Reversibility of Red blood Cell deformation

The ability of cells to undergo reversible shape changes is often crucial to their survival. For Red Blood Cells (RBCs), irreversible alteration of the cell shape and flexibility often causes anemia. Here we show theoretically that RBCs may react irreversibly to mechanical perturbations because of tensile stress in their cytoskeleton. The transient polymerization of protein fibers inside the cell seen in sickle cell anemia or a transient external force can trigger the formation of a cytoskeleton-free membrane protrusion of micrometer dimensions. The complex relaxation kinetics of the cell shape is shown to be responsible for selecting the final state once the perturbation is removed, thereby controlling the reversibility of the deformation. In some case, tubular protrusion are expected to relax via a peculiar "pearling instability".Comment: 4 pages, 3 figure

Chromosome Oscillations in Mitosis

Successful cell division requires a tight regulation of chromosome motion via the activity of molecular motors. Many of the key players at the origin of the forces generating the movement have been identified, but their spatial and temporal organization remains elusive. The protein complex Kinetochore on the chromosome associates with microtubules emanating from one of the spindle poles and drives the chromosome toward the pole. Chromokinesin motors on the chromosome arms also interact with microtubules, ejecting the chromosome away from the pole. In animal cells, a monooriented chromosome (associated to a single pole) periodically switches between phases of poleward and away from the pole movement[, a behavior tentatively explained so far by the existence of a complex switching mechanism within the kinetochore itself. Here we show that the interplay between the morphology of the mitotic spindle and the collective kinetics of chromokinesins can account for the highly non-linear periodic chromosome motion. Our analysis provides a natural explanation for the origin of chromosome directional instability and for the mechanism by which chromosomes feel their position in space.Comment: http://hogarth.pct.espci.fr/~pierre

Hydro-osmotic instabilities in active membrane tubes

We study a membrane tube with unidirectional ion pumps driving an osmotic pressure difference. A pressure driven peristaltic instability is identified, qualitatively distinct from similar tension-driven Rayleigh type instabilities on membrane tubes. We discuss how this instability could be related to the function and biogenesis of membrane bound organelles, in particular the contractile vacuole complex. The unusually long natural wavelength of this instability is in agreement with that observed in cells

Dynamics of passive and active membrane tubes

Utilising Onsager's variational formulation, we derive dynamical equations for the relaxation of a fluid membrane tube in the limit of small deformation, allowing for a contrast of solvent viscosity across the membrane and variations in surface tension due to membrane incompressibility. We compute the relaxation rates, recovering known results in the case of purely axis-symmetric perturbations and making new predictions for higher order (azimuthal) $m$-modes. We analyse the long and short wavelength limits of these modes by making use of various asymptotic arguments. We incorporate stochastic terms to our dynamical equations suitable to describe both passive thermal forces and non-equilibrium active forces. We derive expressions for the fluctuation amplitudes, an effective temperature associated with active fluctuations, and the power spectral density for both the thermal and active fluctuations. We discuss an experimental assay that might enable measurement of these fluctuations to infer the properties of the active noise. Finally we discuss our results in the context of active membranes more generally and give an overview of some open questions in the field.Comment: 14 pages, 9 figure

Non-equilibrium raft-like membrane domains under continuous recycling

We present a model for the kinetics of spontaneous membrane domain (raft) assembly that includes the effect of membrane recycling ubiquitous in living cells. We show that the domains have a broad power-law distribution with an average radius that scales with the 1/4 power of the domain lifetime when the line tension at the domain edges is large. For biologically reasonable recycling and diffusion rates the average domain radius is in the tens of nm range, consistent with observations. This represents one possible link between signaling (involving rafts) and traffic (recycling) in cells. Finally, we present evidence that suggests that the average raft size may be the same for all scale-free recycling schemes.Comment: 8 pages, 5 figure

Higher order effects in the 16O(d,p)17O^{16}O(d,p)^{17}O and 16O(d,n)17F^{16}O(d,n)^{17}F transfer reactions

Full Coupled Channels Calculations were performed for the $^{16}O(d,n)^{17}F$ and $^{16}O(d,p)^{17}O$ transfer reactions at several deuteron incident energies from $E_{lab}=2.29$ MeV up to 3.27 MeV. A strong polarization effect between the entrance channel and the transfer channels $^{16}O(d,n)^{17}F(1/2^{+},0.495)$ and $^{16}O(d,p)^{17}O(1/2^{+},0.87)$ was observed. This polarization effect had to be taken into account in order to obtain realistic spectroscopic factors from these reactions.Comment: 15 papes, 13 figures, accepted for publication in Phys. Rev.

Force balance and membrane shedding at the Red Blood Cell surface

During the aging of the red-blood cell, or under conditions of extreme echinocytosis, membrane is shed from the cell plasma membrane in the form of nano-vesicles. We propose that this process is the result of the self-adaptation of the membrane surface area to the elastic stress imposed by the spectrin cytoskeleton, via the local buckling of membrane under increasing cytoskeleton stiffness. This model introduces the concept of force balance as a regulatory process at the cell membrane, and quantitatively reproduces the rate of area loss in aging red-blood cells.Comment: 4 pages, 3 figure

Multi-FedLS: a Framework for Cross-Silo Federated Learning Applications on Multi-Cloud Environments

Federated Learning (FL) is a distributed Machine Learning (ML) technique that can benefit from cloud environments while preserving data privacy. We propose Multi-FedLS, a framework that manages multi-cloud resources, reducing execution time and financial costs of Cross-Silo Federated Learning applications by using preemptible VMs, cheaper than on-demand ones but that can be revoked at any time. Our framework encloses four modules: Pre-Scheduling, Initial Mapping, Fault Tolerance, and Dynamic Scheduler. This paper extends our previous work \cite{brum2022sbac} by formally describing the Multi-FedLS resource manager framework and its modules. Experiments were conducted with three Cross-Silo FL applications on CloudLab and a proof-of-concept confirms that Multi-FedLS can be executed on a multi-cloud composed by AWS and GCP, two commercial cloud providers. Results show that the problem of executing Cross-Silo FL applications in multi-cloud environments with preemptible VMs can be efficiently resolved using a mathematical formulation, fault tolerance techniques, and a simple heuristic to choose a new VM in case of revocation.Comment: In review by Journal of Parallel and Distributed Computin

Injector fouling and its impact on engine emissions and spray characteristics in gasoline direct injection engines

In Gasoline Direct Injection engines, direct exposure of the injector to the flame can cause combustion products to accumulate on the nozzle, which can result in increased particulate emissions. This research observes the impact of injector fouling on particulate emissions and the associated injector spray pattern and shows how both can be reversed by utilising fuel detergency. For this purpose multi-hole injectors were deliberately fouled in a four-cylinder test engine with two different base fuels. During a four hour injector fouling cycle particulate numbers (PN) increased by up to two orders of magnitude. The drift could be reversed by switching to a fuel blend that contained a detergent additive. In addition, it was possible to completely avoid any PN increase, when the detergent containing fuel was used from the beginning of the test. Microscopy showed that increased injector fouling coincided with increased particulate emissions. Based on these results a selection of the injectors was installed in a laboratory injection chamber and the spray patterns were investigated with a high speed camera. Injectors corresponding to the largest PN drift produced the thinnest spray jets with the deepest penetration. These factors amplify the risk of wall wetting and provide an explanation for the increase of PN. The positive effect of the detergent was also reflected in the spray pattern analysis, which illustrates the potential benefits of such fuel additives