Extrusion processing of pure chokeberry (Aronia melanocarpa) pomace: impact on dietary fiber profile and bioactive compounds

The partial substitution of starch with dietary fiber (DF) in extruded ready-to-eat texturized (RTE) cereals has been suggested as a strategy to reduce the high glycemic index of these food products. Here, we study the impact of extrusion processing on pure chokeberry (Aronia melanocarpa) pomace powder (CPP) rich in DF and polyphenols (PP) focusing on the content and profile of the DF fractions, stability of PP, and techno-functional properties of the extrudates. Using a co-rotating twin-screw extruder, different screw speeds were applied to CPP with different water contents (c$_{w}$), which resulted in specific mechanical energies (SME) in the range of 145–222 Whkg$^{-1}$ and material temperatures (T$_{M}$) in the range of 123–155 °C. High molecular weight soluble DF contents slightly increase with increasing thermomechanical stress up to 16.1 ± 0.8 g/100 g dm as compared to CPP (11.5 ± 1.2 g/100 g dm), but total DF (TDF) contents (58.6 ± 0.8 g/100 g dm) did not change. DF structural analysis revealed extrusion-based changes in the portions of pectic polysaccharides (type I rhamnogalacturonan) in the soluble and insoluble DF fractions. Contents of thermolabile anthocyanins decrease linearly with SME and temperature from 1.80 ± 0.09 g/100 g dm in CPP to 0.24 ± 0.06 g/100 g dm (222 Whkg$_{-1}$, 155 °C), but phenolic acids and flavonoids appear to be largely unaffected. Resulting techno-functional (water absorption and water solubility) and physical properties related to the sensory characteristics (expansion, hardness, and color) of pure CPP extrudates support the expectation that granulated CPP extrudates may be a suitable food ingredient rich in DF and PP

Impact of defined thermomechanical treatment on the structure and content of dietary fiber and the stability and bioaccessibility of polyphenols of chokeberry (Aronia melanocarpa) pomace

Dietary fiber is a potential replacement for other ingredients such as starch in reformulated extruded breakfast cereals. Analysis of chokeberry pomace powder revealed a total dietary fiber content of 57.8 ± 2 g/100 g with 76% being insoluble, 20% high molecular soluble and 4% low molecular soluble dietary fiber. The fiber polysaccharide composition was analyzed in detail by using a variety of analytical approaches. Extrusion-like processing conditions were studies in a Closed Cavity Rheometer enabling the application of defined thermal (temperature range 100–160 °C) and mechanical treatments (shear rates between 0.1 s$^{-1}$ and 50 s$^{-1}$) to chokeberry pomace powder. Application of temperatures up to 140 °C irrespective of the mechanical treatment does not remarkably alter dietary fiber structure or content, but reduces the initial content of total polyphenols by about 40% to a final content of 3.3 ± 0.5 g/100 g including 0.63 ± 0.1 g/100 g of anthocyanins, 0.18 ± 0.02 g/100 g of phenolic acids and 0.090 ± 0.007 g/100 g of flavonols, respectively. The retained polyphenols are fully bioaccessible after in vitro digestion, and antioxidant capacity remains unchanged as compared to the untreated pomace powder. Glucose bioaccessibility remains unaffected, whereas glucose content is reduced. It is concluded that chokeberry pomace powder is a good source of dietary fiber with the potential to partially substitute starch in extruded breakfast cereals

Metabolism of Foodborne Heterocyclic Aromatic Amines by <i>Lactobacillus reuteri</i> DSM 20016

