Evolution of virulence in opportunistic pathogens: generalism, plasticity, and control

Standard virulence evolution theory assumes that virulence factors are maintained because they aid parasitic exploitation, increasing growth within and/or transmission between hosts. An increasing number of studies now demonstrate that many opportunistic pathogens (OPs) do not conform to these assumptions, with virulence factors maintained instead because of advantages in non-parasitic contexts. Here we review virulence evolution theory in the context of OPs and highlight the importance of incorporating environments outside a focal virulence site. We illustrate that virulence selection is constrained by correlations between these external and focal settings and pinpoint drivers of key environmental correlations, with a focus on generalist strategies and phenotypic plasticity. We end with a summary of key theoretical and empirical challenges to be met for a fuller understanding of OPs

Polarimetric variations of binary stars. II. Numerical simulations for circular and eccentric binaries in Mie scattering envelopes

We present numerical simulations of the periodic polarimetric variations produced by a binary star placed at the center of an empty spherical cavity inside a circumbinary ellipsoidal and optically thin envelope made of dust grains. Mie single-scattering is considered along with pre- and post-scattering extinction factors which produce a time-varying optical depth and affect the morphology of the periodic variations. We are interested in the effects that various parameters will have on the average polarization, the amplitude of the polarimetric variations, and the morphology of the variability. We show that the absolute amplitudes of the variations are smaller for Mie scattering than for Thomson scattering. Among the four grain types that we have studied, the highest polarizations are produced by grains with sizes in the range 0.1-0.2 micron. In general, the variations are seen twice per orbit. In some cases, because spherical dust grains have an asymmetric scattering function, the polarimetric curves produced also show variations seen once per orbit. Circumstellar disks produce polarimetric variations of greater amplitude than circumbinary envelopes. Another goal of these simulations is to see if the 1978 BME (Brown, McLean, & Emslie, ApJ, 68, 415) formalism, which uses a Fourier analysis of the polarimetric variations to find the orbital inclination for Thomson-scattering envelopes, can still be used for Mie scattering. We find that this is the case, if the amplitude of the variations is sufficient and the true inclinations is i_true > 45 deg. For eccentric orbits, the first-order coefficients of the Fourier fit, instead of second-order ones, can be used to find almost all inclinations.Comment: 23 pages, 5 figures, to be published in Astronomical Journa

The coevolution of toxin and antitoxin genes drives the dynamics of bacterial addiction complexes and intragenomic conflict

Bacterial genomes commonly contain ‘addiction’ gene complexes that code for both a toxin and a corresponding antitoxin. As long as both genes are expressed, cells carrying the complex can remain healthy. However, loss of the complex (including segregational loss in daughter cells) can entail death of the cell. We develop a theoretical model to explore a number of evolutionary puzzles posed by toxin–antitoxin (TA) population biology. We first extend earlier results demonstrating that TA complexes can spread on plasmids, as an adaptation to plasmid competition in spatially structured environments, and highlight the role of kin selection. We then considered the emergence of TA complexes on plasmids from previously unlinked toxin and antitoxin genes. We find that one of these traits must offer at least initially a direct advantage in some but not all environments encountered by the evolving plasmid population. Finally, our study predicts non-transitive ‘rock-paper-scissors’ dynamics to be a feature of intragenomic conflict mediated by TA complexes. Intragenomic conflict could be sufficient to select deleterious genes on chromosomes and helps to explain the previously perplexing observation that many TA genes are found on bacterial chromosomes

Synergy and Group Size in Microbial Cooperation

Microbes produce many molecules that are important for their growth and development, and the consumption of these secretions by nonproducers has recently become an important paradigm in microbial social evolution. Though the production of these public goods molecules has been studied intensely, little is known of how the benefits accrued and costs incurred depend on the quantity of public good molecules produced. We focus here on the relationship between the shape of the benefit curve and cellular density with a model assuming three types of benefit functions: diminishing, accelerating, and sigmoidal (accelerating then diminishing). We classify the latter two as being synergistic and argue that sigmoidal curves are common in microbial systems. Synergistic benefit curves interact with group sizes to give very different expected evolutionary dynamics. In particular, we show that whether or not and to what extent microbes evolve to produce public goods depends strongly on group size. We show that synergy can create an “evolutionary trap” which can stymie the establishment and maintenance of cooperation. By allowing density dependent regulation of production (quorum sensing), we show how this trap may be avoided. We discuss the implications of our results for experimental design

Electroacoustic oscillations in the LEP SC. cavities

The LEP superconducting cavities have been plagued by electroacoustic oscillations. Tests have been done to eliminate these by a special feed-back loop in the tuning circuit as well as a feed-forward path, but they could only be eliminated safely up to the design field by running the cavities close to tune neglecting beam-loading compensation. This technique proved successful during the first LEP2 test run at 70 GeV. The mechanism and essential parameters driving these oscillations have been analysed as well as the corresponding stronger loading of the power coupler