109 research outputs found

    Volontaire de gestion... Et pourquoi pas? Outils pour les volontaires de gestion dans l’action associative et culturelle

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    Les volontaires de gestion sont ceux qui s’impliquent et prennent des responsabilités dans les instances décisionnelles des associations ; leur rôle est donc essentiel pour entretenir et surtout maintenir la dynamique associative. Les difficultés auxquelles ils sont confrontés sont pourtant nombreuses : confusion des rôles entre assemblée générale, conseil d’administration et coordination/direction, manque d’information et d’outils, difficulté de mobiliser des personnes impliquées, manque de compétences et/ou de formation pour mener à bien leur rôle de membre et/ou d’administrateur, etc. Cette brochure entend apporter des réponses concrètes aux questions que se posent les volontaires de gestion engagés dans les organes de décision et de gestion des associations. Elle constitue un premier outil important pour apporter certains repères et aider les volontaires de gestion à aborder leur rôle de membre ou d’administrateur dans l’action associative et culturelle

    Pérennité des associations dans un environnement changeant. Vers la construction d’un outil de diagnostic à 360° pour les associations

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    En Belgique, le secteur associatif est un pilier important de la vie en société. Le contexte général dans lequel les associations mènent leurs activités est en profonde évolution. Ces évolutions sont sources d’opportunités mais elles créent aussi de l’incertitude et changent les règles du jeu. Cela nous amène à poser la question de la pérennité de chaque association, et du modèle associatif plus largement, à travers deux prismes - la légitimité et l’accès aux ressources -, pour explorer la capacité des associations à se préparer au futur, à anticiper les évolutions et à construire des réponses adaptées, sans perdre leurs spécificités. A l’initiative de CBC Banque et Assurance, cette étude a aussi mené à concevoir un outil de diagnostic à 360°, à destination des associations, qui porte sur dix dimensions de la vie associative. Ce rapport de recherche présente l’approche méthodologique ainsi que les étapes du cheminement réflexif théorique ayant mené à la construction de l’outil de diagnostic.Pérennité des ASBL dans un environnement changean

    Expression study by real-time quantitative RT-PCR of the Salmonella typhimurium mntH gene

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    The aim of our study was to compare the mntH expression of different Salmonella Typhimurium strains and other Salmonella serovars with real-time RT-PCR. Following the mntH expression in function of the growth showed that the mntH expression of S. Typhimurium is growth dependent. A strong decrease of the mntH expression is noticed when the growth reaches 1.78 108 CFU/ml. After induction with EDTA or H2O2, variations between different S. Typhimurium strains were observed. For some S. Typhimurium strains a 10 to 20 times higher mntH expression was noticed after H2O2 induction. The EDTA induction was for most strains lower (5 to 10 times) but also variations between different strains were observed. The other Salmonella serovars were strongly induced after H2O2 but not after EDTA induction

    The prevalence of Salmonella, Campylobacter and VTEC in pig farms

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    Four different pig farms were sampled for the prevalence of Salmonella, Campylobacter and VTEC. From a total of 215 rectal samples of individual pigs, 15 rectal samples taken from animals at the same farm were positive for Salmonella. The Salmonella status of the pigs at this farm differed from one age group to another. S. Typhimurium was isolated from all the rectal samples and S. Typhimurium and S. Schwarzengrund were isolated from the environment. On two other farms Salmonella was only present in the environment with S. London and S. Typhimurium as serotypes. With cut-off value %OD\u3e 40 in the ELISA we found a good correlation with the Salmonella status of the farm. The presence of Campylobacter was tested in 150 rectal swabs, 51 of these, spread over the four farms, turned out positive. All the strains were identified as Campylobacter coli by a species-specific PCR. To determine if pigs are a reservoir of VTEC a total of 289 samples were screened for the presence of VTEC and 54 strains were isolated that each carried one virulence gene. Thirty-one strains carried the vt2e variant of the vt2 gene, four strains harboured the hly A gene and 19 the eaeA gene

    The non-profit sector in Belgium. A quantitative and qualitative analysis - 2008 Ed.

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    This publication is a direct follow-up to and an update of the previous publication on organizations in Belgium that appeared at the end of 2005. The aim is to provide the general public with a dynamic description of the non-profit sector (associations) in Belgium via a quantitative and a qualitative analysis on the basis of the most recent data, while comparing it to previous data. A quantitative analysis of the sector has been made under three headings. The first chapter “The satellite account of the NPI” takes up and comments on the principal figures from the statistics generated by the National Bank with regards to what is known as “non-profit institutions”(NPI),associations and foundations. Production, contribution to the added value of the country, principal financial sources, covered costs,... form the main topics that are focussed on. The evolution of the sector over the period 2000 – 2004 is also outlined. The second chapter, “Employment in non-profit organizations”, focuses on the work factor in the nonprofit organizations that, in terms of salaried employment, represent the bulk of employment in the NPI’s. The third chapter, “Foundations in Belgium” focuses on the sector of the foundations that serve a public non-profit goal (the foundations beneficial to the public and the private foundations from which one can suppose that they serve a non-profit objective that goes beyond the pure private character). Strictly speaking, foundations are not associations, and yet they do have a number of things in common. They do not have a profit motive. Via their financing, they contribute in a broad sense to the dynamism of the sector and of civilian society. Chapter 3 paints a picture of this relatively unknown type of NPI that together do employ a few thousand people and pay out considerable sums of money. Chapter four, “The barometer of the civil society organizations in Belgium in the year 2007” forms the qualitative part of this study. It presents the results of a study that was carried out of 473 civil society organizations in 2007. The aim of the barometer is to get to know the points of view and experiences of the civil society organizations in the field covering a range of subjects: the recent introduction of the new laws concerning non-profit organizations and voluntary work and the evolution in the area of employment, volunteer work, members, sources of income,… Where in 2005 the barometer study was limited to the questioning of umbrella civil society organizations and federations of organizations, the study dug deeper to the level of local organizations, in such a way that will allow this barometer research to be repeated on a regular basis at a later date

    Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

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    The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamically-sound, truly LOG-linear qPCR reactions very quickly. The qPCR problems this invention solves are universal to all qPCR reactions, and it performs all necessary qPCR set-up calculations in about 52 seconds (using a pentium 4 processor) for up to seven qPCR targets and seventy-two samples at a time – calculations that commonly take capable investigators days to finish. We have named this custom Excel-based file system "FocusField2-6GallupqPCRSet-upTool-001" (FF2-6-001 qPCR set-up tool), and are in the process of transforming it into professional qPCR set-up software to be made available in 2007. The current prototype is already fully functional
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