855 research outputs found
Immunisation against gonadotrophin-releasing hormone (GnRH) reduces agonistic behaviours in male rangeland goats
Rangeland goat bucks were used to evaluate the efficacy of a commercially available anti-gonadotrophin-releasing hormone vaccine, Improvac (Zoetis Australia, West Ryde, NSW, Australia). The hypothesis tested was that immunisation would suppress testosterone secretion by the testis and agonistic behaviour between male goats. We also compared intervals of 2 and 4 weeks between primary and booster immunisations and monitored responses over a 2-month period. The 45 goats were split into three groups (n = 15): one group receiving the vaccination booster on Day 14, one group receiving the vaccination booster on Day 28, and the Control group receiving sterile saline injections. Body mass, body condition score and scrotal circumference were measured fortnightly, and blood was collected at 2-week intervals and analysed for testosterone concentration. Behavioural interaction tests of 2-min duration were also conducted fortnightly. There was a significant decrease in paired testicular circumference (P < 0.05) and testosterone concentration (P < 0.01) in both vaccination groups by the end of the experiment at Day 60, compared with the Control group. Agonistic interactions measured at Day 60 were significantly reduced in both vaccination groups (P < 0.05) compared with the Control group. These results support the efficacy of Improvac in reducing agonistic behaviours in rangeland goat bucks and suggest that the use of the vaccine may assist in reducing social stress and possible injury in groups of confined male goats
Remote identification of sheep with flystrike using behavioural observations
Flystrike is a major problem affecting sheep in Australia. Identification of &lsquo;flystruck&rsquo; individuals is crucial for treatment; but requires labour-intensive physical examination. As the industry moves toward more low-input systems; there is a need for remote methods to identify flystruck individuals. The aim of this study was to investigate the behaviour of sheep with breech flystrike within a paddock setting. Video footage of sixteen Merino sheep; eight later confirmed with flystrike and eight without; was collected as they moved freely within the paddock with conspecifics. Quantitative behavioural measurements and a qualitative behavioural assessment (QBA) were conducted and compared to their breech conditions (i.e., faecal/urine staining; flystrike severity). Both qualitative and quantitative assessments indicated behavioural differences between flystruck and non-flystruck animals. Flystruck sheep had a behavioural profile characterised by restless behaviour; abnormal postures and reduced grazing time (<i>p</i> &lt; 0.05). Furthermore; flystruck sheep were scored to have a more &lsquo;exhausted/irritated&rsquo; demeanour using QBA (<i>p</i> &lt; 0.05). The behavioural responses also corresponded to the flystrike severity scores and condition of the breech area. We conclude that remotely assessed behaviour of flystruck sheep diverges markedly from non-flystruck sheep; and thus could be a low-input method for identifying and treating affected animals
Animal welfare indicators for sheep during sea transport: The effect of voyage day and time of day
Ensuring the well-being of animals during transport is becoming an increasingly important societal concern. The Australian livestock export industry recognises the need for comprehensive monitoring and reporting on animal welfare during sea transport. It is predicted that pen-side assessments of sheep can be used to monitor environmental conditions, resource access, and animal health and behavioural outcomes throughout a sea voyage. Pen-side assessments by observation are non-invasive and practical to apply in an industry setting. This study monitored sheep using a pilot list of welfare indicators during two sea voyages from Australia to the Middle East, in contrasting seasons. Sheep behaviour, environment and resources were recorded three times daily via pen-side observations of six pens of Merino wethers (castrated males), repeated over three decks for each voyage. Behavioural outcomes were examined for the effect of sampling frequency on group assessments. The number of behavioural measures were reduced via Principal Component (PC) analysis. The primary three PC factors were tested against the time of sampling and pen location after accounting for the effect of environmental- and resource-based predictor variables. PC 1 (24.0 % of the total variance) described activity levels, with sheep on Voyage B being more active in the morning and resting or recumbent in the middle of the afternoon and evening. PC 2 (14.7 %) reflected heat responses with the majority of the variation in these data accounted for by changes in Wet Bulb Globe Temperature (WBGT) and manure pad moisture. The heat responses described by PC 2 also varied by voyage day (p < 0.001) and time point (p < 0.001). PC 3 scores (9.5 %) reflected flight distances and feeding behaviour and strongly correlated to WBGT and pellet consumption per head per day. Feeding behaviour generally became more competitive, and flight distances reduced as both voyages progressed. Results indicate that a comprehensive welfare monitoring protocol requires repeated daily sampling throughout a voyage. The findings of this study are pertinent for developing a sampling strategy to assess sheep welfare during sea transport
On the Nagaoka polaron in the t-J model
It is widely believed that a single hole in the two (or three) dimensional
t-J model, for sufficiently small exchange coupling J, creates a ferromagnetic
bubble around itself, a finite J remnant of the ferromagnetic groundstate at
J=0 (the infinite U Hubbard model), first established by Nagaoka. We
investigate this phenomenon in two dimensions using the density matrix
renormalization group, for system sizes up to 9x9. We find that the polaron
forms for J/t<0.02-0.03 (a somewhat larger value than estimated previously).
