The value of laparoscopic classifications in decision on definitive surgery in patients with nonpalpable testes: our experience and review of the literature

Background/purpose The aim of the study was to present our clinical experience with the laparoscopic approach in patients with nonpalpable testes (NPTs) and review the literature on laparoscopic classifications.Materials and methods Between May 2010 and August 2012, 30 boys with NPT (mean age 3.9 years) underwent laparoscopy as a part of diagnosis and treatment in our clinic. The laparoscopic findings were classified into four types according to Castilho. The patients were managed according to the presence or position of the testes and testicular vessels.Results Six patients were excluded from the study. Twenty-six testicular units (19 left, three right, and four bilateral units) in 24 patients were managed laparoscopically. Laparoscopy was terminated in eight patients in whom blind-ending cord structures were detected intra-abdominally. An inguinal canal exploration was performed in 10 cases in whom cord structures were seen entering the internal inguinal ring. No viable testis was found, and testicular remnants were excised for histopathologic examination. Four canalicular testes (peeping) were treated with open orchiopexy. Laparoscopy-assisted orchiopexy without vascular ligation was performed in two testes. Fowler-Stephens orchiopexy in single stage was performed in one testicular unit and in two stages in another unit. All patients were discharged on the same day. The testes were normal in size and found in the scrotum after a mean follow-up period of 14 months.Conclusion The laparoscopic findings in NPT had an important influence on treatment decisions. To be able to interpret the definitive surgery relative to the laparoscopic classification, collaborative studies are required. Keywords: laparoscopy, nonpalpable testis, nubbin, orchiopexy, vas deferen

Quantification of Three-Dimensional Cell-Mediated Collagen Remodeling Using Graph Theory

Background: Cell cooperation is a critical event during tissue development. We present the first precise metrics to quantify the interaction between mesenchymal stem cells (MSCs) and extra cellular matrix (ECM). In particular, we describe cooperative collagen alignment process with respect to the spatio-temporal organization and function of mesenchymal stem cells in three dimensions. Methodology/Principal Findings: We defined two precise metrics: Collagen Alignment Index and Cell Dissatisfaction Level, for quantitatively tracking type I collagen and fibrillogenesis remodeling by mesenchymal stem cells over time. Computation of these metrics was based on graph theory and vector calculus. The cells and their three dimensional type I collagen microenvironment were modeled by three dimensional cell-graphs and collagen fiber organization was calculated from gradient vectors. With the enhancement of mesenchymal stem cell differentiation, acceleration through different phases was quantitatively demonstrated. The phases were clustered in a statistically significant manner based on collagen organization, with late phases of remodeling by untreated cells clustering strongly with early phases of remodeling by differentiating cells. The experiments were repeated three times to conclude that the metrics could successfully identify critical phases of collagen remodeling that were dependent upon cooperativity within the cell population. Conclusions/Significance: Definition of early metrics that are able to predict long-term functionality by linking engineere

The Hazar pull-apart along the East Anatolian Fault: Structure and active deformation

Understanding the irregularity of seismic cycles: A case study in Turke

The Hazar pull-apart along the East Anatolian Fault: Structure and active deformation

Understanding the irregularity of seismic cycles: A case study in Turke

Digital microfluidics for reconfigurable antennas

Usage of fluids (microfluidic or otherwise) in antennas provides a conceptually easy reconfiguration mechanism in the aspect of physical alteration. However, a requirement of pumps, valves, etc. for liquid transportation makes the antenna implementations rather impractical for the real-life scenarios. This work reports on the theoretical calculations and experiments conducted to evaluate the electrowetting on dielectric (EWOD) driven digital microfluidics as a reconfiguration mechanism for antennas and RF circuits. © 2014 European Association on Antennas and Propagation

Broadening Intellectual Diversity in Visualization Research Papers

Promoting a wider range of contribution types can facilitate healthy growth of the visualization community, while increasing the intellectual diversity of visualization research papers. In this article, we discuss the importance of contribution types and summarize contribution types that can be meaningful in visualization research. We also propose several concrete next steps we can and should take to ensure a successful launch of the contribution types

