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Additional file 4: Figure S3. Biomass production and lipid yields of A. fumigatus growing on different carbon sources. A. fumigatus/NEC: A. fumigatus grown on FGB with no extra carbon source; A. fumigatus/GLU: A. fumigatus grown on FGB with 20% glucose; A. fumigatus/TWS: A. fumigatus grown on FGB with 1% TWS. Significance levels: *) p<0.05; **) p<0.01

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Additional file 1: Figure S1. Artistic representation of two strategies designed for secretion of FFAs to growth media: ‘FFA Secretion’ and ‘Green Recovery’ from Synechocystis PCC 6803 cells. Text in red represents genetic modifications of SD100 strains

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Additional file 9: Figure S8. The samples of A. fumigatus-SD100, A. fumigatus-SD256 and A. fumigatus-SD257 pellets. 1) A. fumigatus-SD100 (left), A. fumigatus-SD256 after CO2 limitation (right); 2) A. fumigatus-SD100 (left), A. fumigatus-SD257 after CO2 limitation (right)

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Additional file 3: Figure S2. Pelletization of A. fumigatus grown in liquid media. (A) A. fumigatus pellets grown on FGB with no extra carbon source (left); with 1% TWS (middle) and with 20% glucose (right); (B) A. fumigatus pellets grown on FGB with 20% glucose (viewed from bottom); (C) A. fumigatus pellets grown on FGB with 20% glucose (left) and with 1% TWS (right). Scale=5mm

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Additional file 8: Figure S7. Increase in biomass and lipid/Intracellular FFA yield in A. fumigatus-Synechocystis pellets after flocculation. (A) Increase in lipids in A. fumigatus/GLU-SD pellets compare to the additive yields of lipid/intracellular FFA and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. (B) Increase in lipids in A. fumigatus/TWS-SD pellets compare to the additive yields of lipid/intracellular FFAs and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. Significance levels: *) p<0.05; **) p<0.01

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Additional file 7: Figure S6. Environmental scanning electron microscopy of A. fumigatus and A. fumigatus-Synechocystis PCC 6803 associations. (A,B) A. fumigatus pellets growing in liquid media; (C) A. fumigatus-SD100 and (D) A. fumigatus-SD216 pellets

Flocculation efficiency of microalgal cells by <i>A. fumigatus</i>.

<p>Flocculation of microalgal cells by <i>A. fumigatus</i>/GLU pellets; (B) <i>A. fumigatus</i>/TWS pellets. Flocculation efficiency of <i>A. fumigatus</i> with all microalgal strains showed significance levels, p<0.01.</p

Concentrations of nutrients in 25% swine wastewater before and after treatment with <i>Thraustochytrid</i> sp and <i>T. chuii</i> and their pellets with <i>A. fumigatus</i>.

<p>Concentrations of nutrients in 25% swine wastewater before and after treatment with <i>Thraustochytrid</i> sp and <i>T. chuii</i> and their pellets with <i>A. fumigatus</i>.</p

Microscopic analysis of <i>A. fumigatus</i>-miroalgal interactions.

<p>Images of miroalgal cells attached to <i>A. fumigatus</i> filaments. A, B) <i>D. tetrioletta</i>; C,D) <i>T. chuii</i>; E,F): <i>P. subcapitata</i>; G,H) <i>T. chuii</i>. A,B,C,D,F,G,H: UV light images. Red spots represent chloroplast's fluorescence; E) bright-field image CV: cell walls. Scale = 20 µm.</p