3,223 research outputs found
A cleavage map of bacteriophage Phi-X174 genome
Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave phi-X174 replicative form DNA into three sets of specific DNA fragments. The order of these fragments in the phi-X replicative form molecule was determined by (1) analysis of partial digest products, (2) analysis of overlapping sets of fragments produced by two different restrictive enzymes. On the basis of these results, a detailed physical map of the phi-X174 genome has been constructed with respect to the cleavage sites of all three enzymes
Production of Specific Fragments of {varphi}X174 Replicative Form DNA by a Restriction Enzyme from Haemophilus parainfluenzae, Endonuclease HP
A restriction endonuclease from Haemophilus parainfluenzae degrades {varphi}X174 replicative form DNA into eight specific fragments, ranging from 1,700 to 150 base pairs and terminated specifically by deoxycytidylic acid
One Size Does Not Fit All: Meeting the Health Care Needs of Diverse Populations
Proposes a framework for meeting patients' cultural and linguistic needs: policies and procedures that support cultural competence, data collection, population-tailored services, and internal and external collaborations. Includes a self-assessment tool
Memory reconsolidation in aversive and appetitive settings
Memory reconsolidation has been observed across species and in a number of behavioral paradigms. The majority of memory reconsolidation studies have been carried out in Pavlovian fear conditioning and other aversive memory settings, with potential implications for the treatment of post-traumatic stress disorder. However, there is a growing literature on memory reconsolidation in appetitive reward-related memory paradigms, including translational models of drug addiction. While there appears to be substantial similarity in the basic phenomenon and underlying mechanisms of memory reconsolidation across unconditioned stimulus valence, there are also notable discrepancies. These arise both when comparing aversive to appetitive paradigms and also across different paradigms within the same valence of memory. We review the demonstration of memory reconsolidation across different aversive and appetitive memory paradigms, the commonalities and differences in underlying mechanisms and the conditions under which each memory undergoes reconsolidation. We focus particularly on whether principles derived from the aversive literature are applicable to appetitive settings, and also whether the expanding literature in appetitive paradigms is informative for fear memory reconsolidation
Evidence of environmental strains on charge injection in silole based organic light emitting diodes
Using d. functional theory (DFT) computations, the authors demonstrated a
substantial skeletal relaxation when the structure of
2,5-bis-[4-anthracene-9-yl-phenyl]-1,1-dimethyl-3,4-diphenyl-silole (BAS) is
optimized in the gas-phase comparing with the mol. structure detd. from
monocrystal x-ray diffraction. The origin of such a relaxation is explained by
a strong environmental strains induced by the presence of anthracene entities.
Also, the estn. of the frontier orbital levels showed that this structural
relaxation affects mainly the LUMO that is lowered of 190 meV in the gas phase.
To check if these theor. findings would be confirmed for thin films of BAS, the
authors turned to UV photoemission spectroscopy and/or inverse photoemission
spectroscopy and electrooptical measurements. The study of the c.d. or voltage
and luminance or voltage characteristics of an ITO/PEDOT/BAS/Au device clearly
demonstrated a very unusual temp.-dependent behavior. Using a thermally
assisted tunnel transfer model, this behavior likely originated from the
variation of the electronic affinity of the silole deriv. with the temp. The
thermal agitation relaxes the mol. strains in thin films as it is shown when
passing from the cryst. to the gas phase. The relaxation of the intramol. thus
induces an increase of the electronic affinity and, as a consequence, the more
efficient electron injection in org. light-emitting diodes
Lattice Monte Carlo calculations for unitary fermions in a finite box
We perform lattice Monte Carlo simulations for up to 66 unitary fermions in a
finite box using a highly improved lattice action for nonrelativistic spin 1/2
fermions. We obtain a value of for the Bertsch
parameter, defined as the energy of the unitary Fermi gas measured in units of
the free gas energy in the thermodynamic limit. In addition, for up to four
unitary fermions, we compute the spectrum of the lattice theory by exact
diagonalization of the transfer matrix projected onto irreducible
representations of the octahedral group for small to moderate size lattices,
providing an independent check of our few-body simulation results. We compare
our exact numerical and simulation results for the spectrum to benchmark
studies of other research groups, as well as perform an extended analysis of
our lattice action improvement scheme, including an analysis of the errors
associated with higher partial waves and finite temporal discretization.Comment: Significant revisions from previous version. Included data at a
larger volume and performed an infinite volume extrapolation of the Bertsch
parameter. Published versio
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