Efficacy of foliage fungicides against eyespot of winter wheat in Northern Italy

Summary. The efficacy of foliage fungicide applications against eyespot of soft wheat cv. Serio was evaluated under natural Oculimacula infection in an experimental area in the Po Valley (Northern Italy). The fungicide treatments prochloraz, prochloraz + propiconazole, and trifloxystrobin + cyproconazole were applied in the years of 2006 through to 2009. Seeds were also treated with a formulated product based on guazatine. All foliage fungicides were applied at the stem extension growth stage (Zadoks growth stage 30\u201232), and at the manufacturer recommended rates. All tested treatments reduced the disease severity compared with untreated control. Prochloraz alone and particularly in combination with propiconazole gave the greatest efficacy in reducing eyespot. All treatments increased grain yield in 2006 and 2008. The effects of treatments on some yield parameters were also examined

Water level forecasting through fuzzy logic and artificial neural network approaches

In this study three data-driven water level forecasting models are presented and discussed. One is based on the artificial neural networks approach, while the other two are based on the Mamdani and the Takagi-Sugeno fuzzy logic approaches, respectively. <P style='line-height: 20px;'> All of them are parameterised with reference to flood events alone, where water levels are higher than a selected threshold. The analysis of the three models is performed by using the <I>same input and output variables</I>. However, in order to evaluate their capability to deal with different levels of information, two different input sets are considered. The former is characterized by significant spatial and time aggregated rainfall information, while the latter considers rainfall information more distributed in space and time. <P style='line-height: 20px;'> The analysis is made with great attention to the reliability and accuracy of each model, with reference to the Reno river at Casalecchio di Reno (Bologna, Italy). It is shown that the two models based on the fuzzy logic approaches perform better when the physical phenomena considered are synthesised by both a limited number of variables and IF-THEN logic statements, while the ANN approach increases its performance when more detailed information is used. As regards the reliability aspect, it is shown that the models based on the fuzzy logic approaches may fail unexpectedly to forecast the water levels, in the sense that in the testing phase, some input combinations are not recognised by the rule system and thus no forecasting is performed. This problem does not occur in the ANN approach

DNA quantification to assess Zymoseptoria tritici on a susceptible cultivar of durum wheat to establish the best timing for fungicide application in an italian environment

Zymoseptoria tritici, a globally distributed pathogen, is responsible of Septoria tritici blotch (STB), one of the most damaging wheat diseases. In Italy the incidence of STB has increased during the past few years. The presence of Z. tritici on flag leaves of susceptible durum wheat plants, cultivar San Carlo, after a single artificial inoculation with two inoculum concentrations at different vegetative stages has been evaluated in the plain of Bologna (North of Italy), in a two year field study (2012–2013). The pathogen presence was also assessed in natural infection conditions after a fungicide application in the second year (2013). The results obtained, by visual examination (Incidence, Disease Severity) and DNA quantification by Real time PCR, demonstrated that BBCH 39 (flag leaf stage) is the most susceptible vegetative stage, independently of inoculum concentration and climatic conditions. A good correlation between Disease Severity and DNA quantity was observed in either sampling methods, entire flag leaves and flag leaf discs. Thereafter the most suitable period to obtain the best crop protection with only one fungicide treatment is the flag leaf stage

Differential Physiological Responses Elicited by Ancient and Heritage Wheat Cultivars Compared to Modern Ones.

Although ancient, heritage, and modern wheat varieties appear rather similar from a nutritional point of view, having a similar gluten content and a comparable toxicity linked to their undigested gluten peptide, whenever the role of ancient end heritage wheat grains has been investigated in animal studies or in clinical trials, more anti-inflammatory effects have been associated with the older wheat varieties. This review provides a critical overview of existing data on the differential physiological responses that could be elicited in the human body by ancient and heritage grains compared to modern ones. The methodology used was that of analyzing the results of relevant studies conducted from 2010 through PubMed search, by using as keywords "ancient or heritage wheat", "immune wheat" (protein or peptides), and immune gluten (protein or peptides). Our conclusion is that, even if we do not know exactly which molecular mechanisms are involved, ancient and heritage wheat varieties have different anti-inflammatory and antioxidant proprieties with respect to modern cultivars. It is, therefore, reasonable to assume that the health proprieties attributed to older cultivars could be related to wheat components which have positive roles in the modulation of intestinal inflammation and/or permeability

