Cronologia di tracce amatiche: impiego di metodi "classici" ed analisi del DNA

Studio relativo alla possibilità di tipizzare tracce ematiche in relazione all'età delle tracce stesse per polimorfismi classici (antigeni ed enzimi eritrocitari) e del DNA ( polimorfismi individuati dalle sonde pYnh24, pCZ 3.1 e 3'HVR

Esperienza del laboratorio di immunoemogenetica ed identificazione medico-legale in Pavia (1979-1988)

Gli Autori espongono l'esperienza maturata presso il laboratorio di immunoemogenetica ed identificazione medico-legale dell'istituto di Medicina Legale e delle Assicurazioni dell'Università di Pavia negli anni 1979-1988 sottolineando gli aspetti organizzativi e le attività di ricerca, didattica e applicativ

Italian population data for the new ENFSI/EDNAP loci D1S1656, D2S441, D10S1248, D12S391, D22S1045. The GeFI collaborative exercise and concordance study

A collaborative exercise of the ISFG Italian Working Group GeFI was organised to investigate the five new ENFSI/EDNAP miniSTR loci D1S1656, D2S441, D10S1248, D12S391 and D22S1045. Allele frequencies were determined in a sample of 960 individuals collected by the nineteen participating laboratories. The concordance of the genotypes has been evaluated in duplicate experiments, using different kits. No discordant genotypes were revealed for the five ENFSI/EDNAP miniSTR markers. All the labs participating in the collaborative exercise correctly typed the two blind blood stains sent for proficiency testing

Molecular characterisation of a mummified body found in a glacier

A complete genetic profile of a mummified male body that had emerged from the melting ice of an Alpine Glacier was achieved. The body could be dated back to the late 1920s, due to the finding of newspaper cuttings in an internal pocket of the clothes. DNA was recovered from still well preserved internal organs and nail clippings. Autosomal STRs, Y chromosomal STRs and SNPs and mtDNA HV1 region were determined in order to provide a complete genetic survey of the mummified body. In a future perspective, after having collected general information on the possible identity of the body, all these genetic data, and especially the ones from the lineage markers, could be used to get a positive identification

Systematic study on the analytical parameters relevant to achieve reliable STR profiles, as assessed in a multicentre data set

8noA set of 16 amplifications following a D-optimal experimental design was planned using the most commonly selected kit and sequencer utilized in a collaborative exercise of the ISFG Italian Working Group GeFI on the molecular characterization of a depurinated DNA sample. The data were evaluated using STRvalidator in order to describe the occurrence of PCR artifacts such as peak imbalance and allele/ locus drop outs. The aim of the study was to describe the role of the template amount, number of amplification cycles, volume of the PCR reaction in determining the assay sensitivity and the profiling accuracy as measured by peak heights and areas. The results showed that only by selecting an appropriate ratio of template to PCR reaction solution total volume together with an increase in the number of cycles it could be possible to obtain balanced peaks as well as minimal occurrence of other artifacts. These data are useful to model and understand the PCR artifacts occurrence described in the collaborative exercise.reservedmixedMarrubini, G.; Previderè, C.; Sorçaburu-Ciglieri, S.; Grignani, P.; Pitacco, P.; Consoloni, L.; di Cusano, C. Melzi; Fattorini, P.Marrubini, G.; Previderè, C.; Sorçaburu Ciglieri, S.; Grignani, P.; Pitacco, P.; Consoloni, L.; di Cusano, C. Melzi; Fattorini, Paol