6 research outputs found
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Obstacles to detecting isoforms using full-length scRNA-seq data
Abstract: Background: Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in a single cell, even when multiple isoforms were detected in matched bulk RNA-seq samples. However, these studies generally did not consider the impact of dropouts or isoform quantification errors, potentially confounding the results of these analyses. Results: In this study, we take a simulation based approach in which we explicitly account for dropouts and isoform quantification errors. We use our simulations to ask to what extent it is possible to study alternative splicing using scRNA-seq. Additionally, we ask what limitations must be overcome to make splicing analysis feasible. We find that the high rate of dropouts associated with scRNA-seq is a major obstacle to studying alternative splicing. In mice and other well-established model organisms, the relatively low rate of isoform quantification errors poses a lesser obstacle to splicing analysis. We find that different models of isoform choice meaningfully change our simulation results. Conclusions: To accurately study alternative splicing with single-cell RNA-seq, a better understanding of isoform choice and the errors associated with scRNA-seq is required. An increase in the capture efficiency of scRNA-seq would also be beneficial. Until some or all of the above are achieved, we do not recommend attempting to resolve isoforms in individual cells using scRNA-seq
Recommended from our members
Obstacles to detecting isoforms using full-length scRNA-seq data
Abstract: Background: Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in a single cell, even when multiple isoforms were detected in matched bulk RNA-seq samples. However, these studies generally did not consider the impact of dropouts or isoform quantification errors, potentially confounding the results of these analyses. Results: In this study, we take a simulation based approach in which we explicitly account for dropouts and isoform quantification errors. We use our simulations to ask to what extent it is possible to study alternative splicing using scRNA-seq. Additionally, we ask what limitations must be overcome to make splicing analysis feasible. We find that the high rate of dropouts associated with scRNA-seq is a major obstacle to studying alternative splicing. In mice and other well-established model organisms, the relatively low rate of isoform quantification errors poses a lesser obstacle to splicing analysis. We find that different models of isoform choice meaningfully change our simulation results. Conclusions: To accurately study alternative splicing with single-cell RNA-seq, a better understanding of isoform choice and the errors associated with scRNA-seq is required. An increase in the capture efficiency of scRNA-seq would also be beneficial. Until some or all of the above are achieved, we do not recommend attempting to resolve isoforms in individual cells using scRNA-seq
Recommended from our members
Obstacles to Studying Alternative Splicing Using scRNA-seq
Abstract
Background: Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in a single cell, even when multiple isoforms were detected in matched bulk RNA-seq samples. However, these studies generally did not consider the impact of dropouts or isoform quanti cation errors, potentially confounding the results of these analyses.
Results: In this study, we take a simulation based approach in which we explicitly account for dropouts and isoform quanti cation errors. We use our simulations to ask to what extent it is possible to study alternative splicing using scRNA-seq. Additionally, we ask what limitations must be overcome to make splicing analysis feasible. We nd that the high rate of dropouts associated with scRNA-seq is a major obstacle to studying alternative splicing. In mice and other well established model organisms, the relatively low rate of isoform quanti cation errors poses a lesser obstacle to splicing analysis. We find that different models of isoform
choice meaningfully change our simulation results.
Conclusions: To accurately study alternative splicing with single-cell RNA-seq, a better understanding of isoform choice and the errors associated with scRNA-seq is required. An increase in the capture e ciency of scRNA-seq would also be beneficial. Until some or all of the above are achieved, we do not recommend attempting to resolve isoforms in individual cells using scRNA-seq.JW was supported by a BBSRC DTP studentship BB/M011194/1. MH was funded by a core grant from the
Wellcome Trust. AFS was funded by the Medical Research Council, MR/R009791/1
Recommended from our members
Obstacles to detecting isoforms using full-length scRNA-seq data
Abstract: Background: Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in a single cell, even when multiple isoforms were detected in matched bulk RNA-seq samples. However, these studies generally did not consider the impact of dropouts or isoform quantification errors, potentially confounding the results of these analyses. Results: In this study, we take a simulation based approach in which we explicitly account for dropouts and isoform quantification errors. We use our simulations to ask to what extent it is possible to study alternative splicing using scRNA-seq. Additionally, we ask what limitations must be overcome to make splicing analysis feasible. We find that the high rate of dropouts associated with scRNA-seq is a major obstacle to studying alternative splicing. In mice and other well-established model organisms, the relatively low rate of isoform quantification errors poses a lesser obstacle to splicing analysis. We find that different models of isoform choice meaningfully change our simulation results. Conclusions: To accurately study alternative splicing with single-cell RNA-seq, a better understanding of isoform choice and the errors associated with scRNA-seq is required. An increase in the capture efficiency of scRNA-seq would also be beneficial. Until some or all of the above are achieved, we do not recommend attempting to resolve isoforms in individual cells using scRNA-seq
Anomalously High Proton Conduction of Interfacial Water
Water at the solid-liquid interface exhibits an anomalous ionic conductivity and dielectric constant compared to bulk water. Both phenomena still lack a detailed understanding. Here, we report radiofrequency measurements and analyses of the electrodynamic properties of interfacial water confined in nano-porous matrices formed by diamond grains of various sizes, ranging from 5 nm to 0.5 μm in diameter. Contrary to bulk water, the charge-carrying protons/holes in interfacial water are not mutually screened allowing for higher mobility in the external electric field. Thus, the protonic conductivity reaches a maximum value, which can be five orders of magnitude higher than that of bulk water. Our results aid in the understanding of physical and chemical properties of water confined in porous materials, and pave the way to the development of new type of highly-efficient proton-conductive materials for applications in electrochemical energy systems, membrane separations science and nano-fluidics
Multi-sensor large-scale dataset for multi-view 3D reconstruction
We present a new multi-sensor dataset for 3D surface reconstruction. It
includes registered RGB and depth data from sensors of different resolutions
and modalities: smartphones, Intel RealSense, Microsoft Kinect, industrial
cameras, and structured-light scanner. The data for each scene is obtained
under a large number of lighting conditions, and the scenes are selected to
emphasize a diverse set of material properties challenging for existing
algorithms. In the acquisition process, we aimed to maximize high-resolution
depth data quality for challenging cases, to provide reliable ground truth for
learning algorithms. Overall, we provide over 1.4 million images of 110
different scenes acquired at 14 lighting conditions from 100 viewing
directions. We expect our dataset will be useful for evaluation and training of
3D reconstruction algorithms of different types and for other related tasks.
Our dataset and accompanying software will be available online