The heterocyclic aromatic amine (HAA) 2-amino-1-methyl-6-phenylimidazo­[4,5-<i>b</i>]­pyridine (PhIP) is converted into 7-hydroxy-5-methyl-3-phenyl-6,7,8,9-tetrahydropyrido­[3′,2′:4,5]­imidazo­[1,2-<i>a</i>]­pyrimidin-5-ium chloride (PhIP-M1) via a chemical reaction with 3-hydroxypropionaldehyde or acrolein derived from glycerol by reuterin producing gut bacteria. Because it is unknown whether this reaction also applies to other HAAs, seven foodborne HAAs (2-amino-9<i>H</i>-pyrido­[2,3-<i>b</i>]­indole (AαC), 3-amino-1,4-dimethyl-5<i>H</i>-pyrido­[4,3-<i>b</i>]­indole (Trp-P-1), 2-amino-3-methyl-3<i>H</i>-imidazo­[4,5-<i>f</i>]­quinoline (IQ), 2-amino-3,4-dimethyl-3<i>H</i>-imidazo­[4,5-<i>f</i>]­quinoline (MeIQ), 2-amino-3,8-dimethyl-3<i>H</i>-imidazo­[4,5-<i>f</i>]­quinoxaline (MeIQx), 9<i>H</i>-pyrido­[3,4-<i>b</i>]­indole (norharman), and 1-methyl-9<i>H</i>-pyrido­[3,4-<i>b</i>]­indole (harman)) were anaerobically incubated with <i>Lactobacillus reuteri</i> DSM 20016 in the presence of glycerol. The extent of conversion, as analyzed by HPLC-DAD/FLD, was dependent on both the studied HAAs and the glucose/glycerol ratio, indicating reuterin to be involved in HAA metabolism. Based on HRMS analyses, PhIP-M1-type metabolites were detected for AαC, Trp-P-1, IQ, MeIQ, MeIQx, harman, and norharman. In the case of AαC, this was confirmed by metabolite isolation (AαC-M8, 2,3,4,10-tetrahydro-1<i>H</i>-indolo­[2,3-<i>b</i>]­[1,8]­naphthyridin-2-ol) and one- (¹H) and two-dimensional (HSQC, HMBC, COSY, DOSY) NMR spectroscopy. In addition, based on HRMS and/or NMR spectroscopy, a new type of HAA metabolite, resulting from the reaction with two molecules of 3-hydroxypropionaldehyde or acrolein, is hypothesized for AαC, Trp-P-1, IQ, MeIQ, and MeIQx

Formation of phosphoglycosides in Caenorhabditis elegans: a novel biotransformation pathway.

BACKGROUND: Caenorhabditis elegans (C. elegans) has become a widely used model to explore the effect of food constituents on health as well as on life-span extension. The results imply that besides essential nutrients several flavonoids are able to impact the aging process. What is less investigated is the bioavailability and biotransformation of these compounds in C. elegans. In the present study, we focused on the soy isoflavone genistein and its metabolism in the nematode as a basis for assessing whether this model system mimics the mammalian condition. PRINCIPAL FINDINGS: C. elegans was exposed to 100 µM genistein for 48 hours. The worm homogenate was extracted and analyzed by liquid chromatography (LC). 11 metabolites of genistein were detected and characterized using LC electrospray ionization mass spectrometry. All genistein metabolites formed by C. elegans were found to be sugar conjugates, primarily genistein-O-glucosides. The dominant metabolite was identified as genistein-7-O-phosphoglucoside. Further interesting metabolites include two genistein-di-O-glycosides, a genistein-O-disaccharide as well as a genistein-O-phosphodisaccharide. CONCLUSIONS/SIGNIFICANCE: Our study provides evidence for a novel biotransformation pathway in C. elegans leading to conjugative metabolites which are not known for mammals. The metabolism of genistein in mammals and in C. elegans differs widely which may greatly impact the bioactivity. These differences need to be appropriately taken into consideration when C. elegans is used as a model to assess possible health or aging effects

Effect of potassium fertilization on the contents of antioxidants in three cocktail tomato cultivars

Tomatoes are an important source of health beneficial phytochemicals, which act as antioxidants such as ascorbic acid, phenolic compounds, carotenoids, and tocopherols. The content of antioxidants is influenced, among others, by abiotic stress factors, like the nutritional status. Potassium (K) is a macronutrient, which is essential for several physiological functions in plants, for example, translocation of assimilates, activation of enzymes, maintenance of turgescence, and stomata regulation. The aim of this study was to investigate the effect of increasing K fertilization on the concentration of antioxidants in cocktail tomatoes. Therefore, three tomato cultivars (Primavera, Resi, and Yellow Submarine), grown in an outdoor pot experiment, were fertilized with increasing K doses for two consecutive years. It was confirmed that antioxidants in tomato fruit can be affected by the K regime, but was also shown that other factors may reduce or even inverse those effects when cultivation takes place in an uncontrolled outdoor environment. Most consistent K fertilization effects were found for naringenin, p-coumaric acid and caffeic acid. However, enrichment of tomatoes with antioxidants by K fertilization is cultivar dependent and, therefore, general statements should be avoided