Villából kórházat? A József Attiláról elnevezett kórház története

<p>Preoperative difficulty, expected and actual postoperative improvement on the Catquest-9SF items by functional characteristics (N = 174)^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169844#t004fn001" target="_blank">*</a>}.</p

Distribution of MOS-HIV scale scores.

<p>Distribution of MOS-HIV scale scores.</p

Known group validity of the MOS-HIV scales for different CD4 counts.

<p>Known group validity of the MOS-HIV scales for different CD4 counts.</p

Convergent validity and discriminant validity of the MOS-HIV.

<p>Convergent validity and discriminant validity of the MOS-HIV.</p

Distribution of background characteristics.

<p>Distribution of background characteristics.</p

Internal reliability and inter-correlations of the MOS-HIV scales.

<p>Internal reliability and inter-correlations of the MOS-HIV scales.</p

Standardized estimates of factor loading for the hypothesized model.

<p>Standardized estimates of factor loading for the hypothesized model.</p

A Novel Vector-Based Method for Exclusive Overexpression of Star-Form MicroRNAs

<div><p>The roles of microRNAs (miRNAs) as important regulators of gene expression have been studied intensively. Although most of these investigations have involved the highly expressed form of the two mature miRNA species, increasing evidence points to essential roles for star-form microRNAs (miRNA*), which are usually expressed at much lower levels. Owing to the nature of miRNA biogenesis, it is challenging to use plasmids containing miRNA coding sequences for gain-of-function experiments concerning the roles of microRNA* species. Synthetic microRNA mimics could introduce specific miRNA* species into cells, but this transient overexpression system has many shortcomings. Here, we report that specific miRNA* species can be overexpressed by introducing artificially designed stem-loop sequences into short hairpin RNA (shRNA) overexpression vectors. By our prototypic plasmid, designed to overexpress hsa-miR-146b-3p, we successfully expressed high levels of hsa-miR-146b-3p without detectable change of hsa-miR-146b-5p. Functional analysis involving luciferase reporter assays showed that, like natural miRNAs, the overexpressed hsa-miR-146b-3p inhibited target gene expression by 3′UTR seed pairing. Our demonstration that this method could overexpress two other miRNAs suggests that the approach should be broadly applicable. Our novel strategy opens the way for exclusively stable overexpression of miRNA* species and analyzing their unique functions both <em>in vitro</em> and <em>in vivo</em>.</p> </div

The structural equation modeling of the factors that influence QOL among Chinese MSM.

<p>Path diagram illustrating the relationships between demographic and social support factors and QOL in Chinese MSM. Social support had the greatest impact on QOL, and demographic characteristics appeared to influence the likelihood of receiving social support.</p