The effect of aqueous and ethanolic extracts of Artemisia herba alba on human laryngeal carcinoma and murine mammary adenocarcinoma cell lines

The present study was carried out to evaluate the cytological effects of aqueous (AE) and ethanolic (EE) extracts of Artemisia herba alba on human laryngeal carcinoma (Hep-2) cell line and murine mammary adenocarcinoma (AMN-3) cell line in vitro. The cytological study performed simultaneously with cell growth assay. The results of study revealed concentration-dependent cytological changes like patchy growth inhibition, loss of confluent feature and cellular degeneration after exposure to the lowest concentrations (156.25 and 312.5 μg/ml). The early findings of cytolysis were seen after exposure to 625 μg/ml. While the highest concentrations (1250, 2500 and 5000 μg/ml) caused severe growth inhibition with marked cytolytic features including loss of cellular outlines, large numbers of dead cells and high content of cellular debris. In conclusion, the results of this study revealed the high cytological effect of Artemisia herba alba extracts on Hep-2 and AMN-3 cell lines in vitro

Clinico-Histopathological and Immunohistochemical Study of Ruminant’s Cutaneous Papillomavirus in Iraq

The papilloma viruses are constituted of double-stranded DNA and are a more common lesion in ruminant’s skin in Iraq. The p53 tumor suppressor protein reveals an essential role in cell cycle control. This study intends to investigate the clinical, histopathological, and immunohistochemical features of cutaneous papilloma in ruminants in Iraq. Samples had been collected from a total of 10 animals (three cattle, three goats, and four sheep) with multiple papillomatosis lesions. The samples were processed for histopathological and immunohistochemical techniques. Clinically, the lesions appeared as multiple various sizes (0.5–11 cm), cauliflower exophytic masses on different parts of the animal’s body. The histopathological features of the epidermis granular layer revealed perinuclear vacuolation (koilocytosis) accompanied by various degrees of hypergranulosis, hyperkeratosis, acanthosis, orthokeratosis, and parakeratosis. Strong positive reaction for papillomavirus antigen was seen in both epidermal basal and granular layers in the immunohistochemical investigation (IHC). Moreover, all papilloma lesions revealed an intense positive p53 reaction in cytoplasmic and perinuclear of the basal and parabasal layers. In conclusion, this study described the papillomavirus lesions in bovine, ovine, and caprine animals, which were found in different parts areas of the affected animals. All lesions show similar histopathological features with minor variations. PV antigen and p53 protein expression showed positive results in immunohistochemistry that can be used as diagnostic markers for ruminant’s papilloma