Long -term feeding with high plant protein based diets in gilthead seabream (Sparus aurata, L.) leads to changes in the inflammatory and immune related gene expression at intestinal level

[EN] Background: In order to ensure sustainability of aquaculture production of carnivourous fish species such as the gilthead seabream (Sparus aurata, L.), the impact of the inclusion of alternative protein sources to fishmeal, including plants, has been assessed. With the aim of evaluating long-term effects of vegetable diets on growth and intestinal status of the on-growing gilthead seabream (initial weight = 129 g), three experimental diets were tested: a strict plant protein-based diet (VM), a fishmeal based diet (FM) and a plant protein-based diet with 15% of marine ingredients (squid and krill meal) alternative to fishmeal (VM+). Intestines were sampled after 154 days. Besides studying growth parameters and survival, the gene expression related to inflammatory response, immune system, epithelia integrity and digestive process was analysed in the foregut and hindgut sections, as well as different histological parameters in the foregut. Results: There were no differences in growth performance (p = 0.2703) and feed utilization (p = 0.1536), although a greater fish mortality was recorded in the VM group (p = 0.0141). In addition, this group reported a lower expression in genes related to pro-inflammatory response, as Interleukine-1 beta (il1 beta, p = 0.0415), Interleukine-6 (il6, p = 0.0347) and cyclooxigenase-2 (cox2, p = 0.0014), immune-related genes as immunoglobulin M (igm, p = 0.0002) or bacterial defence genes as alkaline phosphatase (alp, p = 0.0069). In contrast, the VM+ group yielded similar survival rate to FM (p = 0.0141) and the gene expression patterns indicated a greater induction of the inflammatory and immune markers (il1 beta, cox2 and igm). However, major histological changes in gut were not detected. Conclusions: Using plants as the unique source of protein on a long term basis, replacing fishmeal in aqua feeds for gilthead seabream, may have been the reason of a decrease in the level of different pro-inflammatory mediators (il1 beta, il6 and cox2) and immune-related molecules (igm and alp), which reflects a possible lack of local immune response at the intestinal mucosa, explaining the higher mortality observed. Krill and squid meal inclusion in vegetable diets, even at low concentrations, provided an improvement in nutrition and survival parameters compared to strictly plant protein based diets as VM, maybe explained by the maintenance of an effective immune response throughout the assay.The research has been partially funded by Vicerrectorat d'Investigacio, Innovacio i Transferencia of the Universitat Politecnica de Valencia, which belongs to the project Aquaculture feed without fishmeal (SP20120603). 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Geographical patterns in abundance and population structure of Nephrops norvegicus and Parapenaeus longirostris (Crustacea: Decapoda) along the European Mediterranean coasts

The main characteristics concerning distribution, size structure and total mortality of two of the most important decapod crustaceans of commercial interest in the Mediterranean Sea, Nephrops norvegicus and Parapenaeus longirostris, are studied along the European Mediterranean coasts. The study is based on data collected during a series of six trawl surveys performed in spring from 1994 to 1999 from the Gibraltar Straits to the Aegean Sea. The population size structure identified in the different geographical sectors is analysed taking into account two bathymetric sectors: continental shelf and upper slope. Differences in N. norvegicus population demographic structure among geographical sectors, as well as in total mortality, appear to be highly related to different exploitation levels. Size structure in P. longirostris also shows a great heterogeneity throughout the different geographical sectors. Considering that both species are heavily exploited all along the studied area, the observed differences can be interpreted as different responses to exploitation related to the widely differing life history characteristics of the mentioned species. In fact, N. norvegicus is a long-lived, benthic, burrowing species with low growth and mortality rates, and P. longirostris an epibenthic short-lived species characterised by higher rates of growth and mortalityPeer reviewe

Geographical patterns in abundance and population structure of Nephrops norvegicus and Parapenaeus longirostris (Crustacea: Decapoda) along the European Mediterranean coasts

The main characteristics concerning distribution, size structure and total mortality of two of the most important decapod crustaceans of commercial interest in the Mediterranean Sea, Nephrops norvegicus and Parapenaeus longirostris, are studied along the European Mediterranean coasts. The study is based on data collected during a series of six trawl surveys performed in spring from 1994 to 1999 from the Gibraltar Straits to the Aegean Sea. The population size structure identified in the different geographical sectors is analysed taking into account two bathymetric sectors: continental shelf and upper slope. Differences in N. norvegicus population demographic structure among geographical sectors, as well as in total mortality, appear to be highly related to different exploitation levels. Size structure in P. longirostris also shows a great heterogeneity throughout the different geographical sectors. Considering that both species are heavily exploited all along the studied area, the observed differences can be interpreted as different responses to exploitation related to the widely differing life history characteristics of the mentioned species. In fact, N. norvegicus is a long-lived, benthic, burrowing species with low growth and mortality rates, and P. longirostris an epibenthic short-lived species characterised by higher rates of growth and mortalityPeer reviewe

Apparent nutrient digestibility and gastrointestinal evacuation time in European seabass (Dicentrarchus labrax) fed diets containing different levels of legumes

