The Drosophila MicroRNA Mir-14 Suppresses Cell Death and Is Required for Normal Fat Metabolism

MicroRNAs (miRNAs) are small regulatory RNAs that are between 21 and 25 nucleotides in length and repress gene function through interactions with target mRNAs 1, 2. The genomes of metazoans encode on the order of several hundred miRNAs [3], but the processes they regulate have been defined for only two in C. elegans4, 5. We searched for new inhibitors of apoptotic cell death by testing existing collections of P element insertion lines for their ability to enhance a small-eye phenotype associated with eye-specific expression of the Drosophila cell death activator Reaper. Here we report the identification of the Drosophila miRNA mir-14 as a cell death suppressor. Loss of mir-14 enhances Reaper-dependent cell death, whereas ectopic expression suppresses cell death induced by multiple stimuli. Animals lacking mir-14 are viable. However, they are stress sensitive and have a reduced lifespan. Mir-14 mutants have elevated levels of the apoptotic effector caspase Drice, suggesting one potential site of action. Mir-14 also regulates fat metabolism. Deletion of mir-14 results in animals with increased levels of triacylglycerol and diacylglycerol, whereas increases in mir-14 copy number have the converse effect. We discuss possible relationships between these phenotypes

On the expected uniform error of geometric Brownian motion approximated by the L\'evy-Ciesielski construction

It is known that the Brownian bridge or L\'evy-Ciesielski construction of Brownian paths almost surely converges uniformly to the true Brownian path. In the present article the focus is on the error. In particular, we show for geometric Brownian motion that at level $N$, at which there are $d=2^N$ points evaluated on the Brownian path, the expected uniform error has an upper bound of order $\mathcal{O}(\sqrt{N/2^N})$, or equivalently, $\mathcal{O}(\sqrt{\ln d/d})$. This upper bound matches the known order for the expected uniform error of the standard Brownian motion. We apply the result to an option pricing example

A Study of H2 Emission in Three Bipolar Proto-Planetary Nebulae: IRAS 16594-4656, Hen 3-401, and Rob 22

We have carried out a spatial-kinematical study of three proto-planetary nebulae, IRAS 16594-4656, Hen 3-401, and Rob 22. High-resolution H2 images were obtained with NICMOS on the HST and high-resolution spectra were obtained with the Phoenix spectrograph on Gemini-South. IRAS 16594-4656 shows a "peanut-shaped" bipolar structure with H2 emission from the walls and from two pairs of more distant, point-symmetric faint blobs. The velocity structure shows the polar axis to be in the plane of the sky, contrary to the impression given by the more complex visual image and the visibility of the central star, with an ellipsoidal velocity structure. Hen 3-401 shows the H2 emission coming from the walls of the very elongated, open-ended lobes seen in visible light, along with a possible small disk around the star. The bipolar lobes appear to be tilted 10-15 deg with respect to the plane of the sky and their kinematics display a Hubble-like flow. In Rob 22, the H2 appears in the form of an "S" shape, approximately tracing out the similar pattern seen in the visible. H2 is especially seen at the ends of the lobes and at two opposite regions close to the unseen central star. The axis of the lobes is nearly in the plane of the sky. Expansion ages of the lobes are calculated to be approximately 1600 yr (IRAS 16594-4656), 1100 yr (Hen 3-401), and 640 yr (Rob 22), based upon approximate distances

Identification of a component of Drosophila polar granules

Information necessary for the formation of pole cells, precursors of the germ line, is provided maternally and localized to the posterior pole of the Drosophila egg. The maternal origin and posterior localization of polar granules suggest that they may be associated with pole cell determinants. We have generated an antibody (Mab46F11) against polar granules. In oocytes and early embryos, the Mab46F11 antigen is sharply localized to the posterior embryonic pole. In pole cells, it becomes associated with nuclear bodies within, and nuage around, the nucleus. Immunoreactivity remains associated with cells of the germ line throughout the life cycle of both males and females. This antibody recognizes a 72-74 X 10^(3) Mr protein and is useful both as a pole lineage marker and in biochemical studies of polar granules

