83 research outputs found
κ°μλ¦° μμ§μ λΉμ μ μνμμμ μ°λ£λͺ¨λΈ κ°μ
νμλ
Όλ¬Έ(μμ¬)--μμΈλνκ΅ λνμ :κΈ°κ³ν곡곡νλΆ,2002.Maste
νκ΅μΈ 건μ νμμμ λ¨νμ₯ μμΈμ B κ΄μΉλ£μ μν μμμΉ¨μ°©μ μκ°μ λ³ν μμμ λν κ΄μ°°
Thesis(master`s)--μμΈλνκ΅ λνμ :μνκ³Ό νΌλΆκ³Όνμ 곡,2005.Maste
λμ μ§μμ κ±°μ£Όνλ μ μλμΈ΅ λ§μ± μ μ μ§νμμ μꡬλ μ‘°μ¬
νμλ
Όλ¬Έ(μμ¬)--μμΈλνκ΅ λνμ :μνκ³Ό μλ£κ΄λ¦¬νμ 곡,2000.Maste
θζ©«ζ·Έι‘θζ代ζ¨εΊζ°ζζ―θΌη‘η©Ά
νμλ
Όλ¬Έ (λ°μ¬)-- μμΈλνκ΅ λνμ : κ΅μ΄κ΅λ¬Ένκ³Ό, 2011.2. κΆλν.Docto
Prevalence rates of mental disorders and health-seeking behaviors of the inmates in psychiatric nursing facilities and homeless asylums
νμλ
Όλ¬Έ(λ°μ¬)--μμΈλνκ΅ λνμ :μνκ³Ό μλ£κ΄λ¦¬ν μ 곡,2004.Docto
Valproic acid stimulates growth and regeneration of hairs through inhibition of GSK-3Ξ²
Human hair follicles are self-renewing structures, which undergo three phases of hair cycle; anagen, catagen, and telogen. It is well known that Ξ²-catenin, a transducer of Wnt signaling, is critical in development, growth, and regeneration of hair follicle. In the absence of Wnt signal, Ξ²-catenin is ordinarily phosphorylated in the cytoplasm by glycogen synthase kinase (GSK)-3 and then degraded by proteasome. Therefore, inhibition of GSK-3 is able to increase Ξ²-catenin and would be able to induce growth of hairs. Valproic acid (VPA) is an anticonvulsant and mood-stabilizing drug used for decades and is known to inhibit the GSK-3Ξ² in cancer cell line and neuronal cells. However, the effect of VPA on hairs has not been studied yet. In this study, VPA enhanced the proliferation of human dermal papilla (DP) cells and outer root sheath (ORS) cells in vitro. In addition, hair follicles treated with VPA showed more rapid elongation of hairs and remained longer in anagen stage. In DP cells treated with VPA, Ξ²-catenin protein was found to be increased and accumulated in the nucleus, but the mRNA level of Ξ²-catenin was not increased. Instead, GSK-3Ξ² was phosphorylated at Ser9 by VPA, which means inactivation of GSK-3Ξ². These findings suggest that VPA increases Ξ²-catenin by preventing its degradation, not by promoting the synthesis. Anagen hair induction using mice model showed accelerated regeneration of new hair coat, higher anagen induction score and thicker back skin in VPA-treated mice. VPA also enhanced the de novo neogenesis after wounding, which has been known as Wnt-dependent process. In conclusion, the results from this study suggest that VPA stimulates growth and regeneration of hairs in both of in vitro and animal model. The promoting effect of VPA on hair is mediated through inhibition of GSK-3Ξ² which prevents the degradation of Ξ²-catenin.νΈμ§μ νΈμλΏλ¦¬μΈκ°μΈν¬, κ²λΏλ¦¬μΈκ°μΈν¬, νΈμ λμΈν¬ λ± λ€μν μΈν¬λ€λ‘ μ΄λ£¨μ΄μ Έ μλ νΌλΆλΆμκΈ°μ΄λ€. νμκΈ°μ ννΌμΈν¬μ μ€λ°°μ½κΈ°μ μΈν¬μ μνΈμμ©μΌλ‘ λ°μνμ¬, μ΄μκ°λ λμ μ±μ₯κΈ°-ν΄νκΈ°-ν΄μ§κΈ°μ λ
