Deconstructing sugarcane bagasse lignocellulose by acid-based deep eutectic solvents to enhance enzymatic digestibility

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGBiorefinery with deep eutectic solvent (DES) is an emerging processing technology to overcome the shortcomings of conventional biomass pretreatments. This work evaluates the biorefinery of sugarcane bagasse (SCB) with DES formulated with choline chloride as hydrogen bond acceptor and three hydrogen bond donors: lactic acid, citric acid, and acetic acid. Acetic acid showed unique ionic properties responsible for the selective removal of lignin and the deconstruction of cellulose to improve the digestibility of up to 97.61 % of glucan and 63.95 % of xylan during enzymatic hydrolysis. In addition, the structural characteristics of the polysaccharide-rich material (PRM) were analyzed by X-rays, ATR-FTIR, SEM, and enzymatic hydrolysis, and compared with the original material sample, for a comprehensive understanding of biomass deconstruction using different hydrogen bond donors (HBD) as DES pretreatment.Ministerio de Ciencia e Innovación | Ref. PID2020-115879RB-I00São Paulo​ Research Foundation | Ref. 2018/25511-1São Paulo​ Research Foundation | Ref. 2021/15138-4National Council for Scientific and Technological Development—CNPq | Ref. 312923/2020-1National Council for Scientific and Technological Development—CNPq | Ref. 408783/2021-4Xunta de Galicia | Ref. GPC-ED431B 2021/2

Effect of Maltodextrin Reduction and Native Agave Fructans Addition on the Rheological Behavior of Spray-Dried Juices

Agave fructans have thermal protective and encapsulating properties as well as technological functions as stabilizers. The effect of the combination of maltodextrin 10% [w/v] and native agave fructans in concentrations of 0, 2, and 4% [w/v] on the rheological properties and microstructure, of spray-dried chayote, carrot, mango and pineapple powders was evaluated. The flow behavior was analyzed in a simple shear flow and low-cutting speed in the range of 5–200 s−1. The experimental data of fresh or reconstituted juices were fitted to different flow models such as Newtonian, Bingham, and Ostwald-de-Waele. The flow behavior of all juices can be described by the Bingham model with low plastic viscosities; the addition of fructans and the step of spray drying had no significant influence on the plastic viscosity of juices as compared to fresh juices

Preparation and Characterization of Electrospun Polysaccharide FucoPol-Based Nanofiber Systems

The electrospinnability of FucoPol, a bacterial exopolysaccharide, is presented for the first time, evaluated alone and in combination with other polymers, such as polyethylene oxide (PEO) and pullulan. The obtained fibers were characterized in terms of their morphological, structural and thermal properties. Pure FucoPol fibers could not be obtained due to FucoPol’s low water solubility and a lack of molecular entanglements. Nanofibers were obtained via blending with PEO and pullulan. FucoPol:PEO (1:3 w/w) showed fibers with well-defined cylindrical structure, since the higher molecular weight of PEO helps the continuity of the erupted jet towards the collector, forming stable fibers. WAXS, DSC and TGA showed that FucoPol is an amorphous biopolymer, stable until 220◦C, whereas FucoPol-PEO fibers were stable until 140◦C, and FucoPol:pullulan fibers were stable until 130◦C. Interestingly, blended components influenced one another in intermolecular order, since new peaks associated to intermolecular hierarchical assemblies were seen by WAXS. These results make FucoPol-based systems viable candidates for production of nanofibers for packaging, agriculture, biomedicine, pharmacy and cosmetic applications

Structural Modification of Jackfruit Leaf Protein Concentrate by Enzymatic Hydrolysis and Their Effect on the Emulsifier Properties

Jackfruit leaf protein concentrate (LPC) was hydrolyzed by pepsin (H–Pep) and pancreatin (H–Pan) at different hydrolysis times (30–240 min). The effect of the enzyme type and hydrolysis time of the LPC on the amino acid composition, structure, and thermal properties and its relationship with the formation of O/W emulsions were investigated. The highest release of amino acids (AA) occurred at 240 min for both enzymes. H–Pan showed the greatest content of essential and hydrophobic amino acids. Low β-sheet fractions and high β-turn contents had a greater influence on the emulsifier properties. In H–Pep, the β-sheet fraction increased, while in H–Pan it decreased as a function of hydrolysis time. The temperatures of glass transition and decomposition were highest in H–Pep due to the high content of β-sheets. The stabilized emulsions with H–Pan (180 min of hydrolysis) showed homogeneous distributions and smaller particle sizes. The changes in the secondary structure and AA composition of the protein hydrolysates by the effect of enzyme type and hydrolysis time influenced the emulsifying properties. However, further research is needed to explore the use of H–Pan as an alternative to conventional emulsifiers or ingredients in functional foods

Application of essential oils and polyphenols as natural antimicrobial agents in postharvest treatments: Advances and challenges

The use of natural antimicrobial agents is an attractive ecological alternative to the synthetic fungicides applied to control pathogens during postharvest. In order to improve industrial production systems, postharvest research has evolved toward integration with science and technology aspects. Thus, the present review aims to draw attention to the achieved advances and challenges must be overcome, to promote application of essential oils and polyphenols as antimicrobial agents, against phytopathogens and foodborne microorganisms during postharvest. Besides that, it attempts to highlight the use of coating and encapsulation techniques as emerging methods that improve their effectiveness. The integral knowledge about the vegetable systems, molecular mechanisms of pathogens and mechanisms of these substances would ensure more efficient in vitro and in vivo experiences. Finally, the cost-benefit, toxicity, and ecotoxicity evaluation will be guaranteed the successful implementation and commercialization of these technologies, as a sustainable alternative to minimize production losses of vegetable commodities