Although finite-size effects appear large, our data seems consistent with the
expected 1.1(J/t)^{-1/4} variation of polarion radius. We also test the
Brinkman-Rice model of non-retracing paths in a Neel background, showing that
it is quite accurate, at larger J. Results are also presented in the case where
the
Heisenberg interaction is dropped (the t-J^z model). Finally we discuss a
"dressed polaron" picture in which the hole propagates freely inside a finite
region but makes only self-retracing excursions outside this region.Comment: 7 pages, 9 encapsulated figure
The contribution of qualitative behavioural assessment to appraisal of livestock welfare
Animal welfare is increasingly important for the Australian livestock industries, to maintain social licence to practice as well as ensuring market share overseas. Improvement of animal welfare in the livestock industries requires several important key steps. Paramount among these, objective measures are needed for welfare assessment that will enable comparison and contrast of welfare implications of husbandry procedures or housing options. Such measures need to be versatile (can be applied under a wide range of on- and off-farm situations), relevant (reveal aspects of the animal’s affective or physiological state that is relevant to their welfare), reliable (can be repeated with confidence in the results), relatively economic to apply, and they need to have broad acceptance by all stakeholders. Qualitative Behavioural Assessment (QBA) is an integrated measure that characterises behaviour as a dynamic, expressive body language. QBA is a versatile tool requiring little specialist equipment suiting application to in situ assessments that enables comparative, hypothesis-driven evaluation of various industry-relevant practices. QBA is being increasingly used as part of animal welfare assessments in Europe, and although most other welfare assessment methods record ‘problems’ (e.g. lameness, injury scores, and so on), QBA can capture positive aspects of animal welfare (e.g. positively engaged with their environment, playfulness). In this viewpoint, we review the outcomes of recent QBA studies and discuss the potential application of QBA, in combination with other methods, as a welfare assessment tool for the Australian livestock industries
Decreased lung ischemia-reperfusion injury in rats after preoperative administration of cyclosporine and tacrolimus
AbstractObjectives: Calcineurin inhibitors reduce experimental reperfusion injury in the liver, brain, heart, kidney, and small bowel. These studies were undertaken to determine whether these agents are similarly protective against lung ischemia-reperfusion injury. Methods: Left lungs of male rats were rendered ischemic for 90 minutes and reperfused for as long as 4 hours. Treated animals received cyclosporine A (INN: ciclosporin; 1 or 5 mg/kg) or tacrolimus (0.2 mg/kg) 6 hours before ischemia, at reperfusion, or 2 hours after reperfusion. Injury was quantitated in terms of tissue polymorphonuclear leukocyte accumulation (myeloperoxidase content), vascular permeability (iodine 125-labeled bovine serum albumin extravasation), and bronchoalveolar lavage leukocyte content. Separate tissue samples were processed for nuclear protein and cytokine messenger RNA. Results: Treatment with cyclosporine (5 mg/kg) or tacrolimus (0.2 mg/kg) 6 hours before reperfusion reduced lung vascular permeability by 54% and 56% relative to control animals (P <.03). The protective effects of cyclosporine and tacrolimus treatment before reperfusion correlated with 42% and 43% reductions in tissue polymorphonuclear leukocyte (myeloperoxidase) content (P <.008) and marked reductions in bronchoalveolar lavage leukocyte accumulation (P <.01). Administration of cyclosporine or tacrolimus at the time of reperfusion or 2 hours into the reperfusion period offered little or no protection. Animals treated before reperfusion also demonstrated marked reductions in nuclear factor κB activation and expression of proinflammatory cytokine messenger RNA. Conclusion: Cyclosporine and tacrolimus treatment before reperfusion was protective against lung ischemia-reperfusion injury in rats. The mechanism of these protective effects may involve the inhibition of nuclear factor κB, a central transcription factor mediating inflammatory injury. The decreased expression of cytokine messenger RNA indicates that both cyclosporine and tacrolimus may exert their protective effects at the pretranscriptional level.J Thorac Cardiovasc Surg 2002;123:756-6
Review of livestock welfare indicators relevant for the Australian live export industry
