Hypotheses: implementation of Le Chatelier’s principle as a potential integrative method to prevent and or cure coronavirus

The COVID-19 is an infectious disease caused by a newly discovered coronavirus (SARS-CoV-2) and is mainly considered a respiratory tract illness, even though it may influence other body functions. The virus spreads primarily through droplets of saliva or discharge from the nose when an infected person coughs or sneezes. The number of infected people and deaths associated with COVID-19 is increasing on a daily basis and the current third wave of the pandemic appears to be worse than the one that swept several countries over the summer. The only aspect that can be reasonably managed in this pandemic is the environmental stress, which is important to boost the patient’s immune system and the patient’s ability to recover. Here we propose a potential integrative method to prevent and or attempting to cure coronavirus patients using safe and available resources that can be administered easily by most patients. This treatment is taking advantage of the equilibrium Law as explained by Le Chatelier principle and implemented through the use of antiseptics/disinfectants that can be administered by inhalation without harming the patient’s health. This proposed method is expected to reduce the COVID-19 virus load to a threshold that could boost the patient’s immune system and speed recovery

Detection of Virulence Gene Profiles of Multi-Drug Resistance (MDR) in Pseudomonas aeruginosa Bacteria in Human

The spread of opportunistic bacteria Pseudomonas aeruginosa in hospitals correlated with several diseases to humans. This bacterium showed high resistance to many antibiotics due to their indiscriminate use, as well as mutations in the pathogenic genes of Pseudomonas aeruginosa, which are difficult to treat. Pseudomonas aeruginosa is transmitted to humans in hospitals as being special nosocomial bacteria through ventilators, also through food and water contaminated with these bacteria, which causes muscle fatigue, vomiting and nausea. It also weakens the immune system and is more severe in people who suffer from weak immunity, old age and children, and can lead to death. Therefore, scanning virulence genes in Pseudomonas aeruginosa helps understand pathogenesis mechanisms. The main objective of this study was to detect three pathogenic genes in these bacteria: ExoS, Apr, and Pich. Bacteriological samples were collected and detected by the Multiplex PCR mechanism. Results showed that the sizes of genes were: 444bp, 1017bp, and 608bp. The study of the reaction of multiplex polymers to detect genes (ExoS, Apr, Pich) in 32 colonies was provided by Al-Ahli Hospital in Hebron, the percentage of each gene was 95%. In addition to 12 samples provided by the Al-Istishari Hospital in the city of Ramallah, and the percentage of the presence of each gene in these samples 100%. In the antibiotic examination of Pseudomonas aeruginosa of 44 samples, the bacteria had antibiotic resistance, 43% and 31% of the bacterial strains were resistant to Gentamicin and Aztreonam, 27% of which were resistant to Meropenem, Ceftazidime and Ciprofloxacin, 18% of which were resistant to tazobactam and 22.7% of which were resistant to Amikacin. The minor objective was to identify MDR Pseudomonas aeruginosa from clinical isolates. Isolates were evaluated for their antimicrobial susceptibility to seven antibiotics, Meropenem, Ceftazidime, Amikacin, Azteronam, Ciprofloxacin, Gentamicin and Piperacillin-tazobactam. The results also provided a clear picture of bacterial resistance MDR to these antibiotics by 45.45%

Isolation of bacteria that are able to digest keratin as an alternative to harmful chemicals used in tanning industries

Abstract:Macroccocus is a bacteria that was isolated from a soil sample containing decaying hair and It was found to contain keratinase enzyme due to its high ability to digest keratin protein. The isolated bacteria by this study can be used as an alternative to harmful chemicals tanning processes represents a safe and, clean technology that achieves economic feasibility in the process of skin soaking and hair removal. Background: Leather tanning is considered one of the ancient professions that Hebron has been known for hundreds of years which is the only source of raw materials for Palestinian factories thatdepends on this commodity. In the past, the industry relied on environmentally friendly natural materials in tanning, but today they have become a source of pollution of soil and groundwater, especially after the entry of chemicals in this craft, such as chromium and some acids that infect humans and destroy the Palestinian environment because of the toxic gases that result from the process of soaking and hair removal. Objectives: 1. To Isolate keratinolytic bacteria from soil sample 2. To test the bacterial effectiveness for hair degradation 3. To identify and characterize of bacteria by sequencing tool. Methods: Soil sample containing decaying hair was collected from the industrial area in Hebron city and processed in the lab, bacteria were isolated by using spreading and streaking plate technique, their ability for hair degradation was tested by incubating bacterial colony, hair and water for several days, after the bacterial ability for hair degradation appeared bacterial isolate was identified by sequencing tool and the type of bacteria was determined by bioinformatics tool. Results: Isolated a bacterial strain that is able to digest keratin and was tested on several samples and proved to be effective in degrading hair. It has been welcomed by the Modern Leather Tanning Company (MLTC-Hebron), which has cooperated with us in this project. The strain was genotyped and appeared to belong to macroccocus, this is the first report to find an industrial value for such bacteria which is also safe for human and the environment. Conclusions: Among microbial isolate screened for keratin digestion by using soil sample, a newly isolated Macroccocusequipercicuswas found with a greater ability for hair degradation, the use of such bacteria as an alternative to harmful chemicals tanning processes represents a safe and, clean technology that achieves economic feasibility in the process of skin soaking and hair removal. but in the same time, the full sequence of these strains of bacteria is unknown and not significantly studied. Therefore, additional researches have to be done for characterization of this type of bacteri

Production and purification of IgY antibodies from chicken egg yolk

The isolation of polyclonal antibodies from the serum of immunized mammals has significantly contributed to scientific research and diagnosis. The fact that recent technologies allow the production of antibodies in the yolk of eggs laid by hens, has led to the development of an alternative method for antibody generation that is less stressful to animals. As hens are kept under almost all their natural conditions, antibodies are isolated from the collected eggs; this technology is expected to become an interesting alternative to the conventionally serum-based techniques that eventually require to sacrifice the animal.Here we present a modified protocol for the isolation of IgY antibodies from immunized chickens and provide comparison between two chicken breeds in relative to IgY yield per egg. Our results show the possibility of generating large quantities of highly pure IgY from chicken eggs and also show large differences in the yield of IgY production between the two studied breeds. The results of this work indicate that IgY technology can be used for the production of primary antibodies for immunological work and disease diagnosis. Keywords: IgY, Chicken egg yolk, Gel filtration chromatography, Salmonella typhimuriu

Cannabis and Its Permissibility Status

Cannabis has been used and misused to treat many disorders. Δ9-Tetrahydrocannabinol (THC) and cannabidiol (CBD) are the most important components of cannabis and could be used for recreational and medical purposes. The permissibility (Halal) status of cannabis is controversial, and its rational use is ambiguous. Global awareness and interest in cannabis use are increasing and its permissibility status, especially for recreational and medical purposes, needs to be addressed. Rationalizing the scientific value and Halal status of cannabis is useful for the rational use and maintenance of the compatible system. It is rare in Muslim countries to discuss the permissibility status of cannabis from the perspective of its value and composition. Using the analogy concept, the CBD component extracted from a cannabis plant in a clean and pure form is permissible to use in industry, particularly in cosmetics and pharmaceuticals. If THC component is present in 99%, the mixture is considered permissible as long as THC is not intentionally added or intentionally left, but is mainly present due to the limited capabilities and efficiency of the purification methods. However, any amount of THC prepared with the intention to be used as an intoxicant is considered non-Halal