Integration As A Competitiveness Instrument For Public Transport In Rural Areas

The market of interurban mobility to rural areas is one of the most challenging for Public Transport (PT). In Portugal it has been quite difficult for Public Transportation to establish itself in this market as an effective alternative to the use of private cars. Two of the main reasons for this are the low accessibility of long distance railway services and urban sprawl in many Portuguese regions. Integration facilitates the use of several services of the transport chain and facilitates a more efficient resource allocation on the supply side of the system. By making local distribution possible in connection with long distance services, even in low demand areas, integration may foster a higher patronage of PT and its competitiveness compared to private car. This paper presents the study of five short-range road transport services in connection to the railway network in the Médio Tejo region, including the key elements that are to be considered on their design. The research identified the possible interested agents and made an account of their related costs and benefits. The conclusion is that these services can be commercially interesting and may bring considerable increase in the market share of PT. Keywords: Integration, Multimodal Transport, Public Transport, Interurban Transport, Economic Evaluation.Institute of Transport and Logistics Studies. Faculty of Economics and Business. The University of Sydne

Integration As A Competitiveness Instrument For Public Transport In Rural Areas

The market of interurban mobility to rural areas is one of the most challenging for Public Transport (PT). In Portugal it has been quite difficult for Public Transportation to establish itself in this market as an effective alternative to the use of private cars. Two of the main reasons for this are the low accessibility of long distance railway services and urban sprawl in many Portuguese regions. Integration facilitates the use of several services of the transport chain and facilitates a more efficient resource allocation on the supply side of the system. By making local distribution possible in connection with long distance services, even in low demand areas, integration may foster a higher patronage of PT and its competitiveness compared to private car. This paper presents the study of five short-range road transport services in connection to the railway network in the Médio Tejo region, including the key elements that are to be considered on their design. The research identified the possible interested agents and made an account of their related costs and benefits. The conclusion is that these services can be commercially interesting and may bring considerable increase in the market share of PT. Keywords: Integration, Multimodal Transport, Public Transport, Interurban Transport, Economic Evaluation.Institute of Transport and Logistics Studies. Faculty of Economics and Business. The University of Sydne

Ethnozoological Impacts on Fauna Conservation: The Case of Chimanimani National Park

Interactions between humans and fauna have been considered essential to better understand the dynamics of socio-ecological systems. This study aimed to assess the ethno-zoological impact on fauna conservation in Chimanimani National Park, by studying the Mpunga Centro community values applied in the use of fauna species, their participation in conservation and the mensuration of ethnozoological impacts. Methodologically, bibliographic review was used for theoretical support and the data collection in the field for information analysis in a sample of 82 households. The collected data were processed on SPSS statistical package, version 20.0 and analyzed at a level of significance of 5%. The results showed that the community ecologically valorizes the species, on traditional medicine, on educational and recreational, cultural and religious value.  The results also showed that the community participates in conservation through preventing uncontrolled fires, getting involved in conservation agriculture, beekeeping, community inspection and ecotourism. Having concluded that the traditional knowledge of Mpunga Centro Community on the use of local fauna, influences on wildlife conservation of the Chimanimani National Park. Keywords: Ethnozoology, conservation, local community, fauna species, Chimanimani National Park DOI: 10.7176/JRDM/76-02 Publication date:June 30th 202

Human seroprevalence of Toscana virus and Sicilian phlebovirus in the southwest of Portugal

Funding Information: Fundação para a Ciência e a Tecnologia, I.P. (FCT) for funding through contract GHTM-UID/Multi/04413/2013 and Investigator Starting Grant IF/01302/2015. AP was supported by the Portuguese Ministry of Education and Science (via FCT) through a PhD grant (SFRH/BD/116516/2016). Publisher Copyright: © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.Toscana virus (TOSV) is emergent in the Mediterranean region and responsible for outbreaks of encephalitis or meningoencephalitis. Sicilian phlebovirus (SFSV) cause epidemics of febrile illness during the summer. The aim of this study was to evaluate the presence of antibodies against TOSV and SFSV in humans in the southwest of Portugal. Neutralizing antibodies to TOSV and SFSV were respectively detected in 5.3% and 4.3% out of 400 human sera tested highlighting the need to increase public health awareness regarding phleboviruses and to include them in the differential diagnosis in patients presenting with fever of short duration and neurological manifestations.publishersversionpublishe

Improving kinetic or thermodynamic stability of an azoreductase by directed evolution