Lithium-mediated downregulation of PKB/Akt and cyclin E with growth inhibition in hepatocellular carcinoma cells

We studied in vitro effects of glycogen synthase kinase 3β (GSK3β)-inhibitor lithium on the growth of hepatocellular carcinoma (HCC) cells. Lithium induced strong growth inhibition (>70%) in 75% (n = 9 of 12) of cell lines, apparently independent from the status of major genes that are mutated in HCC including p53, p16INK4a, β-catenin and Axin1. Comparative studies with a growth-sensitive Huh7 and growth-resistant Hep40 cell lines showed that lithium induces growth arrest in Huh7 cells but not in Hep40 cells. Lithium induced the accumulation of N-terminally phosphorylated inactive form of GSK3β with concomitant increase in β-catenin and β-catenin/TCF transcriptional activity in both cell lines. This suggests that lithium-mediated HCC growth inhibition is independent of its well-known stimulatory effect on Wnt-β-catenin signaling. The main differences between Huh7 and Hep40 responses to lithium treatment were observed at the levels PKB/Akt and cyclin E proteins. Lithium induced depletion of both proteins in growth-sensitive Huh7, but not in growth-resistant Hep40 cells. PKB/Akt and Cyclin E are 2 major proteins that are known to be constitutively active in HCC. The targeting of both proteins with lithium may be the main reason why most HCC cells are responsive to lithium-mediated growth inhibition, independent of their p53, retinoblastoma and Wnt-β-catenin pathways. The exploration of molecular mechanisms involved in lithium-mediated growth inhibition in relation with PKB/Akt and cyclin E downregulation may provide new insights for therapy of liver tumors. © 2005 Wiley-Liss, Inc

RESTful API Testing Methodologies: Rationale, Challenges, and Solution Directions

Service-oriented architecture has evolved to be the backbone for large-scale integration between different applications and platforms. This concept has led to today's reality of cloud services. Many of the major business platforms are providing their services to end-users and other companies as well. Companies are crafting ways to allow other businesses fast service integration and to get on board quickly in the market. REST (representational state transfer) has emerged as the standard protocol for implementing and consuming these services, which are called RESTful application programming interfaces (APIs). As the internal details of the RESTful APIs are not completely available during consumption, thorough testing has been a major challenge. Any unprecedented change in the APIs can cause the major failure of service operations, which can cause an organization to face both financial and trust losses. Research efforts have been made to alleviate testing challenges by introducing different frameworks and auto-generating unit test approaches. However, there is still a lack of an overview of the state-of-the-art in RESTful API testing. As such, the objective of this article is to identify, analyze, and synthesize the studies that have been performed related to RESTful APIs' testing methodologies and unit test generation. With this perspective, a systematic literature review (SLR) study was conducted. In total, 16 papers were retrieved and included based on study selection criteria for in-depth analysis. This SLR discusses and categorizes different problems and solutions related to RESTful APIs' testing and unit test generation. 2022 by the authors. Licensee MDPI, Basel, Switzerland.Funding: This research was partially funded by the Norwegian University of Science and Technology, Norway for the support of Open Access fund.Scopus2-s2.0-8512988348

Preparation of Al 2O 3and AlN nanotubes by atomic layer deposition

Al 2O 3 and AlN nanotubes were fabricated by depositing conformal thin films via atomic layer deposition (ALD) on electrospun nylon 66 (PA66) nanofiber templates. Depositions were carried out at 200°C, using trimethylaluminum (TMAl), water (H 2O), and ammonia (NH 3) as the aluminum, oxygen, and nitrogen precursors, respectively. Deposition rates of Al 2O 3 and AlN at this temperature were ∼1.05 and 0.86 Å/cycle. After the depositions, Al 2O 3- and AlN-coated nanofibers were calcinated at 500°C for 2 h in order to remove organic components. Nanotubes were characterized by using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). AlN nanotubes were polycrystalline as determined by high resolution TEM (HR-TEM) and selected area electron diffraction (SAED). TEM images of all the samples reported in this study indicated uniform wall thicknesses. © 2012 Materials Research Society