Analysis of some factors influencing the epidemiology of Bluetongue Virus in ruminants. A hypothesis of control strategy through decrease of Culicoides and their associated damage in farm

The main object of this research has been to apply the scientific assumption that the alkalization is able to make inhospitable the larval reproductive sites in the identified area (Sant’Antioco). Moreover several studies about the reproductive behaviour of BT vectors has been conducted. First every data about BT history in Italy and especially in Sardinia had been gathered, then a epidemiologic retrospective view of the events in the whole island has been issued to study the disease prevalence and how environmental and geo-pedological factors have been able to change this prevalence. The research has been conducted in the pilot farm into the selected area of Sant’Antioco where the first hypothesis has been tested and every information about larval development has been collected. During the whole experimental period have been collected all the data about captures in the light traps and captures in the traps where midges were emerging from the larval case in both areas, alkalized and not. Furthermore have been performed the epidemiologic model taking into account the local epidemiological data and the several variables that were involved in the damage registered in every farm of the island of Sant’Antioco. Experimental data first of all have been emphasize the possibility of cut down the larval reproduction extremely in the alkalized sites then have allowed to study the BT vectors density closely and its relation with the disease prevalence in the heads presents in the near farms. The results revealed the different distribution of the disease prevalence in the several farms, referable to minute variation of the farm location. In the end it seems essential to improve the farm management through the widespread action of awakening to farmers

Application of anammox within an integrated approach to sustainable food waste management and valorization

In this study, the anammox process was applied for the first time to the treatment of ammonium-rich liquid residues produced by the two-stage anaerobic digestion of food waste (2sAD-FW); such residues may represent a significant environmental issue if not properly managed. A granular anammox reactor was fed with a progressively increasing share of partially nitritated 2sAD-FW wastewater. An alternative operating strategy based on partial by-pass of the partial nitritation unit was tested, in order to regulate the influent NO2/NH4 molar ratio without chemical addition. High nitrogen removal efficiency (89 ± 1%) and negligible nitrite discharge rates were achieved, together with high nitrogen removal rate / nitrogen loading rate (NRR/NLR, 97 ± 1%) and stable specific anammox activity (0.42 ± 0.03 gN2-N/gVSS·d). The observed NH4-removed/NO2-removed/NO3-produced molar ratio was in agreement with anammox stoichiometry, as confirmed by the low contribution (< 5%) of denitrification to nitrogen removal. Moreover, the possibility of using digital color characterization of granular biomass as a novel, simple tool for the monitoring of anammox biomass enrichment and process performance was investigated under dynamic conditions, using real wastewater: changes in granule color correlated well with the increasing share of 2sAD-FW wastewater in the influent (R2 = 83%), as well as with the decrease of anammox biomass abundance in the reactor (R2 = 68%). The results suggest that anammox may be successfully integrated into a 2sAD-FW system, thus enhancing its environmental sustainability

Sustainable recovery of secondary and critical raw materials from classified mining residues using mycorrhizal-assisted phytoextraction

In this work, mycorrhizal-assisted phytoextraction (MAP, Helianthus annuus–arbuscular mycorrhizal fungus Rhizophagus intraradices–Zn-volcanic ashes) was applied for the recovery of secondary and critical raw materials (SRMs and CRMs, respectively) from Joda West (Odisha, India) mine residues, within a novel multidisciplinary management strategy. Mine residues were preliminarily characterized by using advanced analytical techniques, and subsequently mapped, classified and selected using multispectral satellite Sentinel-2A images and cluster analysis. Selected mine residues were treated by MAP at laboratory scale, and the fate of several SRMs (e.g., Zn, Cr, As, Ni, Cu, Ca, Al, K, S, Rb, Fe, Mn) and CRMs (such as Ga, Ti, P, Ba and Sr) was investigated. Bioconcentration factors in shoots (BCS) and roots (BCR) and translocation factors (TF) were: 5.34(P) > BCS > 0.00(Al); 15.0(S) > BCR > 0.038(Ba); 9.28(Rb) > TF > 0.02(Ti). Results were used to predict MAP performance at larger scale, simulating a Vegetable Depuration Module (VDM) containing mine residues (1 m3). Estimated bio-extracting potential (BP) was in the range 2417 g/m3 (K) > BP> 0.14 g/m3 (As), suggesting the eventual subsequent recovery of SRMs and CRMs by hydrometallurgical techniques, with final purification by selective electrodeposition, as a viable and cost-effective option. The results are promising for MAP application at larger scale, within a circular economy-based approach.Fil: Scotti, Adalgisa. Comisión Nacional de Energía Atómica; ArgentinaFil: Stefano, Milia. Consiglio Nazionale delle Ricerche; ItaliaFil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Giovanna, Cappai. Consiglio Nazionale delle Ricerche; ItaliaFil: Guglietta, Daniela. Consiglio Nazionale delle Ricerche; ItaliaFil: Francesca, Trapasso. Consiglio Nazionale delle Ricerche; ItaliaFil: Emanuela, Tempesta. Consiglio Nazionale delle Ricerche; ItaliaFil: Daniele, Passeri. Consiglio Nazionale delle Ricerche; ItaliaFil: Godeas, Alicia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Gomez, Martin. Comisión Nacional de Energía Atómica; ArgentinaFil: Stefano, Ubaldini. Consiglio Nazionale delle Ricerche; Itali

