP) Visible Infrared Imaging Radiometer Suite (VIIRS) SNPP data at the AERONET-OC sites and the MOBY site. It was found that the more permissive constraint sets allow for a higher number of match-ups and a more comprehensive representation of the conditions, while the restrictive constraints provide better statistical match-ups between in situ and satellite sensors

Periodontal Status and Quality of Life: Impact of Fear of Pain and Dental Fear

Background. Oral health-related quality of life (OHRQoL) is impacted by periodontal disease and orofacial pain. There is a limited research examining the impact of avoidance of care or physiological arousal related to the fear of pain response on periodontal-related OHRQoL. Methods. Data are from the Center for Oral Health Research in Appalachia family-based study focusing on 1,339 adults. Measures included a modified Periodontal Screening and Recording Index across sextants of dentition, dental fear survey, Fear of Pain Questionnaire-9, and Oral Health Impact Profile-14. Structural equation modeling was used to estimate the effects of periodontal disease screening indicators on OHRQoL including the mediating role of dental fear while accounting for fear of pain. Results. A significant total effect was found for the mandibular anterior sextant, components of dental anxiety/fear, and indicators of OHRQoL (pain and discomfort, , ; psychosocial impact, , ). The maxillary anterior region was significantly associated with pain discomfort (, ) and functionality (, ). Conclusions. Findings provide a granular perspective of periodontal disease indicators and OHRQoL. Dental avoidance/anticipatory fear and physiological arousal mediate OHRQoL in individuals who have indicators of periodontal disease in sextants that may be visible and susceptible to higher pain and psychosocial impact

Human Telomere Length Correlates to the Size of the Associated Chromosome Arm

The majority of human telomere length studies have focused on the overall length of telomeres within a cell. In fact, very few studies have examined telomere length for individual chromosome arms. The objective of this study was to examine the relationship between chromosome arm size and the relative length of the associated telomere. Quantitative Fluorescence In Situ Hybridization (Q-FISH) was used to measure the relative telomere length of each chromosome arm in metaphases from cultured lymphocytes of 17 individuals. A statistically significant positive correlation (r = 0.6) was found between telomere length and the size of the associated chromosome arm, which was estimated based on megabase pair measurements from http://www.ncbi.nlm.nih.gov/projects/mapview/

Cryptic Subtelomeric Rearrangements and X Chromosome Mosaicism: A Study of 565 Apparently Normal Individuals with Fluorescent In Situ Hybridization

Five percent of patients with unexplained mental retardation have been attributed to cryptic unbalanced subtelomeric rearrangements. Half of these affected individuals have inherited the rearrangement from a parent who is a carrier for a balanced translocation. However, the frequency of carriers for cryptic balanced translocations is unknown. To determine this frequency, 565 phenotypically normal unrelated individuals were examined for balanced subtelomeric rearrangements using Fluorescent In Situ hybridization (FISH) probes for all subtelomere regions. While no balanced subtelomeric rearrangements were identified, three females in this study were determined to be mosaic for the X chromosome. Mosaicism for XXX cell lines were observed in the lymphocyte cultures of 3 in 379 women (0.8%), which is a higher frequency than the 1 in 1000 (0.1%) reported for sex chromosome aneuploidies. Our findings suggest that numerical abnormalities of the X chromosome are more common in females than previously reported. Based on a review of the literature, the incidence of cryptic translocation carriers is estimated to be approximately 1/8,000, more than ten-fold higher than the frequency of visible reciprocal translocations

High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia

BACKGROUND: To investigate the potential association between oral health and cognitive function, a pilot study was conducted to evaluate high throughput DNA sequencing of the V3 region of the 16S ribosomal RNA gene for determining the relative abundance of bacterial taxa in subgingival plaque from older adults with or without dementia. METHODS: Subgingival plaque samples were obtained from ten individuals at least 70 years old who participated in a study to assess oral health and cognitive function. DNA was isolated from the samples and a gene segment from the V3 portion of the 16S bacterial ribosomal RNA gene was amplified and sequenced using an Illumina HiSeq1000 DNA sequencer. Bacterial populations found in the subgingival plaque were identified and assessed with respect to the cognitive status and oral health of the participants who provided the samples. RESULTS: More than two million high quality DNA sequences were obtained from each sample. Individuals differed greatly in the mix of phylotypes, but different sites from different subgingival depths in the same subject were usually similar. No consistent differences were observed in this small sample between subjects separated by levels of oral health, sex, or age; however a consistently higher level of Fusobacteriaceae and a generally lower level of Prevotellaceae was seen in subjects without dementia, although the difference did not reach statistical significance, possibly because of the small sample size. CONCLUSIONS: The results from this pilot study provide suggestive evidence that alterations in the subgingival microbiome are associated with changes in cognitive function, and provide support for an expanded analysis of the role of the oral microbiome in dementia

