서울시 임기제공무원제도를 중심으로

학위논문 (석사) -- 서울대학교 대학원 : 행정대학원 행정학과, 2020. 8. 정광호.오늘날 공공조직은 세계화와 정보화, 민주화의 시대 속에서 행정의 전문성 강화가 요구되고 있다. 1997년 동아시아 외환위기 이후 신자유주의적 행정개혁을 도입하며 공무원제도에도 성과와 경쟁을 도입하거나 민간 전문가를 유치하는 등 전문성을 강화하고 행정 효율성을 높이기 위한 다양한 제도들이 도입되었다. 이 중 조직성과 향상과 전문성 강화를 위한 대표적인 인사제도가 임기제공무원제도이다. 본 연구는 임기제 공무원 제도의 주요 이슈인 제도적 측면(채용제도, 계약기간), 운영적 측면(업무자율성, 성과평가), 행태적 측면(조직몰입, 직무만족)이 조직성과와 어떤 관계가 있는지 서울시 임기제공무원들을 대상으로 분석하였다. 연구결과 조직몰입, 채용제도, 직무만족, 성과평가, 업무자율성 순으로 조직성과에 긍정적인 영향을 미치는 것으로 나타났다. 특히 조직몰입과 직무만족을 포함하는 행태적 측면의 영향력이 큰 것으로 볼 수 있다. 반면 계약기간은 조직성과에 부정적인 영향을 미치는 것으로 나타났다. 이는 향후 임기제공무원제도의 방향에 있어 행태적 측면을 강화하고 신분불안정성을 해소하는 것이 필요함을 시사한다.Today, public organizations are required to strengthen the expertise of administration in the age of globalization, informatization, and democratization. Since the 1997 Asian financial crisis, various systems were instituted—such as neoliberal administrative reform to introduce performance and competition to the civil service system, or attraction of private experts—to enhance professionalism and increase administrative efficiency. Among them, the representative personnel system for improving organizational performance and strengthening expertise is the termed civil servants system. Under such issues, this study conducts a survey on the institutional aspects (recruitment system, contract period), operational aspects (work autonomy, performance evaluation), and behavioral aspects (organizational commitment, job satisfaction) of the termed civil servants system to analyze the relation between the three aspects and organizational performance. A survey is conducted on public officials who have a term of office in Seoul Metropolitan Government and analyze them through multiple return analysis. Research has shown that organizational commitment, recruitment system, job satisfaction, performance evaluation, and work autonomy have a positive impact on organizational performance. In particular, it can be seen that the influence of behavioral aspects including organizational commitment and job satisfaction to be substantial. On the other hand, the contract period had a negative impact on organizational performance. This suggests that it is necessary to strengthen behavioral aspects and to resolve identity instability in the future direction of the termed civil servants system.제 1 장 서론 1 제 1 절 연구의 배경 1 제 2 절 연구의 범위 및 주요내용 3 1. 연구의 대상과 범위 3 2. 연구의 주요 내용 5 제 2 장 이론적 배경 6 제 1 절 임기제 공무원 제도 6 1. 임기제 공무원 제도의 개념과 현황 6 2. 임기제 공무원 제도의 특성 11 3. 선행연구의 검토 14 제 2 절 임기와 공공조직 성과 17 1. 공공조직성과의 측정 17 2. 임기와 성과 간 관계 18 제 3 절 임기제공무원제도와 조직성과 22 1. 선행연구의 검토 22 2. 임기제공무원제도의 주요이슈 24 3. 기존연구와의 차별성 30 제 3 장 연구 설계 33 제 1 절 연구모형 33 제 2 절 변수의 설정 34 1. 종속변수 34 2. 독립변수 35 3. 통제변수 35 4. 설문조사 36 제 4 장 연구 결과 41 제 1 절 기초통계량 분석 41 1. 표본의 인구통계학적 현황 41 2. 변수의 기초통계량 분석 43 제 2 절 신뢰도 분석 46 1. 종속변수의 신뢰도 분석 46 2. 독립변수의 신뢰도 분석 47 제 3 절 일원배치 분산분석 48 1. 채용제도와 조직성과 49 2. 계약기간과 조직성과 53 3. 업무자율성과 조직성과 54 4. 성과평가와 조직성과 58 5. 조직몰입과 조직성과 62 6. 직무만족과 조직성과 67 제 4 절 상관관계 분석 73 제 5 절 다중회귀분석 75 1. 회귀분석 모형 75 2. 회귀분석 결과 78 제 6 절 가설검증 및 해석 79 1. 가설의 검증 결과 79 2. 가설 검증의 해석 81 제 5 장 결론 89 제 1 절 연구결과 요약 89 제 2 절 연구의 의의 및 정책적 제언 91 제 3 절 연구의 한계 및 향후과제 95 참고문헌 98 Abstract 103Maste

Disruption of CTCF/cohesin-mediated high-order chromatin structures by DNA methylation downregulates PTGS2 expression