Identification of Saccharomyces cerevisiae strains for alcoholic fermentation by discriminant factorial analysis on electronic nose signals

Axe 2 Structuration sous contraintes des agropolymères et Réactivité des poudres . Available from Internet: http://www.ejbiotechnology.cl/content/vol13/issue4/full/11/index.html. ISSN 0717-3458. Contact: [email protected] audienceAn electronic nose (E-nose) coupled to gas chromatography was tested to monitor alcoholic fermentation by Saccharomyces cerevisiae ICV-K1 and Saccharomyces cerevisiae T306, two strains well-known for their use in oenology. The biomass and ethanol concentrations and conductance changes were measured during cultivations and allowed to observe the standard growth phases for both yeast strains. The two strains were characterized by a very similar tendency in biomass or ethanol production during the fermentation. E-nose was able to establish a kinetic of the production of aroma compounds production and which was then easy to associate with the fermentation phases. Principal Component Analysis (PCA) showed that the data collected by E-nose during the fermentation mainly contained cultivation course information. Discriminant factorial analysis (DFA) was able to clearly identify differences between the two strains using the four main principal components of PCA as input data. Nevertheless, the electronic nose responses being mainly influenced by cultivation course, a specific data treatment limiting the time influence on data was carried out and permitted to achieve an overall performance of 83.5

Development of antifungal electrospun nanofiber mats containing Meyerozyma caribbica

To mitigate vegetable and fruit loss caused by post-harvest fungal diseases, polymeric antifungal coatings encapsulating biocontrol yeasts offer a sustainable alternative. Nanofibers derived from pullulan, cashew gum, FucoPol and poly (ethylene oxide) (PEO), were electrospun and loaded with Meyerozyma caribbica (GenBank ID: JQ398674). The morphological, thermal and chemical properties of cashew gum (CG:PEO), FucoPol (FP:PEO), and pullulan nanofibers were characterized. The viability of M. caribbica within the fibers and their in vitro antifungal activity against six phytopathogens were assessed. Morphological analysis exhibited the presence of nanofibers encapsulating M. caribbica. ATR-FTIR spectroscopy identified the absence of interactions between the yeast and polymers. Fibers containing M. caribbica exhibited in vitro fungistatic effects on spore germination. Pullulan nanofibers showed the highest M. caribbica viability and the highest percentage of growth inhibition against the evaluated fungi. These promising nanofibers encapsulating biocontrol yeasts could be used as edible coatings or agricultural aids, which offer an alternative for post-harvest treatment to control fungal diseases, reducing global food loss.This work was supported by the CYTED thematic network (code 319RT0576), the Agencia Valenciana de Innovación (AVI), projects INNEST/2022/25 and AGROALNEXT/2022/058, the grant PID2021-128749OB-C31 funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe.Peer reviewe

Effect of maltodextrin reduction and native agave fructans addition on the physicochemical properties of spray-dried mango and pineapple juices

International audienceThe effects of the partial replacement of maltodextrin by native agave fructans on the characteristics of spray-dried pineapple and mango powder were evaluated in this study. An experimental 33 design, three concentrations of maltodextrin (5, 7, and 10%), three concentrations of native agave fructans (0, 2, and 4%), and three feed temperatures (110, 115, and 120  ℃) were used. The results using the treatment in which only maltodextrin was used as a reference indicated that an increment in the inlet temperature decreases the moisture content, aw, and solubility. Likewise, an increase (more than 2%) in fructans concentration generates products with increased aw, moisture, hygroscopicity, wettability, and greater solubility. Additionally, no modification of storage stability was observed. Mango and pineapple powder color were affected mainly by the inlet temperature, causing an increase in luminosity (L*) and a decrease in parameter ( a*). A scanning electron microscopy showed spherical powder particles with certain contractions; powder stability in treatments with native agave fructans was not modified in the treatment at 2%. Finally, the addition of 2% agave fructans as carrier material was able to reduce the maltodextrin concentration of the spray drying process

Antimicrobial soy protein based coatings: Application to Persian lime ( Citrus latifolia Tanaka) for protection and preservation

correction DOI: 10.1016/j.postharvbio.2017.12.009 (WOS:000418891000018)Preventive antifungal activity of postharvest treatments with SPI (soy protein isolate) −coatings forming solutions against blue mould decay were evaluated on Persian lime (Citrus latifolia Tanaka) artificially inoculated in rind wounds with Penicillium italicum. Stimulatory effects were observed with the use of citral, however with the use of limonene an inhibitory effect was obtained. After 13 d of storage, significant preventive activity against blue mould was observed with a 20% of disease incidence applying SPI-coating forming solutions with limonene added. SEM micrographs showed that limonene could act as an inhibitor of germ tube elongation, delaying the process of infection. Quality parameters like water losses, change in colour and aroma compounds release were assessed on fruit coated. Depending to storage condition, SPI-coating forming solutions were effective to reduce water losses, maintain colour and controlling the liberation of active agent