Effects of Smoking and Genotype on the PSR Index of Periodontal Disease in Adults Aged 18–49

Studies have found both genetic and environmental influences on chronic periodontitis. The purpose of this study was to examine the relationships among previously identified genetic variants, smoking status, and two periodontal disease-related phenotypes (PSR1 and PSR2) in 625 Caucasian adults (aged 18–49 years). The PSR Index was used to classify participants as affected or unaffected under the PSR1 and PSR2 phenotype definitions. Using logistic regression, we found that the form of the relationship varied by single nucleotide polymorphism (SNP): For rs10457525 and rs12630931, the effects of smoking and genotype on risk were additive; whereas for rs10457526 and rs733048, smoking was not independently associated with affected status once genotype was taken into consideration. In contrast, smoking moderated the relationships of rs3870371 and rs733048 with affected status such that former and never smokers with select genotypes were at increased genetic risk. Thus, for several groups, knowledge of genotype may refine the risk prediction over that which can be determined by knowledge of smoking status alone. Future studies should replicate these findings. These findings provide the foundation for the exploration of novel pathways by which periodontitis may occur

A Preliminary Genome-Wide Association Study of Pain-Related Fear: Implications for Orofacial Pain

Background. Acute and chronic orofacial pain can significantly impact overall health and functioning. Associations between fear of pain and the experience of orofacial pain are well-documented, and environmental, behavioral, and cognitive components of fear of pain have been elucidated. Little is known, however, regarding the specific genes contributing to fear of pain. Methods. A genome-wide association study (GWAS; ) was performed to identify plausible genes that may predispose individuals to various levels of fear of pain. The total score and three subscales (fear of minor, severe, and medical/dental pain) of the Fear of Pain Questionnaire-9 (FPQ-9) were modeled in a variance components modeling framework to test for genetic association with 8.5 M genetic variants across the genome, while adjusting for sex, age, education, and income. Results. Three genetic loci were significantly associated with fear of minor pain (8q24.13, 8p21.2, and 6q26; for all) near the genes TMEM65, NEFM, NEFL, AGPAT4, and PARK2. Other suggestive loci were found for the fear of pain total score and each of the FPQ-9 subscales. Conclusions. Multiple genes were identified as possible candidates contributing to fear of pain. The findings may have implications for understanding and treating chronic orofacial pain

Genome-Wide Association Study of Periodontal Health Measured by Probing Depth in Adults Ages 18−49 years

The etiology of chronic periodontitis clearly includes a heritable component. Our purpose was to perform a small exploratory genome-wide association study in adults ages 18–49 years to nominate genes associated with periodontal disease−related phenotypes for future consideration. Full-mouth periodontal pocket depth probing was performed on participants (N = 673), with affected status defined as two or more sextants with probing depths of 5.5 mm or greater. Two variations of this phenotype that differed in how missing teeth were treated were used in analysis. More than 1.2 million genetic markers across the genome were genotyped or imputed and tested for genetic association. We identified ten suggestive loci (p-value ≤ 1E-5), including genes/loci that have been previously implicated in chronic periodontitis: LAMA2, HAS2, CDH2, ESR1, and the genomic region on chromosome 14q21-22 between SOS2 and NIN. Moreover, we nominated novel loci not previously implicated in chronic periodontitis or related pathways, including the regions 3p22 near OSBPL10 (a lipid receptor implicated in hyperlipidemia), 4p15 near HSP90AB2P (a heat shock pseudogene), 11p15 near GVINP1 (a GTPase pseudogene), 14q31 near SEL1L (an intracellular transporter), and 18q12 in FHOD3 (an actin cytoskeleton regulator). Replication of these results in additional samples is needed. This is one of the first research efforts to identify genetic polymorphisms associated with chronic periodontitis-related phenotypes by the genome-wide association study approach. Though small, efforts such this are needed in order to nominate novel genes and generate new hypotheses for exploration and testing in future studies