Measurement of surface velocity in open channels using a lightweight remotely piloted aircraft system

ABSTRACTIn this paper, a low-cost remotely piloted aircraft system (RPAS) technique is proposed for measurement of the surface velocity in rivers or channels with low surface velocity and small discharge. To verify the reliability of the results obtained with the RPAS, we simultaneously measured the surface velocity with other methods based on total stations and close range photogrammetry. The RPAS was used both with ground control points (GCPs) for orientation of the photographic images and without GCPs. The data analysis showed that the RPAS provides valid results even without GCPs. Use of a RPAS without GCPs, relying solely on flight altitude to determine the water velocity, opens the way for its utilization in emergency conditions when it is impossible to access the river banks for the realization and survey of GCPs

Efficacy of adalimumab as second-line therapy in a pediatric cohort of crohn’s disease patients who failed infliximab therapy: The Italian society of pediatric gastroenterology, hepatology, and nutrition experience

Background: Adalimumab (Ada) treatment is an available option for pediatric Crohn’s disease (CD) and the published experience as rescue therapy is limited. Objectives: We investigated Ada efficacy in a retrospective, pediatric CD cohort who had failed previous infliximab treatment, with a minimum follow-up of 6 months. Methods: In this multicenter study, data on demographics, clinical activity, growth, laboratory values (CRP) and adverse events were collected from CD patients during follow-up. Clinical remission (CR) and response were defined with Pediatric CD Activity Index (PCDAI) score ≤10 and a decrease in PCDAI score of ≥12.5 from baseline, respectively. Results: A total of 44 patients were consecutively recruited (mean age 14.8 years): 34 of 44 (77%) had active disease (mean PCDAI score 24.5) at the time of Ada administration, with a mean disease duration of 3.4 (range 0.3–11.2) years. At 6, 12, and 18 months, out of the total of the enrolled population, CR rates were 55%, 78%, and 52%, respectively, with a significant decrease in PCDAI scores (P&lt;0.01) and mean CRP values (mean CRP 5.7 and 2.4 mL/dL, respectively; P&lt;0.01) at the end of follow-up. Steroid-free remission rates, considered as the total number of patients in CR who were not using steroids at the end of this study, were 93%, 95%, and 96% in 44 patients at 6, 12, and 18 months, respectively. No significant differences in growth parameters were detected. In univariate analysis of variables related to Ada efficacy, we found that only a disease duration &gt;2 years was negatively correlated with final PCDAI score (P&lt;0.01). Two serious adverse events were recorded: 1 meningitis and 1 medulloblastoma. Conclusion: Our data confirm Ada efficacy in pediatric patients as second-line biological therapy after infliximab failure. Longer-term prospective data are warranted to define general effectiveness and safety in pediatric CD patients

Surface Modification of Carbon Nanotube Networked Films with Au Nanoclusters for Enhanced N O 2 Gas Sensing Applications

Multiwalled carbon nanotube (MWCNT) films have been deposited by using plasma-enhanced chemical vapor deposition (PECVD) system onto alumina substrates, provided with 6 nm thick cobalt (Co) growth catalyst for remarkably improved gas sensing, at working temperature in the range of 100–. Functionalization of the MWCNTs with nanoclusters of gold (Au) sputtering has been performed to modify the surface of carbon nanotube networked films for enhanced and specific gas detection up to sub-ppm level. It is demonstrated that the gas sensitivity of the MWCNT-based sensors depends on Au-loading used as surface-catalyst. The gas response of MWCNT-based chemiresistor is attributed top-typeconductivity in the Au-modified semiconducting MWCNTs with a very good short-term repeatability and faster recovery. The sensor temperature of maximum sensitivity of the Au-functionalized MWCNTs is found to decrease with increasing Au-loading on their surface, and continuous gas monitoring at ppb level of is effectively performed with Au-modified MWCNT chemiresistors

