Influence of photoperiod and protein diet on growth hormone secretion in rams.

International audienc

Influence of photoperiod and protein diet on growth hormone secretion in rams.

International audienc

Effets des hormones hypothalamiques (GRF, TRH, somatostatine) et du facteur de croissance (IGF-1) sur la secretion de la GH par des cultures d'hypophyse d'agneau male pubere

26 ref. 5 graph.International audienc

Effets des hormones hypothalamiques (GRF, TRH, somatostatine) et du facteur de croissance (IGF-1) sur la secretion de la GH par des cultures d'hypophyse d'agneau male pubere

National audienc

Purification of chinook salmon (Oncorhynchus tshawytscha) GH for receptor study

A method for the purification of chinook Salmon (Oncorhynchus tshawytscha) GH, which retains its biological activity, is described. The biological activity was investigated with an established radioreceptor assay using liver membranes from pregnant rabbits and bovine GH as standard and labelled hormone. The enrichment of the preparation was checked with electrophoresis (SDS-PAGE). Extraction and further steps were carried out using low molarity alkaline buffer (pH 8–10, M = 100 mM). Three chromatography steps were performed (Concanavalin-A sepharose, Bio-gel P60, DEAE). Ion exchange chromatography was performed under isocratic conditions (using a 50 cm column). Two isoforms (sGH1 and sGH2) were isolated. The purification yield is 0.7% compared to lyophilized pituitaries. The molecule is homogeneous in SDS-PAGE. Contamination by prolactin, gonadotrophin and corticotrophin is negligible (< 0.5%). It could be demonstrated that the biological activity of the preparation is maintained since this preparation stimulates the growth of juvenile trout (Salmo gairdneri) and binds specifically (35%) to trout liver membranes

Reevaluation de l'activite lipolytique de l'hormone de croissance vis a vis de l'adipocyte de lapin.

International audienc

Effets du GRF 1-29 chez l'agneau normal et hypotrophique

National audienc

Phylogenetic Trees and Networks Reduce to Phylogenies on Binary States: Does It Furnish an Explanation to the Robustness of Phylogenetic Trees against Lateral Transfers?

International audienc

Effects of experimental cryptorchidism and subsequent orchidopexy on seminiferous tubule functions in the lamb

International audienc

Caracteristiques de la reponse de la GH a l'administration de GRF chez l'agneau

32 ref.International audienc