CELL PROLIFERATION AND MIGRATION ASSAY ON POLYMER SURFACES

Cell proliferation and migration on phosphonated polymer surfaces may impair cell adherence and migration. The aim of the present study was to evaluate the ability of osteoblast-like cells to migrate at the surface of the phosphonated chitosan pellicle compared to normal chitosan pellicle. Results. Cells proliferated and migrated at different rates according to surface charge. Negative surfaces showed lower adherence and the migration difference could be quantified. Conclusions. Cell migration on polymer surfaces can be successfully evaluated by improved scratch test performed directly on polymer pellicles. Migration assay can be quantified by measuring the scratch size following separator removal. At the same time, viability assays are applicable following this improved scratch test while with no cells are damaged by mechanical means mechanically damage

COMPARATIVE-EVALUATION-OF-TWO-VIABILITY ASSAYS ON BIODEGRADABLE POLYMER FOAMS FOR POTENTIAL BONE REGENERATION

The purpose of the present study was to evaluate comparatively the cytotoxicity of two foams prepared by phosphorylated cellulose and phosphorylated PVA using two common assays, MTT classic assay and Cell-titer blue assay. Two foams with potential applications for bone substitute were evaluated. Both evaluated samples (phosphorylated cellulose and phosphorylated PVA) conditioned as foams showed a very good compatibility against osteoblast-like cells. The main advantage of the cell titer blue protocol for cell proliferation against MTT assay is represented by the possibility of direct addition of the assay reagents onto the wells with the material to be evaluated