Expression of Pro Inflammatory Cytokines and Anti-Chlamydia Antibody in Bacterial Ocular Infection.

Current study was done to investigate the bacterial causes of eye infection for 130 patients with different ages in Babylon city , the study includes swab for eye and sample of blood. Bacteriological diagnostic  for eye swab which taken from different site include Conjunctivitis, Dacryocystitis, Lidinfection showed variation in types and number of isolates . Gram positive bacterial isolates include Staphylococcus aureus 67 (38.06%), Which represent the predominating bacteria, Staphylococcus epidermidis 18 (10.22%), While gram negative bacteria represent Escherichia coli 40 (22.7%), Neisseriae gonorrhoeae 5 (2.84%). Detection of Chlamydia trachomatis reveals only one positive case  out of 42 eye infected case for anti Chlamydia IgG –IgM ( 3.7 %), Immunological test of Cytokine IL-6 in patient show increased in their concentration compared with healthy at P< 0.05 reached 8.52 pg/ml especially at age group 41-50. IFN-? also show increased in their concentration in patient than controls although, there is no significant differences between patient and control groups. . Key words ; IFN gamma , IL-6 , eye infection , bacterial isolates ,Chlamydia trachomati

Genetic Study of TORCH Infections in Women with Bad Obstetric History: Multiplex Polymerase Chain Reaction for Detection of Common Pathogens and Agents of Congenital Infections

To revealed the incidence of TORCH infections among pregnancy wastage in women which had bad obstetric history (BOH). METHODS: The study included 132 women with bad obstetric history. Genetic evaluation for TORCH infections was carried out by specific primers designed for that purpose using PCR method. RESULT: Toxoplasma was 36.36%, rubella 20.45%, cytomegalovirus 29.55% and herpes simplex virus 13.64%. Maximum number of cases of abortion 52 (39.39%), preterm labor 29 (21.96%) was associated with toxoplasma infection, early neonatal deaths 19 (14.39%) were maximally associated with toxoplasma and CMV infections. while congenital malformations 14 (10.6%) were evident maximally with toxoplasma infection and intrauterine death 8 (6.06%). CONCLUSIONS: Women with BOH are significantly higher in infection compared with that in control. A previous history of pregnancy wastage, genetic infestation using specific primers for TORCH agent’s detection and the serological reaction for TORCH infections during current pregnancy must be considered while managing BOH cases so as to reduce the adverse fetal outcome