Culture of human cell lines by a pathogen-inactivated human platelet lysate

Alternatives to the use of fetal bovine serum (FBS) have been investigated to ensure xeno-free growth condition. In this study we evaluated the efficacy of human platelet lysate (PL) as a substitute of FBS for the in vitro culture of some human cell lines. PL was obtained by pools of pathogen inactivated human donor platelet (PLT) concentrates. Human leukemia cell lines (KG-1, K562, JURKAT, HL-60) and epithelial tumor cell lines (HeLa and MCF-7) were cultured with either FBS or PL. Changes in cell proliferation, viability, morphology, surface markers and cell cycle were evaluated for each cell line. Functional characteristics were analysed by drug sensitivity test and cytotoxicity assay. Our results demonstrated that PL can support growth and expansion of all cell lines, although the cells cultured in presence of PL experienced a less massive proliferation compared to those grown with FBS. We found a comparable percentage of viable specific marker-expressing cells in both conditions, confirming lineage fidelity in all cultures. Functionality assays showed that cells in both FBS- and PL-supported cultures maintained their normal responsiveness to adriamycin and NK cell-mediated lysis. Our findings indicate that PL is a feasible serum substitute for supporting growth and propagation of haematopoietic and epithelial cell lines with many advantages from a perspective of process standardization, ethicality and product safety

Numerical and experimental comparison among a new hybrid FT-music technique and existing algorithms for through-the-wall radar imaging

A fast low-cost through-the-wall radar imaging (TWRI) system, based on a vector network analyzer (VNA), a couple of switches and an array of Vivaldi antennas, has been designed, realized, and tested. To solve the TWRI inversion problem, an original theoretical modeling for a class of TWRI techniques whose basic functions are the cross-range Fourier transform (FT) of the scattered field and its covariance operator has been proposed. Using these functions, four conventional algorithms, namely the delay and sum (DAS), the FT, the multiple signal classification (MUSIC), the hybrid DAS-MUSIC and a new algorithm, the hybrid FT-MUSIC, have been derived. All these techniques have been implemented and their accuracy and field of view have been tested on canonical scatterers. Then, the algorithms have been applied to measured data collected in different scenarios constituted by a metallic bar or a human subject in the absence and in the presence of a wall between the antenna and the considered targets. Using the proposed TWRI system, it has been possible to detect a subject located up to 5-m away from the radar antenna array through a tuff wall. The proposed FT-MUSIC algorithm has evidenced performances similar to those of the DAS-MUSIC but with significantly lower execution times. Finally, FT-MUSIC performances in terms of field of view and immunity to disturbances are better compared to those of the MUSIC algorithm

Energy/Environment Models: Relationship to Planning in Wisconsin, GDR, Rhone Alps

This report is a description and cross-regional comparison of the institutional structures and modeling methodologies of the three regions participating in the IIASA Research Program on Management of Regional Energy/Environment Systems. Descriptions are presented for the state of Wisconsin (USA), the German Democratic Republic, and the Rhone-Alpes Region (France), by specialists and policy makers from the respective regions. These descriptions demonstrate quite vividly the relationships between the institutional structure of a region and its use of models and planning tools

More than maternal sensitivity shapes attachment : infant coping and temperament

The aim of this longitudinal studywas to investigate the effect of a set of factors from multiple levels of influence: infant temperament, infant regulatory behavior, and maternal sensitivity on infant’s attachment. Our sample consisted of 48 infants born prematurely and their mothers. At 1 and 3 months of age, mothers described their infants’behavior using the Escala de Temperamento do Beb´e. At 3 months of age, infants’ capacity to regulate stress was evaluated during Tronick’s Face-to-Face Still-Face (FFSF) paradigm. At 9 months of age, mothers’ sensitivity was evaluated during free play using the CARE-Index. At 12 months of age, infants’ attachment security was assessed during Ainsworth’s Strange Situation. A total of 16 infants were classified as securely attached, 17 as insecure-avoidant, and 15 as insecure-resistant. Mothers of securely attached infantswere more likely than mothers of insecure infants to describe their infants as less difficult and to be more sensitive to their infants in free play. In turn, secure infants exhibited more positive responses during the Still-Face. Infants classified as insecureavoidant were more likely to self-comfort during the Still-Face and had mothers who were more controlling during free play. Insecure-resistant exhibited higher levels of negative arousal during the Still-Face and had mothers who were more unresponsive in free play. These findings show that attachment quality is influenced bymultiple factors, including infant temperament, coping behavior, and maternal sensitivity

The impact of protocol assignment for older adolescents with Hodgkin lymphoma

Background and Purpose: Hodgkin lymphoma (HL) treatment has evolved to reduce or avoid radiotherapy (RT) dose and volume and minimize the potential for late effects. Some older adolescents are treated on adult protocols. The purpose of this study is to examine the protocol assignment of older adolescents and its impact on radiation dose to relevant thoracic structures. Materials and Methods: Cooperative group data were reviewed and 12 adolescents were randomly selected from a pediatric HL protocol. Treatment plans were generated per one pediatric and two adult protocols. Dose volume histograms for heart, lung, and breast allowed comparison of radiation dose to these sites across these three protocols. Results: A total of 15.2% of adolescents were treated on adult HL protocols and received significantly higher radiation dosage to heart and lung compared to pediatric HL protocols. Adolescents treated on either pediatric or adult protocols received similar RT dose to breast. Conclusion: Older adolescents treated on adult HL protocols received higher RT dose to thoracic structures except breast. Level of nodal involvement may impact overall RT dose to breast. The impact of varying field design and RT dose on survival, local, and late effects needs further study for this vulnerable age group

