Immunochemical Studies Of Measles Virus Nucleocapsids.

PhDMicrobiologyUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/178310/2/7822848.pd

Human endometrial stromal stem cells differentiate into megakaryocytes with the ability to produce functional platelets.

Human endometrium is a high dynamic tissue that contains endometrial stromal stem cells (hESSCs). The hESSCs have been differentiated into a number of cell lineages. However, differentiation of hESSCs into megakaryocytes (MKs) has not yet been investigated. The aim of this study was to investigate the feasibility of MK generation from hESSCs and subsequent production of functional platelets (PLTs). In our study, hESSCs were cultured from endometrial stromal cells as confirmed by positive stromal cell specific markers (CD90 and CD29) and negative hematopoietic stem cell markers (CD45 and CD34) expression. Then, hESSCs were differentiated in a medium supplemented with thrombopoietin (TPO) for 18 days. The MK differentiation was analyzed by flow cytometry and confocal microscopy. The differentiation medium was collected for PLT production analysis by flow cytometry, transmission electron microscopy and functional measurements. Our results show: 1) MKs were successfully generated from hESSCs as identified by expression of specific markers (CD41a: 1 ± 0.09% and 39 ± 3.0%; CD42b: 1.2 ± 0.06% and 28 ± 2.0%, control vs. differentiation) accompanied with reduction of pluripotent transcription factors (Oct4 and Sox2) expression; 2) The level of PLTs in the differentiation medium was 16 ± 1 number/µl as determined by size (2-4 µm) and CD41a expression (CD41a: 1 ± 0.4% and 90±2.0%, control vs. differentiation); 3) Generated PLTs were functional as evidenced by the up-regulation of CD62p expression and fibrinogen binding following thrombin stimulation; 4) Released PLTs showed similar ultra-structure characteristics (alpha granules, vacuoles and dense tubular system) as PLTs from peripheral blood determined by electron microscopic analysis. Data demonstrate the feasibility of generating MKs from hESSCs, and that the generated MKs release functional PLTs. Therefore, hESSCs could be a potential new stem cell source for in vitro MK/PLT production

Gynecological Findings at Laparoscopy for Tubal Sterilization in the Pre-Digital Era

Study Objective: Recent advances in computer science, digital photography and robotics have made seasoned gynecologists to miss and young physicians to be deprived of the opportunity to know about how laparoscopy was performed 30 years ago. Digital cameras were not available, computers were rudimental, optics was unsophisticated and instrumentation was minimal. Nevertheless patient care and accurate diagnosis were skillfully performed. The purpose of our study was to revisit gynecological findings at laparoscopy performed for tubal sterilization in the pre-digital era. Methods: We review the archives of laparoscopic photographs performed when interesting findings were documented, in patients who underwent laparoscopic tubal sterilization between 1982 and 1988. Interesting findings were defined as significant anatomic variations or pathologic results clearly documented. The equipment used for laparoscopy included a 10 mm 0 degree Laprocator (JHPIEGO) telescope, 12 cm Verres gas needle, 11 mm trocar with canula sleeve, carbon dioxide gas and Falope or Yoon rings with applicator. For the photography halogen light source, fiber optic cable extensor and 35 mm color Kodak crom film were used. Results: Thirty four photographs with interesting findings were identified. The pourpourri of pathology identified included: endometriosis, endometrioma, Allen Masters Syndrome, salpingitis isthmica nodosa, tuberculosis, intra peritoneal adhesions, conjoint ovaries, Lippes loop intraperitoneal, Stein-Leventhal Syndrome, failed tubal sterilization and hypoplastic uterus. Conclusions: We revisited interesting gynecological findings at laparoscopy performed for tubal sterilization in the pre-digital era

Gynecological Findings at Laparoscopy for Tubal Sterilization in the Pre-Digital Era

Study Objective: Recent advances in computer science, digital photography and robotics have made seasoned gynecologists to miss and young physicians to be deprived of the opportunity to know about how laparoscopy was performed 30 years ago. Digital cameras were not available, computers were rudimental, optics was unsophisticated and instrumentation was minimal. Nevertheless patient care and accurate diagnosis were skillfully performed. The purpose of our study was to revisit gynecological findings at laparoscopy performed for tubal sterilization in the pre-digital era. Methods: We review the archives of laparoscopic photographs performed when interesting findings were documented, in patients who underwent laparoscopic tubal sterilization between 1982 and 1988. Interesting findings were defined as significant anatomic variations or pathologic results clearly documented. The equipment used for laparoscopy included a 10 mm 0 degree Laprocator (JHPIEGO) telescope, 12 cm Verres gas needle, 11 mm trocar with canula sleeve, carbon dioxide gas and Falope or Yoon rings with applicator. For the photography halogen light source, fiber optic cable extensor and 35 mm color Kodak crom film were used. Results: Thirty four photographs with interesting findings were identified. The pourpourri of pathology identified included: endometriosis, endometrioma, Allen Masters Syndrome, salpingitis isthmica nodosa, tuberculosis, intra peritoneal adhesions, conjoint ovaries, Lippes loop intraperitoneal, Stein-Leventhal Syndrome, failed tubal sterilization and hypoplastic uterus. Conclusions: We revisited interesting gynecological findings at laparoscopy performed for tubal sterilization in the pre-digital era

Gynecological Findings at Laparoscopy for Tubal Sterilization in the Pre-Digital Era

Study Objective: Recent advances in computer science, digital photography and robotics have made seasoned gynecologists to miss and young physicians to be deprived of the opportunity to know about how laparoscopy was performed 30 years ago. Digital cameras were not available, computers were rudimental, optics was unsophisticated and instrumentation was minimal. Nevertheless patient care and accurate diagnosis were skillfully performed. The purpose of our study was to revisit gynecological findings at laparoscopy performed for tubal sterilization in the pre-digital era. Methods: We review the archives of laparoscopic photographs performed when interesting findings were documented, in patients who underwent laparoscopic tubal sterilization between 1982 and 1988. Interesting findings were defined as significant anatomic variations or pathologic results clearly documented. The equipment used for laparoscopy included a 10 mm 0 degree Laprocator (JHPIEGO) telescope, 12 cm Verres gas needle, 11 mm trocar with canula sleeve, carbon dioxide gas and Falope or Yoon rings with applicator. For the photography halogen light source, fiber optic cable extensor and 35 mm color Kodak crom film were used. Results: Thirty four photographs with interesting findings were identified. The pourpourri of pathology identified included: endometriosis, endometrioma, Allen Masters Syndrome, salpingitis isthmica nodosa, tuberculosis, intra peritoneal adhesions, conjoint ovaries, Lippes loop intraperitoneal, Stein-Leventhal Syndrome, failed tubal sterilization and hypoplastic uterus. Conclusions: We revisited interesting gynecological findings at laparoscopy performed for tubal sterilization in the pre-digital era