Immobilization of anti-aflatoxin B1 antibody by UV polymerization of aniline and aflatoxin B1 detection via electrochemical impedance spectroscopy

WOS: 000311817900004PubMed ID: 22947025In the study, we investigated the practicality of the UV polymerization of aniline for anti-aflatoxin B1 antibody immobilization, and utilization of the resulting biosensor in the impedimetric determination of aflatoxin B1. The anti-aflatoxin B 1 antibody was physically immobilized on gold electrodes by UV polymerization of aniline at a fixed wavelength. The biosensor was based on specific interaction anti-aflatoxin B1 - aflatoxin B1 recognition and investigation of this recognition event by electrochemical impedance spectroscopy. A calibration curve was obtained in a linear detection range 1-20 ng/mL aflatoxin B1. Finally, the biosensor was applied to analysis of a real food sample.TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106 T 367]; EBBILTEM (Ege University of Research and Application Center of Science and Technology)Ege University [2007 BBIL 013]Financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 106 T 367) and EBBILTEM (Ege University of Research and Application Center of Science and Technology, Project number: 2007 BBIL 013) is gratefully acknowledged

Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles

WOS: 000291178200013PubMed ID: 21420290The present work describes the construction and application of a new DNA biosensor for detection of aflatoxin M1. In order to immobilize a thiol-modified single stranded DNA (ss-HSDNA) probe that specifically bound aflatoxin M1, a self-assembled monolayer of cysteamine and gold nanoparticles on the SAM were prepared on gold electrodes, layer-by-layer. The assembly processes of cysteamine, gold nanoparticles, and ss-HSDNA were monitored with the help of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)] solution was used as a redox probe for electrochemical measurements. The biosensor provided a linear response to aflatoxin M1 over the concentration range of 1-14 ng/mL with a standard deviation of +/- 0.36 ng/mL Finally, the biosensor was applied to a series of real milk samples. (C) 2011 Elsevier B.V. All rights reserved.TUBiTAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106T 367]; EBiLTEM (Ege University of Research and Application Center of Science and Technology)Ege University [2007 BiL 013]This study was supported by TUBiTAK (The Scientific and Technological Research Council of Turkey, Project number: 106T 367) and EBiLTEM (Ege University of Research and Application Center of Science and Technology, Project number: 2007 BiL 013)

Immobilization of anti-aflatoxin B1 antibody by UV polymerization of aniline and aflatoxin B1 detection via electrochemical impedance spectroscopy

In the study, we investigated the practicality of the UV polymerization of aniline for anti-aflatoxin B1 antibody immobilization, and utilization of the resulting biosensor in the impedimetric determination of aflatoxin B1. The anti-aflatoxin B 1 antibody was physically immobilized on gold electrodes by UV polymerization of aniline at a fixed wavelength. The biosensor was based on specific interaction anti-aflatoxin B1 - aflatoxin B1 recognition and investigation of this recognition event by electrochemical impedance spectroscopy. A calibration curve was obtained in a linear detection range 1-20 ng/mL aflatoxin B1. Finally, the biosensor was applied to analysis of a real food sample.TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106 T 367]; EBBILTEM (Ege University of Research and Application Center of Science and Technology)Ege University [2007 BBIL 013]Financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 106 T 367) and EBBILTEM (Ege University of Research and Application Center of Science and Technology, Project number: 2007 BBIL 013) is gratefully acknowledged