Predictors of Bluetongue development in Sardinia (Italy) identification, using multilevel logistic mixed model

Objectives: The Bluetongue Virus is one of the most studied ruminant diseases, affecting particularly sheep and goats. This study aims to identify, for the first time, the specific risk factors influencing the disease development in Sardinia, using multilevel logistic regression model, in order to give a contribution to the sanitary programs and favour the early detection.   Methods: The data of the present retrospective study, collected from informatics systems of Istituto Zooprofilattico della Sardegna, are referred to all 15,780 Sardinian sheep farms observed for 3 years (2012-2014). The outcome of interest was dichotomous and defined the development of Bluetongue outbreak, after serological test or clinical signs. The effect of several region-specific prognostic factors on disease spread was investigated. Results: The final model indicated that Bluetongue development was significantly associated with an increase in number of animals (P < 0.0001), number of cattle around farm (P  < 0.0001), water surface area (P =0.002), and amount of rainfall in the previous days (P < 0.0001). Furthermore, the altitude over 450 MASL (P < 0.0001), the vaccination prophylaxis (P < 0.0001) and the previous outbreak event (P < 0.0001) had a protective effect against the outcome. Conclusion: The results of this study indicated that number of animals and the amount of rainfall were the most important risk factors that affected the Bluetongue development, while the vaccination prophylaxis was found to be an effective measure in decelerating the disease spread.&nbsp

Scale-up of Mycorrhizal-Assisted Phytoremediation system from Technology Readiness Level 6 (Relevant Environment) to 7 (Operational Environment): Cost-benefits within a Circular Economy Context

This chapter analyzes the costs-benefits of a particular phytomining methodology named mycorrhizal-assisted phytoremediation (MAP). This MAP system is responsible for phytostabilization and/or phytoextraction of secondary and critical raw materials from contaminated soil or mining wastes. To this aim, we evaluated the application of MAP in a modified constructed wetland, the vegetable depuration module (VDM), which permits the calibration of physical-chemical-biological variables in a contaminated substrate, as well as the partition of chemical elements within the liquid phase due to leaching and solid phases (biomass and soil). This successful methodology allows to scale-up from a Technology Readiness Level (TRL) 6 (demonstration in a relevant environment) toward TRL 7 (demonstration in an operational environment), which implies the transfer to the territory

Diffuse C-Cells Hyperplasia Is the Source of False Positive Calcitonin Measurement in FNA Washout Fluids of Thyroid Nodules: A Rational Clinical Approach to Avoiding Unnecessary Surgery

Purpose: The FNA-CT is useful for the diagnosis of MTC. The aim of this study was to evaluate the performance of FNA-CT in TNs coexisting with CCH. Methods: This study retrospectively reviewed the records of 11 patients with TNs submitted to thyroidectomy on the basis of elevated basal and/or stimulated serum CT values, which at histology were not confirmed to be MTC. The results obtained in this group were compared with those of a previously reported group of histologically proven MTC patients submitted to an identical presurgical evaluation. All patients, negative for known mutations in the RET proto-oncogene, were preoperatively submitted to neck ultrasound, FNA-cytology, and FNA-CT. Results: Approximately 6 of 11 patients showed increased (>36 ng/mL, as established in previous studies not involving patients with CCH) FNA-CT. All these patients showed diffuse CCH at histology in the thyroid lobe submitted to FNA; 5 of them were benign at histology, while only one was malignant (papillary thyroid carcinoma, PTC). The remaining 5 of 11 patients had low FNA-CT (<36 ng/mL), and all of them showed only focal CCH in the lobe submitted to FNA; three of them were malignant (2 PTC, 1 follicular carcinoma), while two were benign. Conclusions: Employing the currently proposed cut-off values, false-positive FNA-CT results may be observed in benign/malignant TNs with coexisting diffuse CCH. FNA-CT must therefore be cautiously used in the diagnostic approach for patients with TNs and a slightly increased basal or stimulated serum CT concentration in order to avoid unnecessary surgery

Functional characterization of a CDKN1B mutation in a Sardinian kindred with multiple endocrine neoplasia type 4 (MEN4)

