Family C1 cysteine proteases: Biological diversity or redundancy?

Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzymes, thus inspiring a growing conviction that they may exhibit functional redundancy. This is also supported in part by phenotypes of cathepsin knockout mice and suggests that several proteases can substitute for each other to degrade or process a given substrate. On the other hand, specific functions of one particular protease have also been documented. In addition, differences in cellular distribution and intracellular localization may contribute to defining specific functional roles for some of these proteases

Changes in hemlock looper [Lepidoptera: Geometridae] pupal distribution through a 3-year outbreak cycle

La distribution des chrysalides de l’arpenteuse de la pruche, Lambdina fiscellaria, a été étudiée au cours d’un cycle épidémique d’une durée de trois ans près du Lac Princeton sur l’île d’Anticosti au Québec. Au total, 10 sapins ont été coupés et toutes les chrysalides ont été comptées sur le tronc et les branches (partie non-foliée vs foliée) de la cime inférieure, médiane et supérieure, ainsi que sur le tronc sous la cime. En condition préépidémique, les chrysalides ont principalement été trouvées sur les branches des cimes médianes et supérieures. Durant l’épidémie, la densité des chrysalides n’a pas augmenté dans ces sites de pupaison et les larves se sont surtout transformées en chrysalides sur le tronc, à partir du sol jusque dans la cime médiane, ainsi que sur les branches de la cime inférieure. Peu de chrysalides ont été trouvées sur la partie foliée des branches en période post-épidémique, la plupart étant trouvées sur la partie basale non-foliée qui apparaît comme un endroit préférentiel pour la pupaison de l'arpenteuse de la pruche. De façon à optimiser la détection des augmentations de populations dans les réseaux de surveillance, des pièges à chrysalides devraient être placés à hauteur de poitrine sur le tronc de sapins baumiers.The hemlock looper, Lambdina fiscellaria, pupal distribution was studied through a 3-year outbreak cycle near Lac Princeton on Anticosti Island in Quebec. Over the 3 years, 10 balsam fir trees were cut and all pupae were counted on the stem and branches (non-foliated vs foliated parts) of the lower, middle and upper crowns and on the stem below crown. In pre-outbreak conditions, pupae were mostly found on branches of the middle and upper crowns. During the outbreak, pupal density did not increase on these parts of the trees, since pupae were mostly found on the stem, from the ground to the middle crown, and on branches of the lower crown. Few pupae were found on the foliated portion of branches in post-outbreak conditions but most were found on the basal non-foliated part of branches, which appears to be a preferred location for hemlock looper pupation. In order to optimize detection of population increases in monitoring networks, we suggest using pupal traps at breast height on balsam fir trees

Feasibility of nanofluid-based optical filters

In this article we report recent modeling and design work indicating that mixtures of nanoparticles in liquids can be used as an alternative to conventional optical filters. The major motivation for creating liquid optical filters is that they can be pumped in and out of a system to meet transient needs in an application. To demonstrate the versatility of this new class of filters, we present the design of nanofluids for use as long-pass, short-pass, and bandpass optical filters using a simple Monte Carlo optimization procedure. With relatively simple mixtures, we achieve filters with <15% mean-squared deviation in transmittance from conventional filters. We also discuss the current commercial feasibility of nanofluid-based optical filters by including an estimation of today's off-the-shelf cost of the materials. While the limited availability of quality commercial nanoparticles makes it hard to compete with conventional filters, new synthesis methods and economies of scale could enable nanofluid-based optical filters in the near future. As such, this study lays the groundwork for creating a new class of selective optical filters for a wide range of applications, namely communications, electronics, optical sensors, lighting, photography, medicine, and many more

Towards a Better Understanding of Rural Homelessness: An Examination of Housing Crisis in a Small, Rural Minnesota Community

This report compiles the work done during the Rural Housing Policy course at the University of Minnesota Morris by the students and their instructor, Professor Greg Thorson. the class reviewed the literature on urban and rural homelessness, interviewed local providers of social service programs, developed a survey to be administered at regional homeless shelters, wrote the Institutional Review Board (IRB) proposal to authorize the administration of the survey, administered the survey, and analyzed the results.https://digitalcommons.morris.umn.edu/cst/1009/thumbnail.jp

Rhomboid family member 2 regulates cytoskeletal stress-associated Keratin 16.

