11 research outputs found
Nano-bio-tribological analysis of the synovial fluid: role of the molecular assemblies
International audienc
Structural and pathological analysis of healthy and pathological synovial fluids
International audienc
Role of the biomolecular interactions in the structure and tribological properties of synovial fluid
International audienc
Correlation between nanomechanical and tribological properties of healthy and pathological synovial fluids reconstituted from synoviocyte cultures
International audienc
Analysis of the tribological performances of Phospholipid Molecular Assemblies of the biological lubricants
http://www-lpmcn.univ-lyon1.fr/workshop_biolub/index.htm
Ferritin surplus in mouse spleen 14 months after intravenous injection of iron oxide nanoparticles at clinical dose
International audienc
Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances
The quality of the lubricant between cartilaginous joint surfaces impacts the joint’s mechanistic properties. In this study, we define the biochemical, ultrastructural, and tribological signatures of synovial fluids (SF) from patients with degenerative (osteoarthritis-OA) or inflammatory (rheumatoid arthritis-RA) joint pathologies in comparison with SF from healthy subjects. Phospholipid (PL) concentration in SF increased in pathological contexts, but the proportion PL relative to the overall lipids decreased. Subtle changes in PL chain composition were attributed to the inflammatory state. Transmission electron microscopy showed the occurrence of large multilamellar synovial extracellular vesicles (EV) filled with glycoprotein gel in healthy subjects. Synovial extracellular vesicle structure was altered in SF from OA and RA patients. RA samples systematically showed lower viscosity than healthy samples under a hydrodynamic lubricating regimen whereas OA samples showed higher viscosity. In turn, under a boundary regimen, cartilage surfaces in both pathological situations showed high wear and friction coefficients. Thus, we found a difference in the biochemical, tribological, and ultrastructural properties of synovial fluid in healthy people and patients with osteoarthritis and arthritis of the joints, and that large, multilamellar vesicles are essential for good boundary lubrication by ensuring a ball-bearing effect and limiting the destruction of lipid layers at the cartilage surface