Inactivating germline mutations of the CDKN1B gene, encoding for the nuclear cyclin-dependent kinase inhibitor p27kip1 protein, have been reported in patients with multiple endocrine neoplasia type 4 (MEN4), a MEN1-like phenotype without MEN1 mutations. The aim of this study was to in vitro characterize the germline CDKN1B mutation c.374_375delCT (S125X) we detected in a patient with MEN4. The proband was affected by multiglandular primary hyperparathyroidism and gastro-entero-pancreatic tumors. We carried out subcellular localization experiments transfecting into eukaryotic HeLa and GH3 cell lines plasmid vectors expressing the CDKN1B wild type (wt) or mutant cDNA. Western blot studies showed that fusion proteins were expressed at equal levels. The mutated protein was shorter compared to the wt protein and lacked the highly conserved C-terminal domain, which includes the bipartite nuclear localization signal at amino acids 152/153 and 166/168. In HeLa and GH3 cells wt p27 localized in the nucleus whereas the p27_S125X protein was retained in the cytoplasm predicting the loss of tumor suppressive function. The proband's tumoral parathyroid tissue did not show allelic loss, since wt and mutant alleles were both present by sequencing the somatic DNA. Immunohistochemistry showed a complete loss of nuclear p27 expression in the parathyroid adenoma removed by the patient at the second surgery. In conclusion, our study confirms the pathogenic role of the c.374_375delCT CDKN1B germline mutation in a patient with MEN4

Functional characterization of a CDKN1B mutation in a Sardinian kindred with multiple endocrine neoplasia type 4 (MEN4)

Inactivating germline mutations of the CDKN1B gene, encoding for the nuclear cyclin-dependent kinase inhibitor p27kip1 protein, have been reported in patients with multiple endocrine neoplasia type 4 (MEN4), a MEN1-like phenotype without MEN1 mutations. The aim of this study was to in vitro characterize the germline CDKN1B mutation c.374_375delCT (S125X) we detected in a patient with MEN4. The proband was affected by multiglandular primary hyperparathyroidism and gastro-entero-pancreatic tumors. We carried out subcellular localization experiments transfecting into eukaryotic HeLa and GH3 cell lines plasmid vectors expressing the CDKN1B wild type (wt) or mutant cDNA. Western blot studies showed that fusion proteins were expressed at equal levels. The mutated protein was shorter compared to the wt protein and lacked the highly conserved C-terminal domain, which includes the bipartite nuclear localization signal at amino acids 152/153 and 166/168. In HeLa and GH3 cells wt p27 localized in the nucleus whereas the p27_S125X protein was retained in the cytoplasm predicting the loss of tumor suppressive function. The proband's tumoral parathyroid tissue did not show allelic loss, since wt and mutant alleles were both present by sequencing the somatic DNA. Immunohistochemistry showed a complete loss of nuclear p27 expression in the parathyroid adenoma removed by the patient at the second surgery. In conclusion, our study confirms the pathogenic role of the c.374_375delCT CDKN1B germline mutation in a patient with MEN4

The rhythm of the night: patterns of~activity of the European wildcat in the Italian peninsula

The European wildcat is a threatened carnivore, whose ecology is still scarcely studied, especially in Mediterranean areas. In this study, we estimated activity rhythm patterns of this felid, by means of camera-trapping at three spatial scales: (i) whole country (Italy); (ii) biogeographical areas; (iii) latitudinal zones. The activity rhythms patterns were also calculated according to temporal scales: (1) warm semester; (2) cold semester and (3) seasonal scales. Lastly, we also tested whether the effect of moon phases affected the wildcat activity. We conducted the analysis on a total of 975 independent events collected in 2009-2021, from 285 locations, in 65,800 camera days. We showed that the wildcat in Italy exhibits a > 70% nocturnal behaviour, with 20% of diurnal activity, at all spatial scales, and throughout the whole year, with peaks at 10.00 p.m. and 04.00 a.m. We observed a high overlap of wildcat activity rhythms between different biogeographical and latitudinal zones. The wildcat was mainly active on the darkest nights, reducing its activity in bright moonlight nights. Diurnal activity was greater in the warm months and decreased with the distance from shrubs and woodlands, most likely according to activity rhythms of its main prey, water presence in summer, the care of offspring and the availability of shelter sites. Conversely, the distance to paved roads seems to have no significant effects on diurnal activity, suggesting that, in presence of natural shelters, the wildcat probably may tolerate these infrastructures. We suggested limited plasticity in activity rhythm patterns of the wildcat, emphasizing the importance of dark hours for this species

Toward a Multidisciplinary Strategy for the Classification and Reuse of Iron and Manganese Mining Wastes

The aim of this paper is to evaluate an integrated multidisciplinary strategy for the characterization of mining waste, their possible recycling and reuse. The use of Fe-Mn rich wastes in arsenic removal and phosphorus recovery from water and the phytoextraction potential of metals and their possible recovery from biomass are evaluated