Keratin 16 (K16) is a cytoskeletal scaffolding protein highly expressed at pressure-bearing sites of the mammalian footpad. It can be induced in hyperproliferative states such as wound healing, inflammation and cancer. Here we show that the inactive rhomboid protease RHBDF2 (iRHOM2) regulates thickening of the footpad epidermis through its interaction with K16. K16 expression is absent in the thinned footpads of irhom2-/- mice compared with irhom2+/+mice, due to reduced keratinocyte proliferation. Gain-of-function mutations in iRHOM2 underlie Tylosis with oesophageal cancer (TOC), characterized by palmoplantar thickening, upregulate K16 with robust downregulation of its type II keratin binding partner, K6. By orchestrating the remodelling and turnover of K16, and uncoupling it from K6, iRHOM2 regulates the epithelial response to physical stress. These findings contribute to our understanding of the molecular mechanisms underlying hyperproliferation of the palmoplantar epidermis in both physiological and disease states, and how this 'stress' keratin is regulated

Ecohydrologic separation of water between trees and streams in a Mediterranean climate

Water movement in upland humid watersheds from the soil surface to the stream is often described using the concept of translatory flow 1,2 , which assumes that water entering the soil as precipitation displaces the water that was present previously, pushing it deeper into the soil and eventually into the stream 2 . Within this framework, water at any soil depth is well mixed and plants extract the same water that eventually enters the stream. Here we present water-isotope data from various pools throughout a small watershed in the Cascade Mountains, Oregon, USA. Our data imply that a pool of tightly bound water that is retained in the soil and used by trees does not participate in translatory flow, mix with mobile water or enter the stream. Instead, water from initial rainfall events after rainless summers is locked into small pores with low matric potential until transpiration empties these pores during following dry summers. Winter rainfall does not displace this tightly bound water. As transpiration and stormflow are out of phase in the Mediterranean climate of our study site, two separate sets of water bodies with different isotopic characteristics exist in trees and streams. We conclude that complete mixing of water within the soil cannot be assumed for similar hydroclimatic regimes as has been done in the past 3,4 . Links between plant water-use (transpiration) and hydrology have been examined quantitatively since the paired-watershed studies in 1921 (ref. 5). These watershed-scale experiments clearly demonstrated links between vegetation and streamflow. However, the paired-watershed approach can only infer the mechanisms behind these vegetation-streamflow interaction

The impact of loco-regional recurrences on metastatic progression in early-stage breast cancer: a multistate model

To study whether the effects of prognostic factors associated with the occurrence of distant metastases (DM) at primary diagnosis change after the incidence of loco-regional recurrences (LRR) among women treated for invasive stage I or II breast cancer. The study population consisted of 3,601 women, enrolled in EORTC trials 10801, 10854, or 10902 treated for early-stage breast cancer. Data were analysed in a multivariate, multistate model by using multivariate Cox regression models, including a state-dependent covariate. The presence of a LRR in itself is a significant prognostic risk factor (HR: 3.64; 95%-CI: 2.02-6.5) for the occurrence of DM. Main prognostic risk factors for a DM are young age at diagnosis (</=40: HR: 1.79; 95%-CI: 1.28-2.51), larger tumour size (HR: 1.58; 95%-CI: 1.35-1.84) and node positivity (HR: 2.00; 95%-CI: 1.74-2.30). Adjuvant chemotherapy is protective for a DM (HR: 0.66; 95%-CI: 0.55-0.80). After the occurrence of a LRR the latter protective effect has disappeared (P = 0.009). The presence of LRR in itself is a significant risk factor for DM. For patients who are at risk of developing LRR, effective local control should be the main target of therapy

A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity.

Keratin intermediate filaments (KIFs) protect the epidermis against mechanical force, support strong adhesion, help barrier formation, and regulate growth. The mechanisms by which type I and II keratins contribute to these functions remain incompletely understood. Here, we report that mice lacking all type I or type II keratins display severe barrier defects and fragile skin, leading to perinatal mortality with full penetrance. Comparative proteomics of cornified envelopes (CEs) from prenatal KtyI(-/-) and KtyII(-/-)(K8) mice demonstrates that absence of KIF causes dysregulation of many CE constituents, including downregulation of desmoglein 1. Despite persistence of loricrin expression and upregulation of many Nrf2 targets, including CE components Sprr2d and Sprr2h, extensive barrier defects persist, identifying keratins as essential CE scaffolds. Furthermore, we show that KIFs control mitochondrial lipid composition and activity in a cell-intrinsic manner. Therefore, our study explains the complexity of keratinopathies accompanied by barrier disorders by linking keratin scaffolds to mitochondria, adhesion, and CE formation