TOXICITY EVALUATION OF CRANKCASE OIL IN RATS

The aim of this study was to investigate the effect of crankcase oil on the cellular and func-tional integrity of rat skin. Thirty (30) rats were randomly grouped into six viz groups A–F. Group A (base-line control) received 2 ml of distilled water. 2.5 %, 5.0 %, 7.5 %, and 10.0 % v/v of the crankcase oil were prepared using unused oil as solvent and 2 ml of the concentra-tions were topically administered to groups C–F respectively for seven consecutive days. Group B served as positive control and received 2 ml of the unused oil. The rats were sacri-ficed 24 hours after the last administration, and blood and part of the skin were collected. Al-kaline phosphatase (ALP), acid phosphatase (ACP), superoxide dismutase (SOD) and malondialdehyde level in the blood and skin samples collected were evaluated. Elemental analysis of the crankcase oil was also carried out. The result revealed high lead, iron and chromium levels. Blood lead concentration of rats was significantly (P<0.05) high after seven days of administration. ALP level in skin and serum increased significantly (P<0.05) with the concentration of crankcase oil. There was a significant decrease (P<0.05) in skin ACP activity while it increased significantly (P<0.05) in the serum. Similar results were observed in the SOD levels of the serum and the skin. The level increased significantly (P<0.05) in groups D–F when compared with controls. The MDA concentration of both serum and skin were signif-icantly (P<0.05) elevated. This suggests toxic potential of used lubricating oil and its potential predisposition to cance

TOXICITY EVALUATION OF CRANKCASE OIL IN RATS

The aim of this study was to investigate the effect of crankcase oil on the cellular and func-tional integrity of rat skin. Thirty (30) rats were randomly grouped into six viz groups A–F. Group A (base-line control) received 2 ml of distilled water. 2.5 %, 5.0 %, 7.5 %, and 10.0 % v/v of the crankcase oil were prepared using unused oil as solvent and 2 ml of the concentra-tions were topically administered to groups C–F respectively for seven consecutive days. Group B served as positive control and received 2 ml of the unused oil. The rats were sacri-ficed 24 hours after the last administration, and blood and part of the skin were collected. Al-kaline phosphatase (ALP), acid phosphatase (ACP), superoxide dismutase (SOD) and malondialdehyde level in the blood and skin samples collected were evaluated. Elemental analysis of the crankcase oil was also carried out. The result revealed high lead, iron and chromium levels. Blood lead concentration of rats was significantly (P<0.05) high after seven days of administration. ALP level in skin and serum increased significantly (P<0.05) with the concentration of crankcase oil. There was a significant decrease (P<0.05) in skin ACP activity while it increased significantly (P<0.05) in the serum. Similar results were observed in the SOD levels of the serum and the skin. The level increased significantly (P<0.05) in groups D–F when compared with controls. The MDA concentration of both serum and skin were signif-icantly (P<0.05) elevated. This suggests toxic potential of used lubricating oil and its potential predisposition to cance

Kinetics of angiotensin -1 converting enzyme inhibition and antioxidative properties of Azadirachta indica seed protein hydrolysates

Neem (Azadirachta indica) seed protein hydrolysates were investigated forin vitroantioxidant and angiotensin 1-converting enzyme (ACE)-inhibitory activities. Neem seed proteins were hydrolysed using pepsin, trypsin andAlcalase. The degree of pepsin hydrolysis of neem seed protein was significantly higher (p<0.05) than those oftrypsin and Alcalase hydrolysis. Proteolytic hydrolysis of the isolate resulted in hydrolysates with improved Arg/Lys ratio, with pepsin hydrolysates still being able to maintain an acceptable level of essential amino acidscomparable to that of the isolate. At 2.5 mg/mL, pepsin neem seed protein hydrolysate (NSPH) demonstrated thestrongest antioxidant activity with 67.15 % and 50.07 % DPPH- and superoxide anion radical-scavenging ac-tivities, respectively, while trypsin NSPH had the highest ferric-reducing power. Using N-[3-(2-furyl)acryloyl]-L-phenylalanyl-glycyl-glycine (FAPGG) as substrate, NSPHs strongly inhibited ACE (69.20–80.39 %) in aconcentration-dependent manner. Pepsin NSPH had higher ACE-inhibitory activity than trypsin and AlcalaseNSPHs. Kinetic studies showed the mechanism of ACE inhibition to be mixed-type withKivalues of 0.62, 0.84, 1.5for pepsin, trypsin and alcalase NSPH, respectively. These results suggest that NSPH can be used as a potentialnutraceutical with antioxidant capacity and inhibitory activity against AC