Existing drug resistance among Staphylococcus spp. from raw milk samples in Khon Kaen province, Northeastern Thailand by direct quadriplex PCR

To describe the proportion of methicillin resistant Staphylococcus aureus, and mupirocin resistant among the isolates\ud from milk, three hundred and eighty-one samples were collected in Khon Kaen province, Thailand, during January to March,\ud 2014. Quadriplex PCR was a method of choice. The occurrence of S. aureus and other Staphylococcus spp. were 21.26 and\ud 34.12%, respectively. Among the 81 S. aureus isolates, 82.72 (67/81), 11.11 (9/81), and 6.17% (5/81) were S. aureus, S. aureus\ud carrying mecA, and S. aureus harboring mupA genes, respectively. These two mutant genes may possibly be transferred to\ud other bacteria in milk. Therefore, good hygienic practices and strict control may limit the spread

Is water at farms a source of Campylobacter spp. contamination in live chickens in Khon Kaen Province of Thailand?

This study is the first to quantify the Campylobacter contamination in chicken farm waters. Water samples from 10 broiler chicken farms in Khon Kaen, Thailand, were collected during winter, summer, and rainy season. Four types of water i.e. 3 drinking water, 1 main tank water, 1 evaporative pond water, and 1 environmental water sample were collected at each farm twice during 1-15, and 16-35 days of rearing. In total, 360 water samples were sampled. The overall occurrence rate of contamination was 0.3% (1/360). Only 1 environmental water sample (1.7%, 1/60) was found contaminated with Campylobacter species. Water collected during the later stage of rearing (16-35 days) was positive for Campylobacter spp. contamination. The extent of contamination was greater than 230 MPN/100 ml. However, drinking and main tank water were free of Campylobacter spp. contamination