νΉν μ£ΌκΈ°λ₯Ό 보μ΄λλ°, μ΄λ¬ν νΈμ§μ λ°μ λ° μ±μ₯μλ Wnt μ νΈ μ λ¬μμΈ Ξ²-cateninμ΄ μ€μν μν μ νλ€. Wnt μ νΈκ° μμ λμλ, Ξ²-cateninμ μΈν¬μ§μ GSK-3Ξ²μ μν΄ μΈμ°νλ ν λΆν΄λμ§λ§, Wntκ° μΈν¬λ§μ μμ©μ²΄μ κ²°ν©νλ©΄ GSK-3Ξ²λ₯Ό μ΅μ νμ¬ Ξ²-cateninμ λΆν΄λ₯Ό λ§λλ€. λ°λΌμ, GSK-3Ξ² μ΅μ μ λ Wnt μ νΈμ λ¬ μ²΄κ³μ νμ±μ μ΄λ©° νΈμ μ±μ₯ λ° μ¬μμ μ΄μ§ν κ°λ₯μ±μ΄ μλ€. μ μ λ
λμ νκ²½λ ¨μ λ‘ μ¬μ©λμ΄ μλ λ°νλ‘μ΅μ°λ GSK-3Ξ² μ΅μ μ λΌλ κ²μ΄ μ΅κ·Ό λ³΄κ³ λμμΌλ, μμ§ νΈμ μ±μ₯ λ° μ¬μμ λν μν₯μ μ°κ΅¬λ λ°κ° μλ€. λ°λΌμ, μ΄ μ°κ΅¬μμλ λ°νλ‘μ΅μ°μ΄ νΈμ μ±μ₯κ³Ό μ¬μμ λ―ΈμΉλ μν₯μ in vitro μ€νκ³Ό λλ¬Όλͺ¨λΈμ μ΄μ©νμ¬ νκ°νκ³ , λ°νλ‘μ΅μ°κ³Ό νΈμ λμΈν¬μ Ξ²-catenin λ°νμ κ΄λ ¨μ±μ μ°κ΅¬ν¨μΌλ‘μ¨ κ·Έ κΈ°μ μ λ°νκ³ μ νμλ€. λ°νλ‘μ΅μ°μ μ²λ¦¬νκ³ μ¬λ νΈμ λμΈν¬λ₯Ό λ°°μν κ²°κ³Ό, 1.0mM μ΄νμ λ°νλ‘μ΅μ°μ μν΄ μΈν¬ μ¦μμ΄ μ΄μ§λμμΌλ©°, 0.1mMμμ κ°μ₯ ν° ν¨κ³Όλ₯Ό 보μλ€. νΈμ§ κΈ°κ΄ λ°°μμμ, 0.1mM λ°νλ‘μ΅μ°μ νΈμ§μ μ±μ₯μ μ΄μ§νκ³ μ±μ₯κΈ°κ° λ μ€λ«λμ μ μ§λλλ‘ νμμΌλ©°, λ°°μλ νΈμ§μμ KI67 λ©΄μνκ΄μΌμμ μνν κ²°κ³Ό νΈμ λμ μ μ΄νκ³ μλ νΈκΈ°μ§ μΈν¬μ μ¦μμ΄ λλ ·μ΄ μ΄μ§λμλ€. λν, νΈμ λμΈν¬μ λ°νλ‘μ΅μ°μ μ²λ¦¬νλ©΄ Ξ²-catenin λ¨λ°±μ§μ΄ μ¦κ°νκ³ ν΅ λ΄μ μΆμ λμμΌλ, Ξ²-cateninμ mRNA λ°ν μ¦κ°λ κ΄μ°°λμ§ μμλ€. λμ , μΈν¬μ§μμ GSK-3Ξ²μ μΈμ°νκ° λλ ·νκ² μ¦κ°νμλ€. λ°λΌμ, λ°νλ‘μ΅μ°μ΄ νΈμ λ μΈν¬μμ Ξ²-cateninμ μ μ μ λ°ν λ° ν©μ±μ μ¦κ°μν€μ§λ μμ§λ§ GSK-3Ξ² μ΅μ νμ¬ Ξ²-cateninμ μ¦κ°μν΄μ μ μ μμλ€. C57BL/6 μμ₯λ₯Ό μ΄μ©ν μ±μ₯κΈ° μ λ μ€νμμ, λ°νλ‘μ΅μ°μ λν¬ν μμ₯λ€μμ μ±μ₯κΈ° μ λ μ μκ° λ λμκ³ νΌλΆμ λκ»λ λκΊΌμ λ€. λ§μ§λ§μΌλ‘, μμ² νμ ν볡 κ³Όμ μμ μ¬μλλ νΈμ§μ κ°μλ λ°νλ‘μ΅μ°μ λν¬ν μμ₯λ€μμ λ λ§μλ€. κ²°λ‘ μ μΌλ‘, λ°νλ‘μ΅μ°μ΄ νΈμ μ±μ₯κ³Ό μ¬μμ μ΄μ§ν μ μμμ in vitro μ λλ¬Ό μ€νμ ν΅ν΄ νμΈνμλ€. κ·Έλ¦¬κ³ , μ΄λ¬ν λ°νλ‘μ΅μ°μ ν¨κ³Όλ νΈμ λμΈν¬μμ GSK-3Ξ²λ₯Ό μ΅μ ν¨μΌλ‘μ¨ Ξ²-cateninμ λΆν΄λ₯Ό λ§κΈ° λλ¬ΈμΈ κ²μΌλ‘ μκ°λλ€.Docto
μμ‘°μ λ΄λ‘ ꡬμ±λ°©μ μ°κ΅¬
νμλ
Όλ¬Έ(μμ¬)--μμΈλνκ΅ λνμ :κ΅μ΄κ΅λ¬Ένκ³Ό κ΅λ¬Ένμ 곡,2001.Maste
Fast heuristic algorithms for the constrained paths moving target search problems (single searcher, search relocation, multiple searchers)
νμλ
Όλ¬Έ(λ°μ¬)--μμΈλνκ΅ λνμ :μ°μ
곡νκ³Ό,2008.2.Docto
Nuclear factor-kBκ° μμμΈν¬μ μ±μ₯κ³Ό μ¬λ©Έμ μ‘°μ νλ κΈ°μ
Thesis(doctors) --μμΈλνκ΅ λνμ :μνκ³Ό(ν΄λΆνμ 곡),2008. 8.Docto
λ§ν‘μ²λ₯μ λͺ λ μ λΆλ―Όκ° λΉκ΅ μ°κ΅¬
2011λ
2μ κ³ μ λ¬Έν λ°μ¬νμ λ
Όλ¬Έ, μ§λκ΅μ κΆλν μ μ
- β¦