학위논문 (박사)-- 서울대학교 융합과학기술대학원 분자의학 및 바이오제약학과, 2017. 8. 김태유.The CCCTC-binding factor (CTCF)/cohesin complex regulates gene transcription via high-order chromatin organization of the genome. De novo methylation of CpG islands in the promoter regions is an epigenetic hallmark of gene silencing in cancer. Although the CTCF/cohesin complex preferentially targets hypomethylated DNA, it remains unclear if CTCF/cohesin-mediated high-order chromatin structure is affected by DNA methylation during tumorigenesis. We found that DNA methylation down-regulates expression of prostaglandin-endoperoxidesynthase 2 (PTGS2), which is an inducible, rate-limiting enzyme for prostaglandin synthesis, by disrupting CTCF/cohesin-mediated chromatin looping. We show that the CTCF/cohesin complex is enriched near a CpG island associated with PTGS2. The PTGS2 locus forms chromatin loops through methylation-sensitive binding of the CTCF/cohesin complex. However, DNA methylation abolishes the association of the CTCF/cohesin complex with the PTGS2 CpG island. Disruption of chromatin looping by DNA methylation abrogates the enrichment of transcriptional components, such as positive elongation factor b, at the transcriptional start site of the PTGS2 locus. These alterations result in the down-regulation of PTGS2. Our results provide evidence that CTCF/cohesin-mediated chromatin looping of the PTGS2 locus is dynamically influenced by the DNA methylation status.INTRODUCTION 1 MATERIALS AND METHODS 4 RESULTS 11 DISCUSSION 34 REFERENCES 37 ABSTRACT IN KOREAN 44Docto

정치적 위기와 언론의 상징전략 : 노사정위원회에 대한 언론의 개입과 문제 정의

학위논문(석사)--서울대학교 대학원 :언론정보학과,2001.Maste

Technology of persuasion

학위논문 (석사)-- 서울대학교 대학원 : 인류학과, 2011.8. 박순영.Maste

Effects of constraint-induced movement using self-efficacy on the upper extremity function of hemiplegic patients

학위논문(박사)--서울대학교 대학원 :간호학과 간호학전공,2002.Docto

위장관암의 15번 염색체에서 넓은 범위에 걸쳐 발생하는 후성유전학적 변이에 의한 유전자의 발현 저해에 관한 연구

학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 분자의학 및 바이오제약학과, 2014. 2. 김태유.Cancer is a disease of genetic and epigenetic deregulation contributing to aberrant gene expression. Until recently, cancer associated epigenetic deregulation has been manifested as a local event affecting discrete genes. However, concurrent epigenetic silencing of a large chromosomal region encompassing multiple neighboring genes, termed as long range epigenetic silencing (LRES) is being newly identified. This kind of global epigenetic gene regulation may provide important implication in development of epigenetic based cancer treatment strategies. Therefor, we tried to identify novel LRES region in human gastrointestinal cancer and elucidate its importance in tumor progression. First, we compared microarray gene expression data of HCT116 and DKO to identify LRES region in human gastrointestinal cancer. As DKO is a derivative of HCT116 with DNMT1 and DNMT3b knocked out, difference between their gene expression implicates differential DNA methylation. Interestingly, we identified large chromosomal region with multiple genes at chromosome 15q23 showing low gene expression in HCT116 compared to DKO. Differential gene expression was confirmed with qRT-PCR. Contribution of　CpG island hypermethylation of gene promoters were verified with bisulfite sequencing. Histone modification, which is known as another important layer of epigenetic regulation, was also identified to regulate epigenetic silencing. Second, we examined the gene expression of 11 gastric cancer cells to verify whether the LRES was general event. We identified six gastric cancer cells showing comparable gene expression pattern with HCT116. To verify if LRES in gastric cancer cells were also regulated by DNA methylation and histone modification, we compared gene expression, methylation status, and histone modification of the genes within the LRES region. We confirmed that SNU601, which showed low level of gene expression along the LRES region, was associated with DNA methylation at gene promoters with MSP assay. SNU601 was also associated with low level of active histone marks, while repressive histone marks were enriched. Third, to validate how DNA methylation and histone modification interact to maintain silenced state of LRES region, we treated demethylating agent, 5-Aza-2-deoxycytidine (5-aza-CdR) to SNU601. We identified that gene restoration after the treatment was more likely a result of histone modification rather than demethylation of gene promoters. From these results we could suggest that maintaining DNA methylation contributes to inheriting stable silent state of LRES region, while plasticity of histone modification can result in transient gene expression change. Finally, to determine whether LRES of this region is clinically relevant event, we compared gene expression of five primary gastric tumor tissues with their normal pairs. We identified that genes within LRES region was silenced in primary gastric tissues. We concluded that this low gene expression was mediated by tumor specific DNA methylation at their promoters. Taken altogether, our results suggest a novel LRES region of chromosome 15q23 in human gastrointestinal cancer, mediated by tumor specific DNA hypermethylation and aberrant histone modification.CONTENTS ABSTRACT CONTENTS LIST OF TABLES LIST OF FIGURES INTRODUCTION METHODS 1. Cell culture and drug treatment 2. Reverse transcription PCR and Quantitative Real-Time RT-PCR (qRT-PCR) 3. Methylation-Specific PCR (MSP) and Bisulfite sequencing 4. Chromatin Immunoprecipitation Assay RESULTS 1. Identification of long range silencing of chromosome 15q23.2 mediated by epigenetic modification in HCT116. 2. Long range epigenetic silencing of chromosome 15q23.2 in gastric cancer cells. 3. Demethylating agent, 5-aza-CdR, induced gene reactivation and epigenetic modification. 4. LRES in primary gastric tumors. DISCUSSION36 REFERENCES ABSTRACT IN KOREANMaste