Animal welfare is an important issue for the live export industry (LEI), in terms of economic returns, community attitudes and international socio-political relations. Mortality has traditionally been the main welfare measure recorded within the LEI; however, high mortality incidents are usually acted upon after adverse events occur, reducing the scope for proactive welfare enhancement. We reviewed 71 potential animal welfare measures, identifying those measures that would be appropriate for use throughout the LEI for feeder and slaughter livestock species, and categorised these as animal-, environment- and resource-based. We divided the live export supply chain into three sectors: (1) Australian facilities, (2) vessel and (3) destination country facilities. After reviewing the relevant regulations for each sector of the industry, we identified 38 (sector 1), 35 (sector 2) and 26 (sector 3) measures already being collected under current practice. These could be used to form a ‘welfare information dashboard’: a LEI-specific online interface for collecting data that could contribute towards standardised industry reporting. We identified another 20, 25 and 28 measures that are relevant to each LEI sector (sectors 1, 2, 3, respectively), and that could be developed and integrated into a benchmarking system in the future
Oxidation and fragmentation of plastics in a changing environment; from UV-radiation to biological degradation
Understanding the fate of plastics in the environment is of critical importance for the quantitative assessment of the biological impacts of plastic waste. Specially, there is a need to analyze in more detail the reputed longevity of plastics in the context of plastic degradation through oxidation and fragmentation reactions. Photo-oxidation of plastic debris by solar UV radiation (UVR) makes material prone to subsequent fragmentation. The fragments generated following oxidation and subsequent exposure to mechanical stresses include secondary micro- or nanoparticles, an emerging class of pollutants. The paper discusses the UV-driven photo-oxidation process, identifying relevant knowledge gaps and uncertainties. Serious gaps in knowledge exist concerning the wavelength sensitivity and the dose-response of the photo-fragmentation process. Given the heterogeneity of natural UV irradiance varying from no exposure in sediments to full UV exposure of floating, beach litter or air-borne plastics, it is argued that the rates of UV-driven degradation/fragmentation will also vary dramatically between different locations and environmental niches. Biological phenomena such as biofouling will further modulate the exposure of plastics to UV radiation, while potentially also contributing to degradation and/or fragmentation of plastics independent of solar UVR. Reductions in solar UVR in many regions, consequent to the implementation of the Montreal Protocol and its Amendments for protecting stratospheric ozone, will have consequences for global UV-driven plastic degradation in a heterogeneous manner across different geographic and environmental zones. The interacting effects of global warming, stratospheric ozone and UV radiation are projected to increase UV irradiance at the surface in localized areas, mainly because of decreased cloud cover. Given the complexity and uncertainty of future environmental conditions, this currently precludes reliable quantitative predictions of plastic persistence on a global scale
Single nucleotide polymorphisms in the bovine leptin gene and their association with carcass and efficiency traits, and endocrine profiles, in female Angus cows
One hundred and fifty female Angus cattle were genotyped for the bovine leptin gene SNPs UASMS1, UASMS2, E2FB and E2JW. Net Feed Intake (NFI) Estimated Breeding Values (EBVs) and E2JW SNP data was also acquired from 169 Angus cattle that originated from Trangie Research Station, NSW, and were selected for a divergence in feed efficiency. The E2JW SNP was associated with NFI, NFI EBV and P8 fatness. The UASMS1 and UASMS2 SNPs were associated with circulating leptin concentrations. These particular associations have not been reported previously but similar associations have reported in North American studies. The inconsistent associations suggest that these SNPs are not good candidates for marker-assisted selection for NFI. Also, the investigation of associations with endocrine profiles that reflect body composition such as leptin, requires genotyping of a larger number of Australian cattle than was possible in this experiment
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