Implementing QVT-R bidirectional model transformations using alloy

QVT Relations (QVT-R) is the standard language proposed by the OMG to specify bidirectional model transformations. Unfortunately, in part due to ambiguities and omissions in the original semantics, acceptance and development of effective tool support has been slow. Recently, the checking semantics of QVT-R has been clarified and formalized. In this paper we propose a QVT-R tool that complies to such semantics. Unlike any other existing tool, it also supports meta-models enriched with OCL constraints (thus avoiding returning ill-formed models), and proposes an alternative enforcement semantics that works according to the simple and predictable “principle of least change”. The implementation is based on an embedding of both QVT-R transformations and UML class diagrams (annotated with OCL) in Alloy, a lightweight formal specification language with support for automatic model finding via SAT solving.Fundação para a Ciência e a Tecnologi

Prioritization of high-cost new drugs for HCV: making sustainability ethical

Hepatitis C virus (HCV) infection is a major health problem worldwide. Chronic HCV infection may in the long run cause cirrhosis, hepatic decompensation and hepatocellular carcinoma, with an ultimate disease burden of at least 350,000 deaths per year worldwide. The new generation of highly effective direct acting antivirals (DAA) to treat HCV infection brings major promises to infected patients in terms of exceedingly high rates of sustained virological response (SVR) but also of tolerability, allowing even the sickest patients to be treated. Even in the face of the excellent safety and efficacy and wide theoretical applicability of these regimens, their introduction is currently facing cost and access issues denying their use to many patients in need. Health systems in all countries are facing a huge problem of distributive justice, since while they should guarantee individual rights, among which the right to health in its broader sense, therefore not limited to healing, but extended to quality of life, they must also grant equal access to the healthcare resources and keep the distribution system sustainable. In the face of a disease with a relatively unpredictable course, where many but not of all chronically infected will eventually die of liver disease, selective allocation of this costly resource is debatable. In most countries the favorite solution has been a stratification of patients for prioritization of treatment, which means allowing Interferon-free DAA treatment only in patients with advanced fibrosis or cirrhosis, while keeping on hold persons with lesser stages of liver disease. In this report, we will perform an ethical assessment addressing the issues linked to access to new therapies, prioritization and eligibility criteria, analyzing the meaning of the term “distributive justice” and the different approaches that can guide us (individualistic libertarianism, social utilitarianism and egalitarianism) on this specific matter. Even if over time the price of new DAA will be reduced through competition and eventual patent expiration, the phenomenon of high drug costs will go on in the next decades and we need adequate tools to face the problems of distributive justice that come with it

Metodología para evaluar el desvanecimiento del refinador de grano en aleaciones bifásicas

La vida de las partículas refinantes debe ser valorada por algún tipo de ensayo; si bien es fácil en aleaciones que solidifican como soluciones sólidas monofásicas o con pequeñas segundas fases, es realmente difícil en latones bifásicos debido a que la mezcla de fases enmascara el grano refinado. Por lo tanto, el objetivo de este trabajo experimental es desarrollar un método para evaluar el tiempo de vida de un refinador de grano en un latón amarillo al plomo.Sección: Mecánica.Facultad de Ingenierí

Best practice considerations on the assessment of robotic assisted surgical systems:results from an international consensus expert panel

Background Health technology assessments (HTAs) of robotic assisted surgery (RAS) face several challenges in assessing the value of robotic surgical platforms. As a result of using different assessment methods, previous HTAs have reached different conclusions when evaluating RAS. While the number of available systems and surgical procedures is rapidly growing, existing frameworks for assessing MedTech provide a starting point, but specific considerations are needed for HTAs of RAS to ensure consistent results. This work aimed to discuss different approaches and produce guidance on evaluating RAS. Methods A consensus conference research methodology was adopted. A panel of 14 experts was assembled with international experience and representing relevant stakeholders: clinicians, health economists, HTA practitioners, policy makers, and industry. A review of previous HTAs was performed and seven key themes were extracted from the literature for consideration. Over five meetings, the panel discussed the key themes and formulated consensus statements. Results A total of ninety-eight previous HTAs were identified from twenty-five total countries. The seven key themes were evidence inclusion and exclusion, patient- and clinician-reported outcomes, the learning curve, allocation of costs, appropriate time horizons, economic analysis methods, and robotic ecosystem/wider benefits. Conclusions Robotic surgical platforms are tools, not therapies. Their value varies according to context and should be considered across therapeutic areas and stakeholders. The principles set out in this paper should help HTA bodies at all levels to evaluate RAS. This work may serve as a case study for rapidly developing areas in MedTech that require particular consideration for HTAs.</p

Solving Constraints in Model Transformations

Constraint programming holds many promises for model driven software development (MDSD). Up to now, constraints have only started to appear in MDSD modeling languages, but have not been properly reflected in model transformation. This paper introduces constraint programming in model transformation, shows how constraint programming integrates with QVT Relations - as a pathway to wide spread use of our approach - and describes the corresponding model transformation engine. In particular, the paper will illustrate the use of constraint programming for the specification of attribute values in target models, and provide a qualitative evaluation of the benefit drawn from constraints integrated with QVT Relations