High-Resolution Near-Infrared Imaging and Polarimetry of Four Proto-Planetary Nebulae

High-resolution near-infrared HST NICMOS (F160W, F222M) images and polarization (2 um) observations were made of four bipolar proto-planetary nebulae (PPNs): IRAS 17150-3224, IRAS 17441-2411, IRAS 17245-3951, and IRAS 16594-4656. The first three of these are viewed nearly edge-on, and for the first time the central stars in them are seen. Color maps reveal a reddened torus between the bipolar lobes in the edge-on cases, with bluer lobes. The polarization values are high, with maximum values ranging from 40 to 80%. The polarization patterns are basically centrosymmetric, with some deviations in the low polarization equatorial regions. For IRAS 17150-3224, circumstellar arcs are seen at 1.6 um, along with a newly-discovered loop in the equatorial region. Bright caps are seen at the end of the lobes, indicating that they are not open-ended. A distinct point-symmetric pattern is seen in the strengths of the polarization vectors, especially in IRAS 17150-3224. HST NICMOS observations provide a valuable complement to the WFPC2 visible images in deriving the basic structure of bipolar PPNs.Comment: 20 pages, 12 figures, accepted for publication in Astronomical Journal higher resolution figures are available on http://katherine.as.arizona.edu/~ksu/cv/su_nicmos7840.ps.g

THE IMPACT OF SWINE PRODUCTION ON LAND VALUES IN ILLINOIS

Based on a spatiotemporal hedonic farmland price model and county-level data in Illinois from 1979 to 1999, we examined the impact of swine production on farmland values. Our results show that, in addition to the conventional determinants of farmland values, an increase in swine production intensity has a negative relationship with farmland values while an increase in swine operation scale had a positive association with farmland values at the county level in Illinois. We also estimate the impact of changes in the Illinois swine industry over the period 1980-1999 on farmland values at the state level and find that changes in swine inventory and scale of swine operations have led to changes in farmland prices from $-10.56 to$62.96 per acre. In general, the changes in Illinois swine industry increase farmland values in Illinois.Land Economics/Use,

The small quinolone derived compound HT61 enhances the effect of tobramycin against Pseudomonas aeruginosa in vitro and in vivo.

HT61 is a small quinolone-derived compound previously demonstrated to exhibit bactericidal activity against gram-positive bacteria including methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). When combined with the classical antibiotics and antiseptics neomycin, gentamicin, mupirocin and chlorhexidine, HT61 demonstrated synergistic bactericidal activity against both MSSA and MRSA infections in vitro. In this study, we investigated the individual antimicrobial activity of HT61 alongside its capability to increase the efficacy of tobramycin against both a tobramycin sensitive laboratory reference strain (PAO1) and tobramycin resistant clinical isolates (RP73, NN2) of the gram-negative bacteria Pseudomonas aeruginosa (P. aeruginosa). Using broth microdilution methods, the MICs of HT61 against all strains were assessed, as well as the effect of HT61 in combination with tobramycin using both the chequerboard method and bacterial time-kill assays. A murine model of pulmonary infection was also used to evaluate the combination therapy of tobramycin and HT61 in vivo. In these studies, we demonstrated significant synergism between HT61 and Tobramycin against the tobramycin resistant P. aeruginosa strains RP73 and NN2, whilst an additive/intermediate effect was observed for P. aeruginosa strain PA01 which was further confirmed using bacterial time kill analysis. In addition, the enhancement of tobramycin by HT61 was also evident in in vitro assays of biofilm eradication. Finally, in vivo studies revealed analogous effects to those observed in vitro with HT61 when administered in combination with tobramycin against each of the three P. aeruginosa strains at the highest tested dose (10 mg/kg)