Protein stability arises from a combination of factors which are often difficult to rationalise. Therefore its improvement is better addressed through directed evolution than by rational design approaches. In this study, five rounds of mutagenesis/recombination followed by high-throughput screening (approximate to 10,000 clones) yielded the hit 1B6 showing a 300-fold higher half life at 50 degrees C than that exhibited by the homodimeric wild type PpAzoR azoreductase from Pseudomonas putida MET94. The characterization using fluorescence, calorimetry and light scattering shows that 1B6 has a folded state slightly less stable than the wild type (with lower melting and optimal temperatures) but in contrast is more resistant to irreversible denaturation. The superior kinetic stability of 1B6 variant was therefore related to an increased resistance of the unfolded monomers to aggregation through the introduction of mutations that disturbed hydrophobic patches and increased the surface net charge of the protein. Variants 2A1 and 2A1-Y179H with increased thermodynamic stability (10 to 20 degrees C higher melting temperature than wild type) were also examined showing the distinctive nature of mutations that lead to improved structural robustness: these occur in residues that are mostly involved in strengthening the solvent-exposed loops or the inter-dimer interactions of the folded state.European Union [BIORENEW,, FP6-2004-NMP-NI-4/026456]; Fundacao para a Ciencia e Tecnologia, Portugal [PEst-OE/EQB/LA0004/2011, PTDC/QUI-BIQ/119677/2010]; FCT, Portugal [SFRH/BPD/46808/2008

Circles and communities, sharing practices and learning: Looking at old and new extension education approaches

Abstract: We live today in a "knowledge society", but "knowledge transfer" is no more the dominant extension education paradigm. Th

Antibody response to Phlebotomus perniciosus saliva in cats naturally exposed to phlebotomine sand flies is positively associated with Leishmania infection

BACKGROUND: Zoonotic leishmaniosis, caused by the protozoan Leishmania infantum, is a public and animal health problem in Asia, Central and South America, the Middle East and the Mediterranean Basin. Several phlebotomine sand fly species from the subgenus Larroussius are vectors of L. infantum. Data from dogs living in endemic areas of leishmaniosis advocate the use of antibody response to phlebotomine sand fly saliva as an epidemiological biomarker for monitoring vector exposure. The aim of this study was to analyse the exposure of cats to phlebotomine sand flies using detection of IgG antibodies to Phlebotomus perniciosus saliva. The association between phlebotomine sand fly exposure and the presence of Leishmania infection was also investigated. RESULTS: IgG antibodies to P. perniciosus saliva were detected in 167 (47.7%) out of 350 cats; higher antibody levels were present in sera collected during the period of phlebotomine sand fly seasonal activity (OR = 19.44, 95% CI: 9.84-38.41). Cats of 12-35 months had higher antibody levels than younger ones (OR = 3.56, 95% CI: 1.39-9.16); this difference was also significant with older cats (for 36-95 months-old, OR = 9.43, 95% CI: 3.62-24.48; for older than 95 months, OR = 9.68, 95% CI: 3.92-23.91). Leishmania spp. DNA was detected in the blood of 24 (6.9%) cats, while antibodies to L. infantum were detected in three (0.9%). Only one cat was positive to Leishmania by both techniques. Cats presenting IgG antibodies to P. perniciosus had a significantly higher risk of being positive for Leishmania infection. CONCLUSIONS: To our knowledge, this is the first study demonstrating anti-sand fly saliva antibodies in cats. The evaluation of the contact of this animal species with the vector is important to the development of prophylactic measures directed to cats, with the aim of reducing the prevalence of infection in an endemic area. Therefore, studies evaluating whether the use of imidacloprid/flumethrin collars reduces the frequency of P. perniciosus bites in cats are needed. It is also important to evaluate if there is a correlation between the number of phlebotomine sand fly bites and IgG antibody levels.publishersversionpublishe

Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal

Funding Information: This paper has been sponsored by Elanco Animal Health in the framework of the CVBD®World Forum Symposium. The authors would like to acknowledge all the blood donors who agreed to participate in this study for contributing with their answers to the questionnaire and their blood samples. The authors would also like to acknowledge all the professionals of the following institutions that contributed to organization of physical equipment, presentation of the study to potential participants, obtaining of informed consent, separation and storage of samples and questionnaires: Instituto Português do Sangue e da Transplantação; Centro Hospitalar Universitário do Algarve; Hospital do Espírito Santo de Évora; Unidade Local de Sáude do Norte Alentejano; Unidade Local de Saúde do Baixo Alentejo and Unidade Local de Saúde do Litoral Alentejano. Lastly, the authors would like to acknowledge the researcher Regina Loesch for her contribution to filling in the electronic database with answers to the paper questionnaires, and the researcher Ana Rita Goes for her contributions to designing the questionnaire for this study. Funding Information: RR was supported by the Portuguese Ministry of Education and Science (via Fundação para a Ciência e a Tecnologia, I.P.—FCT) through a PhD grant (UI/BD/151067/2021). The authors would also like to acknowledge FCT for funding through contracts with GHTM (UID/Multi/04413/2020, UIDB/00006/2020 and UIDP/00006/2020) and LA-REAL (LA/P/0117/2020). This project was also funded by the Tropical Clinic Unit (Institute of Hygiene and Tropical Medicine [IHMT]). Publisher Copyright: © 2023, BioMed Central Ltd., part of Springer Nature.Background: Asymptomatic infection is the most common outcome of exposure to Leishmania parasites. In the Mediterranean region, where Leishmania infantum is endemic, studies on the prevalence of asymptomatic infection have often relied on serological testing in blood donors. In Spain, regional studies have shown seroprevalence in blood donors between 1 and 8%; in Portugal, values of 0 and 2% were suggested by two localized studies, in different populations. The purpose of this study was (i) to estimate the prevalence of asymptomatic Leishmania infection in blood donors in mainland Portugal, and (ii) to study the association between the detection of antibodies to Leishmania and sociodemographic factors, and also the knowledge, perceptions and practices (KPP) of the blood donors regarding leishmaniasis. Methods: A cross-sectional study targeted the population of people who donated blood in mainland Portugal. Participants, distributed proportionally by municipality and aged between 18 and 65 years, were selected randomly in 347 blood collection points between February and June 2022, and completed a sociodemographic and a KPP questionnaire. Detection of anti-Leishmania antibodies in serum was performed using an ELISA commercial kit. Individual KPP scores were calculated by adding grades defined for each question. Results: Globally, 201/3763 samples were positive. The estimated national true seroprevalence was 4.8% (95% CI 4.1–5.5%). The proportion of positive results was significantly different between NUTS (Nomenclature of Territorial Units for Statistics) regions. Models suggested that seropositivity was significantly higher in male sex, people older than 25 years, or residing in the Centro NUTS2 region, but not in dog owners nor people with lower KPP scores. Overall, 72.3% of participants had previously heard of leishmaniasis and, in multivariate analysis, a higher Knowledge score was associated with age 25–40 years, female sex, ownership of dogs, and higher education. Conclusions: Global estimated true seroprevalence (4.8%) was similar to previous regional studies in blood donors in neighboring Spain. Higher seroprevalence values in the NUTS2 Centro region were consistent with incidence data from humans and seroprevalence studies in dogs. On the other hand, the low values in the Alentejo and the high values in the northern subregions may be the result of geographical shifts in parasite circulation due to climate change and should prompt localized and integrated, vector, canine, and human research, following a One Health approach. Graphical Abstract: [Figure not available: see fulltext.].publishersversionpublishe

Anais do V Encontro de Escritores de Língua Portuguesa

Literatura e Lusofonia, colectânea dos textos apresentados ao V Encontro de Escritores de Língua Portuguesa. realizado na cidade de Luanda.Câmara Municipal de Luanda e Câmara Municipal de Lisboainfo:eu-repo/semantics/publishedVersio

Molecular detection of Leishmania infantum, filariae and Wolbachia spp. in dogs from southern Portugal

Background: Leishmaniosis caused by the protozoan Leishmania infantum and dirofilariosis caused by the nematodes Dirofilaria immitis or Dirofilaria repens are vector-borne zoonoses widely present in the Mediterranean basin. In addition, some studies reported that the endosymbiont Wolbachia spp. play a role in the biology and pathogenesis of filarial parasites. The aim of this work was to evaluate the frequency of mono-and co-infections by L. infantum, filariae and Wolbachia spp. and their association with clinical signs in dogs from the south of Portugal. Leishmanial, filarial and Wolbachia spp. DNA were evaluated by specific real-time polymerase chain reaction (qPCR) assays in blood samples from 230 dogs.Findings: One hundred and thirty-nine (60.4 %) dogs were qPCR-positive for L. infantum and 26 (11.3 %) for filariae (24 for D. immitis only, one D. immitis and for Acanthocheilonema dracunculoides and another one for Acanthocheilonema reconditum only). Wolbachia spp. DNA was amplified from 16 (64.0 %) out of the 25 D. immitis-positive dogs. Nineteen (8.3 %) dogs were co-infected with L. infantum and D. immitis, including the one (0.4 %) A. drancunculoides-positive animal. In dogs without clinical signs consistent with leishmaniosis and/or dirofilariosis, L. infantum prevalence was 69 %, whereas in those dogs with at least one clinical manifestation compatible with any of the two parasitoses prevalence was 42.7 %. Leishmania prevalence was significantly higher in apparently healthy mongrels (77.2 %) and pets (76.9 %) than in defined-breed dogs (including crosses; 58.8 %) and in dogs with an aptitude other than pet (i.e. farm, guard, hunting, shepherd or stray), respectively, whereas in those dogs with at least one clinical sign, the detection of L. infantum DNA was higher in males (53.3 %) and in those dogs not receiving insect repellents (52.8 %).Conclusions: The molecular detection of canine vector-borne disease (CVBD) agents, some of which are zoonotic, reinforces the need to implement efficient prophylactic measures, such as insect repellents and macrocyclic lactones (including compliance to administration), in the geographical areas where these agents are distributed, with the view to prevent infection and disease among mammalian hosts including humans