Nutrient digestibility and gastrointestinal evacuation time for diets containing two levels of legumes were determined in European seabass (initial wt. 150 g). Seven isonitrogenous (44 g crude protein 100 g- 1) and isoenergetic (20 kJ g- 1) extruded diets were tested. Experimental diets contained 15 g or 30 g 100 g- 1 of each legume including faba bean meal, (FB15, FB30), chickpea meal (CP15, CP30), field pea meal (FP15, FP30) and a control, wheat meal containing, diet. Inclusion of faba beans in diets significantly enhanced apparent digestibility coefficients (ADCs) of dietary protein and energy at both inclusion levels. Starch digestibility was significantly lower for the control and FP30 diet and significantly higher for the FB15 diet. Fat digestibility coefficients were elevated significantly for FB15, FB30, CP15, FP15 and FP30 diets, compared to the CP30 and the control diet. The incorporation of faba bean and chickpea greatly affected gastrointestinal evacuation time compared to fish fed the control diet. Longer passage times were observed when these legumes were included in the diet. Glucose serum peak value was delayed for FB30 and a slower decreasing rate was observed for the legume diets. The addition of legumes had a positive effect on physical properties of the pellets by increasing the hardness while water activity remained within the safety limits. The findings of this study suggest that the legumes tested are potential candidates for carbohydrate replacement (wheat) and, to a lesser extent, for protein substitution in diets for European seabass

Teriparatide Treatment in Patients with Pregnancy- and Lactation-Associated Osteoporosis

Pregnancy- and lactation-associated osteoporosis (PLO) is a rare disease, presenting in most cases with severe back pain due to low energy vertebral fractures (VFs). Our purpose was to assess the effect of teriparatide (TPTD) vs. conventional management on areal bone mineral density (aBMD) and trabecular bone score (TBS) in patients with PLO. A multicenter retrospective cohort study concerning premenopausal women with PLO. Nineteen women were treated with TPTD (20 mu g/day) (group A) plus calcium and vitamin D and eight women with calcium and vitamin D only (group B) for up to 24 months. The primary end-point was between group differences in lumbar spine (LS) and total hip (TH) aBMD, and TBS at 12 and 24 months. Patients in group A had sustained a median of 4.0 VFs (3-9) vs. 2.5 VFs (1-10) in group B (p = 0.02). At 12 months, patients on TPTD vs. controls achieved a mean aBMD increase of 20.9 +/- 11.9% vs. 6.2 +/- 4.8% at the LS (p < 0.001), 10.0 +/- 11.6% vs. 5.8 +/- 2.8% at the TH (p = 0.43), and 6.7 +/- 6.9% vs. 0.9 +/- 3.7% in TBS (p = 0.09), respectively. At 24 months, seven patients on TPTD and six controls achieved a mean LS aBMD increase of 32.9 +/- 13.4% vs. 12.2 +/- 4.2% (p = 0.001). P1NP levels during the first month of TPTD treatment were positively correlated with the 1-year LS aBMD change (r = 0.68, p = 0.03). No new clinical fractures occurred while on-treatment. In patients with PLO, TPTD treatment resulted in significantly greater increases in LS aBMD compared with calcium and vitamin D supplementation at 12 and 24 months

Modelling spatio-temporal patterns of fish community size structure across the northern Mediterranean Sea: an analysis combining MEDITS survey data with environmental and anthropogenic drivers

The state of marine systems subject to natural or anthropogenic impacts can be generally summarized by suites of ecological indicators carefully selected to avoid redundancy. Length-based indicators capture the status of fish community structure, fulfilling the Marine Strategy Framework Directive (MSFD) requirement for Descriptor 3 (status of commercial fish species). Although the MSFD recommends the development of regional indicators, a comparison among alternative length-based indicators is so far missing for the Mediterranean Sea. Using principal component analysis and dynamic factor analysis, we identified the most effective subset of length-based indicators, whether or not based on maximum length. Indicator trends and time series of fishing effort and environmental variables are also compared in order to highlight the individual and combined capability of indicators to track system changes across geographical sub-areas. Two indicators, typical length and mean maximum length, constitute the smallest set of non-redundant indicators, capturing together 87.45% of variability. Only in combination can these indicators disentangle changes in the fish community composition from modifications of size structure. Our study supports the inclusion of typical length among the regional MSFD Descriptor 3 indicators for the Mediterranean Sea. Finally, we show dissimilarity between the western and eastern-central Mediterranean, suggesting that there are sub-regional differences in stressors and community responses.Generalmente, el estado de los sistemas marinos sujetos a impactos naturales o antropogénicos puede ser resumido mediante un conjunto de indicadores ecológicos, cuidadosamente seleccionados para evitar la redundancia. Los indicadores basados en la talla reflejan el estado de la estructura de la comunidad de peces, cumpliendo el requisito de la Directiva Marco de la Estrategia Marina (MSFD) para el Descriptor 3 (estado de las especies de peces comerciales). Si bien MSFD recomienda el desarrollo de indicadores regionales, en el Mar Mediterráneo no se ha hecho hasta ahora una comparación entre los distintos indicadores disponibles basados en la talla. Mediante el análisis de componentes principales y el análisis de factores dinámicos, identificamos el subconjunto más eficaz de indicadores basados en la talla, estén o no basados en la talla máxima. Las tendencias de los indicadores y las series temporales del esfuerzo de pesca y las variables ambientales también son comparadas para resaltar la capacidad individual y combinada de los indicadores para detectar los cambios del sistema a través de las subáreas geográficas. Dos indicadores, Longitud Típica (TyL) y Longitud Máxima Media (MML), constituyen el conjunto más pequeño de indicadores no redundantes, captando juntos el 87.45% de variabilidad. Solo si se combinan, estos indicadores pueden discernir entre los cambios en la composición de la comunidad de peces y las modificaciones de la estructura de tallas. Nuestro estudio respalda la inclusión de TyL entre los indicadores regionales del descriptor 3 de MSFD para el mar Mediterráneo. Finalmente, mostramos diferencias entre el Mediterráneo occidental y el Mediterráneo centraloriental que sugieren diferencias subregionales en cuanto a factores impactantes y las respuestas de la comunidad