Science and technology innovation and practice of major national science and technology special project of coalbed methane in coal mining area

The scientific and technological innovation system formed by the national major oil and gas projects has the characteristics of “new nationwide innovation system”, and the system of scientific and technological projects has the characteristics of “whole process, multi-stage and full life cycle”. During the 11th Five-Year Plan period and 13rd Five-Year Plan period, major national oil and gas projects have continuously supported scientific and technological breakthroughs and technological innovations in basic theories, key technologies and complete sets of equipment for the development and utilization of coalbed methane. Members of the national major projects of oil and gas projects and project implementation unit, paying great efforts, aim to taking the national major projects of oil and gas as an great opportunity, to enhance the level of coal mine area of coalbed methane of science and technology innovation and form the leading industry of coal mining area of coal bed methane research and development of science and technology innovation system, to fully support the realization of key scientific research goals of major national oil and gas projects. Coal mining area coal bed methane grograms have formed a series of major landmark achievements, built a series of industry innovation platform, comprehensively stired the technological progress and industrial development of coal-bed methane industry in coal mining areas of China, and effectively promoted the construction of the coal-bed methane industry in China. Combined with the organization and implementation of the project (subject)/demonstration project and the characteristics of the coal industry, we have systematically summarize the exploration and practice of CBM technological innovation in national major special coal mine area and formed a national major special project of science and technology, which is “guided by national investment, dominated by enterprise investment, highly integrated with industry, university, research and application, and continuously leading the technological progress of the industry”. In establishing and perfecting the organization and management institutions, continue to strengthen the special management specification, continue to play a role of cohesive ties, coordination to promote the scientific research management innovation, promote the development and use fusion to form coal mining area of coalbed methane from the concrete experience of science and technology innovation practice

Artemisinin Targets Transcription Factor PDR1 and Impairs Candida glabrata Mitochondrial Function

A limited number of antifungal drugs, the side-effect of clinical drugs and the emergence of resistance create an urgent need for new antifungal treatment agents. High-throughput drug screening and in-depth drug action mechanism analyzation are needed to address this problem. In this study, we identified that artemisinin and its derivatives possessed antifungal activity through a high-throughput screening of the FDA-approved drug library. Subsequently, drug-resistant strains construction, a molecular dynamics simulation and a transcription level analysis were used to investigate artemisinin’s action mechanism in Candida glabrata. Transcription factor pleiotropic drug resistance 1 (PDR1) was an important determinant of artemisinin’s sensitivity by regulating the drug efflux pump and ergosterol biosynthesis pathway, leading to mitochondrial dysfunction. This dysfunction was shown by a depolarization of the mitochondrial membrane potential, an enhancement of the mitochondrial membrane viscosity and an upregulation of the intracellular ROS level in fungi. The discovery shed new light on the development of antifungal agents and understanding artemisinin’s action mechanism