A new approach to immunoFET operation

A new method is presented for the detection of an immunological reaction taking place in a membrane, which covers the gate area of an ISFET. By stepwise changing the electrolyte concentration of the sample solution, a transient diffusion of ions through the membrane-protein layer occurs, resulting in a transient membrane potential, which is measured by the ISFET. The diffusion rate is determined by the immobile charge density in the amphoteric protein layer, which changes upon formation of antibody—antigen complexes. No membrane potential is induced at zero fixed charge density as occurs at a protein characteristic pH. Isoeletric points of embedded proteins can be determined by detecting the zero potential response.\ud \ud Up to now, the authors have studied the membrane adsorption of lysozyme, human serum albumin (HSA) and the immune reaction of HSA with the antibody anti-human serum albumin (αHSA). The influence of protein parameters on the amplitude of the transient can be described with an empirical equation. Assuming Langmuir behaviour, the protein concentration in the solution can well be correlated with the concentration in the membrane.\ud \ud This new detection method is unique concerning direct measurements of charge densities and isoelectric points of amphoteric macromolecules adsorbed in the membrane. The simple procedure of one incubation stage followed by one detection stage, without separate washing and labelling techniques, gives direct information about specific charge properties of the macromolecules to be studied

µTransparent insulating channels as components for miniaturized chemical separation Devices

Currently, miniaturized devices that apply electro osmotic pumping or electrophoretic separations are mostly constructed by etching small insulating channels for supply and separation on glass substrates. In principle, silicon is a superior construction material in terms of inertness and design flexibility. However, because of its semiconducting properties, the use in high voltage applications like the ones mentioned above is quite limited. In this paper, the use of μTransparent Insulating Channel (μTIC) technology is demonstrated as a standard procedure to manufacture miniaturized analytical separation devices. This technique, μchannels having extremely thin, transparent and insulating walls can be fabricated. An overview of the impact of this technology is given, showing the advantages of a fabrication technology that is as flexible as silicon technology for the fabrication of μTAS or “lab on a chip” devices. The following basic technology and control parameters will be highlighted. 1. Up to 100 μm wide rectangular channels 2. Bosses and leak-free connections to external μ fluidics. 3. Web-like structures for inlets/outlets>100 μm. 4. Implementation of conductivity electrodes 5. Good thermal dissipation properties of the thin walls 6. Control of the electro osmotic flow by a radial voltage

Agent-Based Simulations of Monetary Policy and Financial Markets

This thesis contributes to the fields of monetary policy and financial market research by applying one of the most popular of emerging methodologies in economics, agent-based modelling (ABM), to four distinct research questions. ABM is a class of computer models in which the interactions of autonomous agents are simulated over time. In this thesis, I use ABMs to study the following questions. (1) How do central bank interest rate changes affect inflation in the short term? (2) Is it plausible that stock prices have become decoupled from their fundamental value? (3) How does stock market volatility affect wealth inequality? (4) How can central bank balance sheet policy best be used to stabilise asset prices? ABM simulations yield the following main results. (1) Interest rate changes have a small effect on inflation because interest rate pass-through to costs, consumption, investment, and bank lending is rather weak. (2) When simple mean-reversion trading strategies start eclipsing fundamentalist strategies, stock prices can start to deviate from their fundamentals, this would be hard to detect using the standard stock price statistics. (3) Stock trading tends to lead to a highly unequal state as the wealth of more and more traders becomes so low that they have to stop trading. Increasing price volatility accelerates the movement towards this state. (4) The central bank can stabilise asset prices without disturbing the average price if it commits to buying stocks that are too far below fundamental value and selling stocks that are too far above it

A microfluidic device for array patterning by perpendicular electrokinetic focusing

This paper describes a microfluidic chip in which two perpendicular laminar-flow streams can be operated to sequentially address the surface of a flow-chamber with semi-parallel sample streams. The sample streams can be controlled in position and width by the method of electrokinetic focusing. For this purpose, each of the two streams is sandwiched by two parallel sheath flow streams containing just a buffer solution. The streams are being electroosmotically pumped, allowing a simple chip design and a setup with no moving parts. Positioning of the streams was adjusted in real-time by controlling the applied voltages according to an analytical model. The perpendicular focusing gives rise to overlapping regions, which, by combinatorial (bio) chemistry, might be used for fabrication of spot arrays of immobilized proteins and other biomolecules. Since the patterning procedure is done in a closed, liquid filled flow-structure, array spots will never be exposed to air and are prevented from drying. With this device configuration, it was possible to visualize an array of 49 spots on a surface area of 1 mm2. This article describes the principle, fabrication, experimental results, analytical modeling and numerical simulations of the microfluidic chip.\ud \ud \ud \u