The lysine methyltransferase SMYD3 interacts with hepatitis C virus NS5A and is a negative regulator of viral particle production

Hepatitis C virus (HCV) is a considerable global health and economic burden. The HCV nonstructural protein (NS) 5A is essential for the viral life cycle. The ability of NS5A to interact with different host and viral proteins allow it to manipulate cellular pathways and regulate viral processes, including RNA replication and virus particle assembly. As part of a proteomic screen, we identified several NS5A-binding proteins, including the lysine methyltransferase SET and MYND domain containing protein 3 (SMYD3). We confirmed the interaction in the context of viral replication by co-immunoprecipitation and co-localization studies. Mutational analyses revealed that the MYND-domain of SMYD3 and domain III of NS5A are required for the interaction. Overexpression of SMYD3 resulted in decreased intracellular and extracellular virus titers, whilst viral RNA replication remained unchanged, suggesting that SMYD3 negatively affects HCV particle production in a NS5A-dependent manner. (C) 2014 The Authors. Published by Elsevier Inc

Vaccines in children with inflammatory bowel disease: Brief review

Incidence of inflammatory bowel diseases (IBDs), including Crohn’s disease (CD) and ulcerative colitis (UC), is increasing worldwide. Children with IBDs have a dysfunctional immune system and they are frequently treated with immunomodulating drugs and biological therapy, which significantly impair immune system functions and lead to an increased risk of infections. Vaccines are essential to prevent at least part of these infections and this explains why strict compliance to the immunization guidelines specifically prepared for IBD patients is strongly recommended. However, several factors might lead to insufficient immunization. In this paper, present knowledge on the use of vaccines in children with IBDs is discussed. Literature review showed that despite a lack of detailed quantification of the risk of infections in children with IBDs, these children might have infections more frequently than age-matched healthy subjects, and at least in some cases, these infections might be even more severe. Fortunately, most of these infections could be prevented when recommended schedules of immunization are carefully followed. Vaccines given to children with IBDs generally have adequate immunogenicity and safety. Attention must be paid to live attenuated vaccines that can be administered only to children without or with mild immune system function impairment. Vaccination of their caregivers is also recommended. Unfortunately, compliance to these recommendations is generally low and multidisciplinary educational programs to improve vaccination coverage must be planned, in order to protect children with IBD from vaccine-preventable diseases

Botulinum toxin A modifies nociceptive withdrawal reflex in subacute stroke patients

Objectives: The aims of this study were to evaluate the pattern of the nociceptive withdrawal reflex (NWR) of the upper limb at rest and after injection of Botulinum toxin type A (BoNT-A) in poststroke subacute hemiparetic patients. Methods: Fourteen patients with poststroke subacute hemiparesis underwent clinical and instrumental evaluation and BoNT-A injection. Painful electrical stimulation was applied to induce the NWR. Baseline EMG activity and NWR recordings (EMG and kinematic response) were performed at T0, one month (T1), and three months (T2) after the BoNT-A injection, as were Modified Ashworth Scale (MAS) and Functional Independence Measure (FIM) scores. Results: Comparison of results at T0, T1, and T2 revealed significant changes in the MAS score for the elbow (p&nbsp;&lt;&nbsp;0.001) and wrist joints (p&nbsp;&lt;&nbsp;0.001) and in the FIM score at T0 and T2. BoNT-A injection had a significant effect on both NWR amplitude and baseline EMG activity in the posterior deltoid (PD) and flexor carpi radialis (FCR) muscles as well as in all averaged muscles. Analysis of elbow kinematics before and after treatment revealed that the reflex probability rates were significantly higher at T1 and T2 than at T0. Conclusion: Injection of BoNT-A in the subacute phase of stroke can modify both the baseline EMG activity and the NWR-related EMG responses in the upper limb muscles irrespective of the site of injection; furthermore, the reflex-mediated defensive mechanical responses, that is, shoulder extension and abduction and elbow flexion, increased after treatment. BoNT-A injection may be a useful treatment in poststroke spasticity with a potential indirect effect on spinal neurons