21 research outputs found
Efecto de los B-glucanos de levaduras y oligoquitosanos sobre los rendimientos productivos en gazapos
El objetivo de este trabajo fue estudiar el efecto de la suplementación con B-glucanos de levaduras y quitosanos sobre los rendimientos productivos de los gazapos
Tubulin cytoskeleton during microsporogenesis in the male-sterile genotype of Allium sativum and fertile Allium ampeloprasum L.
Diversity in Allium ampeloprasum: from small and wild to large and cultivated
Allium ampeloprasum evolved as a complex
of different cyto- and morpho-types widely
distributed either in the wild or domesticated range
of the Mediterranean regions. The assessment of
genetic and phylogenetic relationships between Tunisian
A. ampeloprasum and specimens from different
origins and with variable degree of domestication can
promote conservation and breeding. Minisatellite
M13, microsatellite (GTG)5 and nucleotide sequence
analysis of the internal transcribed spacer region (ITS)
were used to assess DNA polymorphism and genetic
diversity. M13 and (GTG)5 molecular markers efficiently
discriminated A. ampeloprasum gene-pool
from A. sativum. Geographic genetic patterns of
variation of the wild gene-pool were not detected.
However, domesticated A. ampeloprasum (great
headed garlic, kurrat and leek) clustered consistently
within the ampeloprasum group. A. sativum was found
to be closer to A. ampeloprasum than A. fistulosum and
A. schoenoprasum. A high number of single point
mutations (SNPs) was recorded over the ITS1-2 spacer
sequence. Most of these SNPs were heterozygous only
in great headed garlic. It is inferred that heterozygosity
played the major role in promoting great headed garlic
domestication. Thus, great headed garlic adaptation to
horticultural conditions along with its yield trait sizes
are mainly associated to heterozygosity rather than to
polyploidy
MOLECULAR INSIGHT INTO GREAT HEADED GARLIC DOMESTICATION
Allium ampeloprasum evolved as a complex of cyto- and morpho-types widely distributed either in the wild or domesticated range of the Mediterranean regions. The assessment of genetic and hylogenetic relationships between Mediterranean A. ampeloprasum from different ecological niches can provide clues about the origin and domestication of great headed garlic (A . ampeloprasum var Holmense, 2n = 6x = 48). Minisatellite M13, microsatellite (GTG)5 and nucleotide sequence analysis of the internal transcribed spacer region (ITS) were used to assess
DNA polymorphism and genetic diversity. M13 and (GTG)5 molecular markers efficiently discriminated A. ampeloprasum gene-pool from A. sativum. Domesticated A. ampeloprasum (great
headed garlic, kurrat and leek) clustered consistently within the ampeloprasum group. Allium sativum was found to be closer to A. ampeloprasum than A. fistulosum and A. schoenoprasum. A
high number of single point mutations (SNPs) was recorded over the ITS1-2 spacer sequence. Most of great headed garlic SNPs were heterozygous and shared by different specimens of the speciescomplex. All ITS haplotypes were in cis state. It is inferred that heterozygosity played the major role in great headed garlic domestication, adaptation to horticultural conditions along with the increase of yield trait sizes. Thus the switch from a small and wild phenotype to the large and cultivated is mainly associated to heterozygosity rather than to polyploidy