62 research outputs found
Characterization of Macrophages and Osteoclasts in the Osteosarcoma Tumor Microenvironment at Diagnosis: New Perspective for Osteosarcoma Treatment?
Biological and histopathological techniques identified osteoclasts and macrophages as targets of zoledronic acid (ZA), a therapeutic agent that was detrimental for patients in the French OS2006 trial. Conventional and multiplex immunohistochemistry of microenvironmental and OS cells were performed on biopsies of 124 OS2006 patients and 17 surgical (âOSNewâ) biopsies respectively. CSF-1R (common osteoclast/macrophage progenitor) and TRAP (osteoclast activity) levels in serum of 108 patients were correlated to response to chemotherapy and to prognosis. TRAP levels at surgery and at the end of the protocol were significantly lower in ZA+ than ZAâ patients (padj = 0.0011; 0.0132). For ZA+-patients, an increase in the CSF-1R level between diagnosis and surgery and a high TRAP level in the serum at biopsy were associated with a better response to chemotherapy (p = 0.0091; p = 0.0251). At diagnosis, high CD163+ was associated with good prognosis, while low TRAP activity was associated with better overall survival in ZAâ patients only. Multiplex immunohistochemistry demonstrated remarkable bipotent CD68+/CD163+ macrophages, homogeneously distributed throughout OS regions, aside osteoclasts (CD68+/CD163â) mostly residing in osteolytic territories and osteoid-matrix-associated CD68â/CD163+ macrophages. We demonstrate that ZA not only acts on harmful osteoclasts but also on protective macrophages, and hypothesize that the bipotent CD68+/CD163+ macrophages might present novel therapeutic targets
Une reevaluation de la methode d'incorporation de H14CO3- pour mesurer la nitrification autotrophe et son application pour estimer des biomasses de bacteries nitrifiantes
SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Variations spatiales et saisonniÚres des micro-organismes et des particules au cours de cycles de marées
info:eu-repo/semantics/nonPublishe
Estimation des vitesses de chutes des particules dans lâestuaire de la Seine
info:eu-repo/semantics/nonPublishe
Data mining and knowledge discovery based on dissolved oxygen concentrations recorded continuously: the Zenne river case study in Central Belgium
info:eu-repo/semantics/publishe
Caractérisation des apports de matiÚre organique, nutriments et micro-organismes par les rejets d'eaux usées
Etude réalisée dans le cadre du programme interdisciplinaire de recherche PIREN-Seine (CNRS)info:eu-repo/semantics/publishe
Bilans dâoxygĂšne et de carbone dans lâestuaire amont de la Seine: Rapport final
info:eu-repo/semantics/publishe
Effect of intense rainfall events on metallic and particulate pollutant fluxes in a small urban watercourse
info:eu-repo/semantics/publishe
Mesures in situ des vitesses de chute des constituants particulaires dans lâestuaire de la Seine
info:eu-repo/semantics/nonPublishe
- âŠ