The Effect of CEO Outsiderness on Firm-Level Attentional Change

학위논문 (석사)-- 서울대학교 대학원 : 경영대학 경영학과, 2018. 2. 조승아.Despite the plethora of empirical studies on the effects of CEO succession on the organization, we know very little about the mechanism through which the origin of the new CEO impacts the strategic course of actions at the firm-level. Drawing from the attention-based perspective of firms, we argue that the relationship between the origin of a new CEO and organizational attention is influenced by the predecessors performance and status. Our empirical results suggest that firm with new outsider CEOs indeed exhibited more attentional change at the firm-levelhowever, the level of attentional change actually decreased when the predecessor CEOs performance was high. Moreover, we find that the status of the predecessor moderates this effect of prior performance. Specifically, an outsider CEO would lead to the lowest level of firm-level attentional shift when both predecessors performance and status are high. The results of this study shed light on the interlinkages between CEO turnover and transformation of organizational attention, as well as the role of executive status in the process.1. Introduction 1 2. Theory and Hypotheses 3 2.1. Attentional Change at the Organization Level 3 2.2. CEO Origin and Attentional Change at the Organization Level 4 2.3. The Moderating Role of the Predecessor 6 2.4. Predecessor Performance 7 2.5. Predecessor Status 9 2.6. Three-Way Interaction of New CEO Origin, Predecessor Performance and Status 11 3. Methods 12 3.1. Sample and Data Sources 12 3.2. Measures 12 3.2.1. Dependent Variable 12 3.2.2. Independent Variable 13 3.2.3. Moderating Variables 14 3.2.4. Control Variables 12 3.3. Data Analysis 16 4. Results 17 5. Discussion 26 References 31 국문초록 35Maste

Natural indigo dyeing on protein fibers

학위논문(박사)--서울대학교 대학원 :의류학과,2001.Docto

Regional Cerebral Blood Flow Abnormality Associated with Apathy and Depression in Dementia of the Alzheimer`s type

학위논문(석사) --서울대학교 대학원 :의학과 (핵의학전공),2009.8.Maste

Natural Pregnancy in Fertility Clinic: The Rhetoric For Constituting Boundary Between Nature and Culture

본고는 연구자의 석사논문(강지연 2011) 4장의 일부를 수정•보완한 것이다.재생산에 대한 보조생식기술의 개입은 한국에서 자연임신이라는 신조어를 만들어내고 있다. 기술이 직접 사용되는 불임 클리닉 뿐만 아니라 언론 매체에도 등장하기 시작한 이 복합어는 일견 어색하게 느껴지는데, 그간 임신은 그 자체로 자연의 영역으로 간주되어 왔으므로 앞에 자연이라는 수식어를 붙일 필요가 없었기 때문일 것이다. 그렇다면 자연임신이란 과연 무엇을 의미하며, 새로운 수식어가 갖는 효과는 무엇인가? 의학과 기술이 인공적으로 개입되지 않은 임신을 자연임신이라 일컫는다고 생각하면 답은 간단하게 보이지만, 자연분만이 언어사용 주체와 맥락에 따라 다르게 정의되듯이(정연보 2003), 자연임신 역시 다층적이고 유동적인 의미를 가지고 있으며, 그 속에서 자연과 인공의 경계가 재조정된다. 현재 이 단어가 가장 일상적으로 통용되는 공간은 바로 불임 클리닉이다. 이 곳에서 의사, 간호사, 환자는 대화를 나눌 때 끊임없이 자연임신에 대해서 이야기하는데, 대화 속에서 자연임신은 때로는 치료의 일환으로, 때로는 산과력의 일부로, 때로는 의료적 문제로 등장한다. 본 연구는 불임 클리닉을 하나의 문화적 세팅으로 파악하고 그 안에서 자연임신이 정의되는 맥락과 언어 속에 내포된 의미들을 추적함으로써, 자연임신과 보조생식기술의 관계, 그리고 더 나아가 자연과 기술의 관계를 고찰해보고자 한다. This paper examines the concepts of nature and culture in a clinical setting, focusing specifically on the tactical rhetoric of nature. Pregnancy has been regarded as a normal event in womens lives and an event beyond the human intervention. However, the appearance of assisted reproductive technology has changed the meaning of nature in the context of pregnancy, while enabling people to consider different methods to become pregnant. For this study, I conducted participant observation in a fertility clinic in South Korea for four months in 2010. During my research, I interviewed the staff members formally and informally, while observing the medical practices and the interactions between staffs and patients